中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (10): 1869-1873.doi: 10.3969/j.issn.1673-8225.2010.10.034

• 干细胞综述 • 上一篇    下一篇

骨髓间充质干细胞的分离方法和生物学特性

李鲁生1，张 涵1，王成俊1，程洪斌1，2，安沂华1，2   

  1. 1首都医科大学，北京市神经外科研究所，北京市  100050；
    2武警总医院神经干细胞移植科，北京市  100039
  • 出版日期:2010-03-05 发布日期:2010-03-05
  • 通讯作者: 安沂华，博士，首都医科大学，北京市神经外科研究所，北京市 100050；武警总医院神经干细胞移植科，北京市 100039 riveran@163.com
  • 作者简介:李鲁生，男，1984年生，浙江省永嘉县人，汉族，首都医科大学，北京市神经外科研究所在读硕士，主要从事中枢神经损伤修复与再生的研究。 lilusheng_020925 @163.com
  • 基金资助:

    国家863“干细胞与组织工程”重大项目(2006AA02A115)；
    北京市自然科学基金资助项目(7092017)。

Isolation methods and biological characteristics of bone marrow mesenchymal stem cells

Li Lu-sheng1, Zhang Han1, Wang Cheng-jun1, Cheng Hong-bin1,2, An Yi-hua1,2   

  1. 1Beijing Neurosurgical Institute, Capital Medical University, Beijing  100050, China;
    2Department of Neural Stem Cell Transplantation, General Hospital of Chinese People’s Armed Police Forces, Beijing  100039, China
  • Online:2010-03-05 Published:2010-03-05
  • Contact: An Yi-hua, Doctor, Researcher, Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China; Department of Neural Stem Cell Transplantation, General Hospital of Chinese People’s Armed Police Forces, Beijing 100039, China riveran@163.com
  • About author:Li Lu-sheng, Studying for master’s degree, Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China lilusheng_020925@163.com
  • Supported by:

    the Major Program of National 863 Project, No. 2006AA02A115*; 
    the Natural Science Foundation of Beijing, No. 7092017*

PDF

631

被引次数

69

摘要：

背景：骨髓间充质干细胞具有自身增殖能力强、分化范围广，能够修复损伤的功能组织及免疫调节等功能特点，具有广阔的治疗前景。骨髓间充质干细胞的分离方法众多，在不同的生长时期和不同的生长条件下具有较大的表达差异，但目前尚无统一的鉴定方法。
目的：综述骨髓间充质干细胞的分离方法及其生物学特性，并比较骨髓间充质干细胞在有或无血清，分裂增殖前后，分化和诱导前后的表达差异。
方法：应用计算机检索2003-01/2009-06中国期刊全文数据库(http://dlib.cnki.net/kns50/)相关文章，检索词为“骨髓间充质干细胞，分离，培养，分化，表型，特点”，并限定文章语言种类为中文。同时计算机检索2003-01/2009-06 Pubmed数据库(http://www.ncbi.nlm.nih.gov/PubMed)相关文章，检索词为“bone marrow mesenchymal stem cells，isolation，culture，induce，marker，characterization”，并限定文章语言种类为English。共检索到文献237篇。
结果与结论：用全骨髓培养法获得的骨髓间充质干细胞与密度梯度离心法获得的相比，CD44的阳性表达率和CD34的阴性表达率都要低一些。然而，全骨髓培养法的骨髓间充质干细胞比密度梯度离心法的骨髓间充质干细胞具有细胞活性更高，细胞增殖速度更快，细胞汇合时间更早和传代时间更短等优势，至于其他的分离方法存在耗资多、实验设备要求高等缺点。目前对骨髓间充质干细胞的分离鉴定主要依靠以下几个条件：贴壁性，细胞表面分子阳性和阴性选择标记，自身增殖能力强，具有多向分化能力。骨髓间充质干细胞在有或无血清，分裂增殖前后，分化和诱导前后具有较大的表达差异。

关键词: 骨髓间充质干细胞, 分离方法, 生物学特性, 综述文献

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) have the properties involving high proliferation capability, widely distribution, functional tissue repair after injury, as well as immune modulation, by which bring us extensive therapeutic possibilities. There are plenty of methods for isolation of BMSCs, yet, BMSCs exhibit discrepancies in varied growth stage and culture conditions. Up to now, there has been no agreement about the identification methods for cultured BMSCs.
OBJECTIVE: To review the isolation methods and biological characteristics of BMSCs, and to compare the differential expression of BMSCs between in serum and serum-free medium, prior to and after proliferation, as well as before and after induction.
METHODS: A computer-based online search was performed using key words of “bone marrow mesenchymal stem cells, isolation, culture, induce, marker, and characterization” to find documents published in the database of CNKI (http://dlib.cnki.net/kns50/) or Pubmed (http://www.ncbi.nlm.nih.gov/PubMed) from January 2003 to June 2009. The languages were limited Chinese and English. A total of 237 literatures were searched by the computer. 
RESULTS AND CONCLUSION: The positive rates of CD44 and CD34 of BMSCs isolated by the whole bone marrow culture were smaller than that of the density gradient centrifugation. However, BMSCs isolated by the whole bone marrow culture were superior to those isolated by the density gradient centrifugation in cell viability, proliferation rate, confluence time, as well as generation time. Other methods for BMSCs isolation had drawbacks of large cost and high requirement of experimental equipments. Following conditions were used to identify BMSCs: cell adherence, cell surface molecule labeling, strong self-proliferation ability, as well as potentials multi-directional differentiation. BMSCs exhibit differential expression between in serum and serum-free medium, prior to and after proliferation, as well as before and after induction.

中图分类号: