中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (3): 424-428.doi: 10.3969/j.issn.1673-8225.2010.03.011

• 药物控释材料 drug delivery materials • 上一篇    下一篇

聚醚砜膜片磺化前后对β2 -微球蛋白的吸附能力：膜吸附能力随磺化度增加而发生变化吗？

马星宇1，孙晓青2，程莉萍3，孙树东1，乐以伦1，黄  嘉1，毛华毅4   

  1. 1四川大学高分子科学与工程学院医用高分子材料及人工器官系，四川省成都市  610065; 2四川省仁寿县人民医院特检科，四川省仁寿县 620500;   3 四川大学材料学院，四川省成都市  610065; 4 中国核动力研究设计院成都同位素应用研究所，四川省成都市  610041
  • 出版日期:2010-01-15 发布日期:2010-01-15
  • 通讯作者: 孙树东，博士，副教授，四川大学高分子科学与工程学院，四川省成都市 610065
  • 作者简介:马星宇★，男，1984年生，陕西省榆林市人，汉族，四川大学高分子科学与工程学院在读硕士，主要从事生物材料与人工器官研究。 onlyonly_0@tom.com

Sulfonation of polyethersulfone sheets effects on adsorbability of beta 2-microglobulin: Whether the adsorbability changes with increased sulfonation degree?

Ma Xing-yu1, Sun Xiao-qing2, Cheng Li-ping3, Sun Shu-dong1, Yue Yi-lun1, Huang Jia1, Mao Hua-yi4   

  1. 1 Department of Biomaterials and Artificial Organs, College of Polymer Science and Engineering, Sichuan University, Chengdu  　610065, Sichuan Province, China; 2 Department of Special Laboratory, Renshou People’s Hospital, Renshou  620500, Sichuan Province, China; 3 College of Material Science and Engineering, Sichuan University, Chengdu  610065, Sichuan Province, China; 4 Nuclear Power Institute of China, Chengdu  610041, Sichuan Province, China　
  • Online:2010-01-15 Published:2010-01-15
  • Contact: Sun Shu-dong, Doctor, Associate professor, Department of Biomaterials and Artificial Organs, College of Polymer Science and Engineering, Sichuan University, Chengdu 　610065, Sichuan Province, China
  • About author:Ma Xing-yu★, Studying for master’s degree, Department of Biomaterials and Artificial Organs, College of Polymer Science and Engineering, Sichuan University, Chengdu 　610065, Sichuan Province, China onlyonly_0@tom.com

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摘要：

背景：尿毒症患者在长期透析过程中会出现与透析密切相关的淀粉样变性病症，其主要激发因子是β2 -微球蛋白，如何去除血液中β2 -微球蛋白一直是研究人员关注的重点。
目的：观察不同磺化度、国产与进口来源的两种聚醚砜血液透析膜材在体外静态接触人血清时吸附去除β2 -微球蛋白的能力。
方法：采用放免法测定膜材在125 I-β 2-微球蛋白溶液和人血清中，于37 ℃孵育不同时间后对125 I-β2-微球蛋白和β2 -微球蛋白的吸附情况。
结果与结论：不同来源的聚醚砜膜材对125 I-β 2-微球蛋白的饱和吸附百分率由大到小依次是高磺化度聚醚砜>低磺化度聚醚砜>未磺化聚醚砜；但在血清孵育体系中，不同材料对β2-微球蛋白的吸附均在30 min 达到最大吸附值，最后稳定的吸附量由大到小依次是高磺化度聚醚砜>低磺化度聚醚砜>未磺化聚醚砜，不同来源的聚醚砜对β2-微球蛋白的总体趋势完全一致。说明通过用膜吸附方式去除β2-微球蛋白是一种有效的方法。对聚醚砜进行磺化改性可显著提高材料对β2 -微球蛋白的吸附去除量，而且随着磺化度增加，膜吸附β2-微球蛋白的量增加。

关键词: 磺化聚醚砜, &beta, 2 -微球蛋白, 吸附, 透析相关淀粉样变性病, 放射免疫测试, 生物材料

Abstract:

BACKGROUND: Dialysis-related amyloid may occur during long-term dialysis for patients with uraemia, of which the main evocator is β2 -microglobulin (β2 M); therefore, how to eliminate β2M from blood is always the focus of research.
OBJECTIVE: To observe ability of removal of β2 -microglobulin (β2M) from serum using two kinds of polyethersulfone (PES) membrane materials with various degrees of sulfonation.
METHODS: These materials were incubated in radio-labeled β2 M (125 I-β2M) solution and human serum respectively at appointed time at 37 ℃, and then the amounts of 125 I-β2M and serumβ2M adsorbed by materials were measured by radio immunoassay.
RESULTS AND CONCLUSION: In the 125 I-β2M system, amounts of 125 I-β2M adsorbed by the materials decreased in the following sequence PES with high degree of sulfonation > PES with low degree of sulfonation > PES, whatever the source of PES was. In the serum system, amounts of β2M adsorbed reached maximums at 30 minutes and the final adsorptions decreased in sequence of PES with high degree of sulfonation > PES with low degree of sulfonation > PES. Sulfonated PES removed β2M more than PES did and the adsorption of β2M increases with the increase in the degree of sulfonation. Its ability to remove significant amount of β2M may result in less β2M available for incorporation into amyloid. The use of sulfonated PES membranes may lessen the likelihood of development of dialysis-related amyloidosis, which remains a major source of morbidity for patients treated with long-term hemodialysis.

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