中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (37): 6895-6898.doi: 10.3969/j.issn.1673-8225.2010.37.013

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

血管内皮生长因子和碱性成纤维细胞生长因子在缺血预处理大鼠局灶性脑缺血再灌注区域的表达

郭翠平1，王小月1，马如静2，薛红莲1，李亚军1   

  1. 迁安市人民医院，1儿科，2急诊科，河北省迁安市 064400
  • 出版日期:2010-09-10 发布日期:2010-09-10
  • 作者简介:郭翠平★，女，汉族，1974年生，硕士，2007年辽宁医学院毕业，主治医师，主要从事脑血管病发病基础研究。 gcp1974@qq.com

Expression of vascular endothelial growth factor and basic fibroblast growth factor in rat focal cerebral ischemia reperfusion region following ischemic preconditioning

Guo Cui-ping1, Wang Xiao-yue1, Ma Ru-jing2, Xue Hong-lian1, Li Ya-jun1   

  1. 1 Department of Pediatrics, 2 Deparment of Emergency, Qian’an People’s Hospital, Qian’an  064400, Hebei Province, China
  • Online:2010-09-10 Published:2010-09-10
  • About author:Guo Cui-ping★, Master, Attending physician, Department of Pediatrics, Qian’an People’s Hospital, Qian’an 064400, Hebei Province, China gcp1974@qq.com

PDF

361

被引次数

9

摘要：

背景：脑缺血预处理可增加碱性成纤维细胞生长因子的表达，可能导致脑缺血耐受的产生。大鼠大脑中动脉缺血再灌注给予血管内皮生长因子能够起到神经保护作用。
目的：观察缺血预处理对缺血再灌注大鼠血管内皮生长因子和碱性成纤维细胞生长因子表达的影响。
方法：将SD大鼠随机分为缺血预处理组、模型组和假手术组。缺血预处理及模型组线栓法阻塞大脑中动脉制备脑缺血模型。预处理组在脑缺血-再灌注前3 d用插入尼龙线阻塞大脑中动脉，缺血2 h后再灌注22 h。模型组第一次手术将线栓前推5 mm，不阻断血流，其他同预处理组。假手术组仅插入尼龙线不阻塞大脑中动脉。用苏木精-伊红染色法观察3组间神经细胞变化。用抗生物素-生物素-过氧化物酶复合物法检测各组血管内皮生长因子和碱性成纤维细胞生长因子蛋白的表达。分别比较3组神经功能评分、光镜下脑缺血再灌注区神经细胞形态、血管内皮生长因子和碱性成纤维细胞生长因子的表达。
结果与结论：与模型组比较，预处理组神经功能评分明显低于模型组(P < 0.01)。光镜下观察结果显示，与模型组比较，预处理组缺血面积及缺血程度均减轻，血管内皮生长因子和碱性成纤维细胞生长因子表达均明显升高(P < 0.05)。结果提示缺血预处理可能通过增强血管内皮生长因子和碱性成纤维细胞生长因子而对缺血再灌注大鼠神经细胞起保护作用。

关键词: 缺血预处理, 再灌注损伤, 血管内皮生长因子, 碱性成纤维细胞生长因子, 脑缺血, 组织构建

Abstract:

BACKGROUND: Cerebral ischemic preconditioning (IPC) can increase basic fibroblast growth factor (bFGF) expression, which may induce brain ischemic tolerance. Injection of vascular endothelial growth factor (VEGF) into cerebral ischemia/reperfusion region can play neuroprotective effect.
OBJECTIVE: To observe the effect of focal ischemic preconditioning on VEGF and bFGF expression in rat with cerebral ischemia reperfusion.
METHODS: Sprague Dawley rats were randomly divided into 3 groups. Middle cerebral artery occlusion (MCAO) and ischemic preconditioning model rats were established by thread ligation. The IPC group received IPC at 3 days and 2 hours of MCAO and rats were killed after 22 hours reperfusion; model group did not receive IPC; the control group received sham surgery two times. Nerve cell changes in the 3 groups were observed by haematoxylin-eosin staining. The expression of VEGF and bFGF protein was determined by avidin biotin-peroxidase complex method. Neurologic score and cerebral cortex changes and immunohistochemistry of VEGF and bFGF were compared in each group.
RESULTS AND CONCLUSION: Compared with the model group, the neurological deficit scores of the IPC group were obviously smaller (P < 0.01), the ischemia area and degree were lessened, but the expression of VEGF and bFGF were evidently increased (P < 0.05). Focal cerebral ischemic preconditioning can induce ischemic neuroprotective effect and its mechanism may be related with the expression of VEGF and bFGF.

中图分类号: