中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (11): 1935-1938.doi: 10.3969/j.issn.1673-8225.2010.11.009

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

三种不同材料注射构建高眼压模型兔的比较

王凤云1，陆晓和1，白 浪2，张 静1，张彩霞1   

  1. 1 南方医科大学附属珠江医院眼科，广东省广州市   510282；
    2 南方医科大学附属南方医院眼科， 广东省广州市 510515
  • 出版日期:2010-03-12 发布日期:2010-03-12
  • 通讯作者: 陆晓和，博士，教授，主任医师，博士生导师，南方医科大学附属珠江医院眼科，广东省广东市 510282
  • 作者简介:王凤云，女，1967年生，河南省洛阳市人，汉族，南方医科大学附属珠江医院眼科在读博士，副主任医师，主要从事眼科青光眼方面的研究。 wangfyfy@163.com

Comparison of rabbit intraocular hypertension models prepared by three different material injections

Wang Feng-yun1, Lu Xiao-he1, Bai Lang2, Zhang Jing1, Zhang Cai-xia1   

  1. 1Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou  510282, Guangdong Province, China;
    2Department of Ophthalmology, Nanfang Hospotil, Southern Medical University, Guangzhou  510515, Guangdong Province, China
  • Online:2010-03-12 Published:2010-03-12
  • Contact: Lu Xiao-he, Doctor, Professor, Chief physician, Doctoral supervisor, Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China
  • About author:Wang Feng-yun, Studying for doctorate, Associate chief physician, Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China wangfyfy@163.com

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454

被引次数

9

摘要：

背景：目前对青光眼的发病机制、过程、损害的研究很多，但缺乏理想的青光眼模型。
目的：利用不同材料制作3种兔高眼压动物模型，并验证各种兔高眼压动物模型在青光眼研究以及临床中实用价值。
方法：选普通级健康新西兰白兔30只，随机数字表法把30只兔子随机分为3组，每组10只。将每只兔子的一眼作为实验眼，另一只眼作为对照眼。3组实验眼前房内分别注射自体血、甲基纤维素、长效气体C3F8材料；对照眼用同样的方法注射生理盐水，建立青光眼动物模型。记录注射前、注射后当时、24，36，48，72，96，120，168 h眼压变化。
结果与结论：三种材料均可诱发实验兔成为高眼压模型，3组兔子眼压均有明显升高(P < 0.05)，其升高的幅度及维持的时间不同。3种材料诱发高眼压3持续时间分别为1，3，7 d，重复上述注射眼压可再次升高，维持高眼压时间更久。3组动物眼压波动在1.86~6.65 kPa之间。眼前节出现轻度炎症反应。证实利用自血、甲基纤维素、C3F83种材料建立的3种兔眼模型均是理想的高眼压模型，具有方法简单，易于操作和控制等特点。依据研究的需要，可以选择不同的实验动物模型，可用于急、慢性青光眼的相关研究。

关键词: 高眼压, 动物模型, 青光眼, 眼科组织工程, 兔

Abstract:

BACKGROUND: Currently, there are many studies concerning the pathogenesis, process, and damage of glaucoma, however, there is not an ideal glaucoma model.
OBJECTIVE: To prepare rabbit intraocular hypertension models using three different material injections, and to verify the practical value of intraocular hypertension models.
METHODS: Thirty New Zealand rabbits were divided randomly into 3 groups, with 10 animals in each group. One eye of each rabbit was served as the experimental eyes and the other eye as control eyes. Autoblood, methyl cellulose, C3F8 was injected into the anterior chamber of the experimental eyes, and the normal saline was injected into the control eyes. The intraocular pressure (IOP) was monitored prior to injection and at hours 0, 24, 36, 48, 72, 96, 120 and 168 after injection.
RESULTS AND CONCLUSION: Intraocular hypertension models could be induced by injecting 3 kinds of materials, and the IOP was obviously increased after injection (P < 0.05), and the ranges and periods of increasing were varied. The periods of increasing of 3 materials were 1, 3 and 7 days, respectively, which could maintain for longer time for a second injection. The IOP ranged 1.86-6.65 kPa, and mild anterior segment inflammation could be found. The experiment demonstrated that intraocular hypertension models using three different material injections are ideal models, which is characterized by simple, reliable and controllable. The suitable model can be selected for acute or chronic glaucoma research.

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