中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (11): 1955-1958.doi: 10.3969/j.issn.1673-8225.2010.11.014

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

纤维环穿刺法与纤维环切开法建立兔椎间盘退变模型

罗  平，刘玉林，陈  仲，林荔军，许  勇，黄小军，周初松   

  1. 南方医科大学珠江医院骨科，广东省广州市510280
  • 出版日期:2010-03-12 发布日期:2010-03-12
  • 通讯作者: 周初松，主任医师，副教授，南方医科大学珠江医院骨科，广东省广州市510280 zcsmd@yahoo. com.cn
  • 作者简介:罗 平，男，1984年生，湖南省长沙县人，汉族，南方医科大学在读硕士，主要从事脊柱骨科学的研究。 Luoping_lc@yahoo.com.cn

Comparison of rabbit degenerative intervertebral disc models constructed by anulus puncture and annulus incision

Luo Ping, Liu Yu-li, Chen Zhong, Lin Li-jun, Xu Yong, Huang Xiao-jun, Zhou Chu-song   

  1. Department of Orthopaedics,  Zhujiang Hospital of Southern Medical University, Guangzhou  510280, Guangdong Province, China
  • Online:2010-03-12 Published:2010-03-12
  • Contact: Zhou Chu-song, Chief physician, Associate professor, Department of Orthopaedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China zcsmd@yahoo.com.cn
  • About author:Luo Ping, Studying for master’s degree, Department of Orthopaedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China Luoping_lc@yahoo.com.cn

PDF

492

被引次数

26

摘要：

背景：椎间盘退行性变模型的建立，是研究椎间盘退行性变病理过程和尝试基因治疗等的基础，建立的动物模型要求与人类椎间盘退行性变具有相似性和可比拟性，但目前尚缺乏公认的最佳实验动物模型。
目的：比较纤维环穿刺法和纤维环切开法建立兔椎间盘退变动物模型的差异。
方法：将32只新西兰大白兔以数字表法随机分为纤维环穿刺组和纤维环切开组。经腹膜外入路暴露L3/4、L4/5、L5/6椎间隙，纤维环穿刺组采取针刺纤维环，纤维环切开组采取尖刀切开纤维环，控制穿刺或切开的深度及方向。术后2，4，12，20周通过MRI和组织病理学检查观察腰椎间盘髓核变性及组织病理情况。
结果与结论：术后4周，兔椎间盘髓核面积缩小，纤维环面积增大，髓核内T2加权像信号降低、变暗，椎间隙高度也开始下降，纤维环穿刺组T2信号强度评分较纤维环切开组低(P < 0.05)；随着时间的进展，兔椎间盘T2信号强度评分逐步增高，椎间隙逐渐变窄，术后20周椎间盘T2信号强度评分达最高，两组比较差异无显著性意义。随着时间的进展，两组髓核内细胞含量逐渐减少，纤维软骨形成。提示纤维环穿刺法和纤维环切开法均可成功建立椎间盘退变模型，但纤维环切开法椎间盘的退变程度较纤维环穿刺法剧烈，建立的模型过程可能不是人体椎间盘自然退变的模拟过程，纤维环穿刺法比较真实地模拟了人类椎间盘损伤后的退变过程。

关键词: 纤维环穿刺法, 纤维环切开法, 椎间盘退变, 动物模型, 骨组织工程

Abstract:

BACKGROUND: Constructing animal model of intervertebral disc degeneration which more faithfully mimics the pathologic hallmarks of human intervertebral disc degeneration can be a beneficial assistance for further intervertebral disc degeneration therapy. However, there is not an accepted optimal model for intervertebral disc degeneration study.
OBJECTIVE: To compare the rabbit model of degenerative intervertebral disc constructed by anulus puncture and anulus incision.
METHODS: Totally 32 New Zealand white rabbits were randomly divided into the anulus puncture group and annulus incision group. Intervertebral disc of L3-6 was exposed by extro-peritoneal approach, and the discs were injured by puncturing the anulus or cutting the anulus. The deep and direction were controlled. Pathological change of intervertebral disc was checked with MRI and histopathological examination at weeks 2, 4, 12, and 20 after operation.
RESULTS AND CONCLUSION: At week 4 after operation, the area of nucleus gelatinosus was deflated with enlarged anulus fibrosus, T2-weighted image (T2WI) declined, blurred, and the height of intervertebral space was also decreased, the grade of T2 value in the anulus puncture group was lower than that of the annulus incision group (P < 0.05); with time prolonged, T2 scores increased, and the intervertebral space narrowed, which reached a peak at week 20 after operation. The differences had no significance. The histological sections demonstrated that the cell content in nucleus pulposus was increased gradually. The rabbit model of intervertebral disc degeneration can be successfully constructed by the methods of anulus puncture and annulus incision. The degeneration of incision model is more severe than that of puncture model. Anulus puncture method can faithfully mimic intervertebral disc degeneration after damage in human being.

中图分类号: