中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (11): 1959-1962.doi: 10.3969/j.issn.1673-8225.2010.11.015

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

压力性尿失禁动物模型的建立及效果评价

王皓羽1，杜小文2，徐剑炜1，朱永锋3，吴慧玲3，胡俊彪3，金 范3，吕蕊萍3   

  1. 复旦大学附属中山医院，1整形外科，3泌尿外科，上海市200032；
    2金华市人民医院泌尿外科，浙江省金华市  321000
  • 出版日期:2010-03-12 发布日期:2010-03-12
  • 通讯作者: 杜小文，主任医师，金华市人民医院泌尿外科，浙江省金华市 321000 dxwllp@163.com

Establishment and evaluation of an animal model of stress urinary incontinence

Wang Hao-yu1, Du Xiao-wen2, Xu Jian-wei1, Zhu Yong-feng3, Wu Hui-ling3, Hu Jun-biao3, Jin Fan3, Lü Rui-ping3   

  1. 1Department of Plastic Surgery, Zhongshan Hospital of Fudan University, Shanghai   200032, China;
    2Department of Urinary Surgery, Jinhua People’s Hospital, Jinhua   321000, Zhejiang Province, China;
    3Department of Urinary Surgery, Zhongshan Hospital of Fudan University, Shanghai   200032, China
  • Online:2010-03-12 Published:2010-03-12
  • Contact: Xu Jian-wei, Chief physician, Department of Plastic Surgery, Zhongshan Hospital of Fudan University, Shanghai 200032, China xu.jianwei@zs-hospital.sh.cn

PDF

645

被引次数

7

摘要：

背景：目前治疗压力性尿失禁的方法有药物治疗、物理-行为治疗及手术治疗等多种，但仍在探索优化中。
目的：探索通过截断双侧阴部神经及支配髂骨尾骨肌、耻骨尾骨肌的盆底神经肌友，建立稳定的压力性尿失禁动物模型的方法。
方法：6周龄SD雌性大鼠18只，体质量(199.44±8.41) g。随机分为3组，正常组、模型组和假手术组各6只。对模型组大鼠作双侧阴部神经及盆底神经肌支截断，假手术组分离暴露上述神经但不作截断，正常组大鼠不作特殊处理。术后2周测定3组大鼠的漏尿点压力。压力测定后取正常大鼠及模型大鼠的膀胱颈尿道交界部的横截面作组织学分析。
结果与结论：假手术组1只大鼠于术后1周死亡。余大鼠皆存活并顺利测定漏尿点压力。与正常大鼠相比，模型组大鼠平均漏尿点压力下降约33%(P < 0.05)，而假手术组大鼠与正常组大鼠平均漏尿点压力差异无显著性意义(P > 0.05)。组织学检查显示，与正常大鼠比较，模型组大鼠尿道横纹肌排列疏松，而肌纤维也出现一定的萎缩。提示截断双侧阴部神经及盆底神经肌支可建立较为稳定的压力性尿失禁动物模型。

关键词: 压力性尿失禁, 动物模型, 阴部神经, 漏尿点, 压力, 泌尿系统组织工程

Abstract:

BACKGROUND: Although drug treatment, physics-behavior therapy, and postoperative therapy have been commonly used to treat stress urinary incontinence (SUI), there is still no satisfactory treatment at present.
OBJECTIVE: To build a stable animal model simulating stress urinary incontinence (SUI) by bilateral transaction of pudendal nerve and nerves innervating pelvic floor muscles, including iliococcygeous muscle and pubococcygeous muscle.
METHODS: A total of 18 6-week-old female SD rats weighing (199.44±8.41) g were randomly divided into 3 groups: normal, model, and sham-surgery groups, with 6 rats in each group. Rats in the model group underwent bilateral transaction of pudendal nerves and nerves innervating iliococcygeous/pubococcygeous muscles, while rats in the sham-surgery group had same procedures except nerve transaction. The normal group did not undergo any operation. Each rat was subjected to measure leak point pressure (LPP) at 2 weeks after the operation. After the measurement of LPP, cross sections of connection area of bladder and urethra were sent to histology.
RESULTS AND CONCLUSION: One rat in the sham-surgery group died at 1 week after the operation. The LPP of model group decreased significantly by approximately 33% compared with the normal group (P < 0.05); however, there was no significant difference in LPP between sham-surgery and normal groups (P > 0.05). The results of histology showed loosely arrangement and atrophy of urethral striated muscle fibers in rats of the model group. Bilateral transaction of pudendal nerves and nerves innervating to iliococcygeous/ pubococcygeous muscles resulted in SUI in rats stably.

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