中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (10): 1865-1868.doi: 10.3969/j.issn.1673-8225.2010.10.033

• 干细胞综述 • 上一篇    下一篇

肝干细胞移植在肝病治疗中的应用

徐桂娟1，贾连群2， 吴云海1，颜迎春1，陈 阳1   

  1. 1沈阳市第六人民医院，辽宁省沈阳市  110006；
    2辽宁中医药大学，辽宁省沈阳市  110032
  • 出版日期:2010-03-05 发布日期:2010-03-05
  • 作者简介:徐桂娟，女，1965年生，辽宁省人，汉族，硕士，副主任医师，主要从事干细胞方面的研究。 Xuguijuan123@163.com

Application of hepatic stem cell transplantation to liver disease treatment

Xu Gui-juan1, Jia Lian-qun2, Wu Yun-hai1, Yan Ying-chun1, Chen Yang1   

  1. 1Shenyang Municipal Sixth People’s Hospital, Shenyang   110006, Liaoning Province, China;
    2Liaoning University of Traditional Chinese Medicine, Shenyang   110032, Liaoning Province, China
  • Online:2010-03-05 Published:2010-03-05
  • About author:Xu Gui-juan, Master, Associate chief physician, Shenyang Municipal Sixth People’s Hospital, Shenyang 110006, Liaoning Province, China Xuguijuan123@163.com

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摘要：

背景：目前，肝移植存在供体短缺、手术损伤、手术并发症发生率高以及费用高昂等问题，而肝干细胞移植为终末期肝病的治疗提供了崭新的思路。
目的：介绍肝干细胞的来源与分类、肝干细胞移植治疗终末期肝病的研究现状及面临的问题，并展望了其临床应用前景。
方法：应用计算机检索中国期刊全文数据库(CNKI：1999/2009)和Medline database数据库(1999/2009)相关文献，检索词分别为“肝干细胞，肝脏疾病，移植”和 “hepatic stem cells，liver  disease， transplantation”，语言分别设定为中文和英文，共检索到87篇文章，阅读文题和摘要进行筛选，选择具有原创性，论点论据可靠且分析全面的与肝干细胞移植临床应用密切相关的文章，排除重复研究及质量较差的文献，最后纳入30篇进行总结综述。
结果与结论：肝干细胞可分为肝源性和非肝源性。肝源性肝干细胞包括肝卵圆细胞、成熟肝细胞和间充质干细胞等，非肝源性肝干细胞主要来源于胚胎干细胞、骨髓造血干细胞和胰腺干细胞等。目前肝干细胞治疗肝病的研究尚处在初期阶段，从肝干细胞的发现到分离、纯化、全面鉴定、体外培养、定向分化及临床试验等，仍存在许多难题需要研究解决。不过作为一种新兴的种子细胞来源，肝干细胞不但可替换受损组织，而且可刺激受体组织再生以达到自身修复的目的，所以相比现在临床常用的原位肝移植和生物人工肝而言，肝干细胞在治疗各种原因引起的肝脏疾病中都具有十分广阔的前景。

关键词: 肝干细胞, 肝脏疾病, 治疗, 移植, 综述文献

Abstract:

BACKGROUND: At present, the problems such as serious shortage of donor liver organs for transplantation, surgical injury, high incidence of surgical complications, as well as the high costs limit the development of liver transplantation, while the hepatic stem cell (HSC) transplantation provides a new pathway for the treatment of end-stage liver disease.
OBJECTIVE: To introduce the source and classification of HSCs, research progress and problems of HSC transplantation for treatment of end-stage liver disease, and the clinical application prospects of HSC transplantation.
METHODS: Articles were collected from CNKI and Medline database with the keywords of “hepatic stem cells, liver disease, transplantation” in both Chinese and English from 1999 to 2009. Among 87 articles, 30 were included according to inclusion and exclusion criteria. Following reading titles and abstracts, original articles, and articles closely related to HSC transplantation with reliable argument and evidence and general analysis were included. Articles of repetitive studies and poor quality were excluded.
RESULTS AND CONCLUSION: The HSC can be divided into liver-derived stem cells and non-liver-derived stem cells. Liver-derived stem cells include hepatic oval cells, mature liver cells and small hepatocyte-like progenitor cell. Non-liver-derived stem cells were mainly derived from embryonic stem cells, bone marrow hematopoietic stem cells and pancreatic stem cells. Currently, the research for the treatment of liver disease by HSC is still in its early stages. There are many difficult issues to be studied and solved in the discovery, separation, purification, comprehensive identification, cultivation, directed differentiation as well as clinical trials. However, as a new source of seed cells, HSC can not only replace the damaged tissue but can stimulate the receptor in tissue regeneration. Hence, compared with the clinical liver transplantation and bio-artificial liver, there are very bright future for the treatment of liver diseases by transplating HSC.

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