关键词: 人β-防御素3, 水凝胶, 温敏型, 牙周炎, 炎症因子

Abstract: BACKGROUND: Previous studies have shown that human beta-defensin 3 has significant antifungal, antibacterial, and antiviral activities and plays an important bridging role in linking innate and acquired immune responses. 
OBJECTIVE: To observe the effect of human beta-defensin 3 hydrogel on treatment of periodontitis in rats.
METHODS: Using Poloxamer 188 and 407 as the matrix, a blank hydrogel was constructed by cold solution. Human beta-defensin 3 hydrogel was prepared by mixing human beta-defensin 3 with the hydrogel. Twenty-five SD rats were randomly divided into five groups with five rats in each group: No treatment was given in the healthy group. The periodontitis model was constructed by the orthodontic ligature wire method in the periodontitis group, blank hydrogel group, minocycline hydrochloride group, and human beta-defensin 3 hydrogel group. 8 weeks after modeling, blank hydrogel, minocycline hydrochloride, and human β-defensin 3 hydrogel were injected into the buccal and palatal periodontal bags, once a week, and relevant tests were carried out after continuous administration for 4 weeks.
RESULTS AND CONCLUSION: (1) Compared with the healthy group, periodontal plaque index, gingival bleeding index, and periodontal probing depth were increased in the periodontitis group (P < 0.01). Compared with the periodontitis group, the periodontal plaque index, gingival bleeding index, and periodontal probing depth of rats were decreased in the minocycline hydrochloride group and the human beta-defensin 3 hydrogel group (P < 0.05). (2) Hematoxylin-eosin staining proved that the hydrogel was not toxic to the rat organism. (3) Stereomicroscopy and Micro CT showed that compared with the healthy group, the root exposure and the distance between enamel cementum boundary and alveolar crest of the periodontitis group were increased (P < 0.05). Compared with the periodontitis group, the root exposure and the distance between enamel cementum boundary and alveolar crest of rats were reduced in the minocycline hydrochloride group and human beta-defensin 3 hydrogel group (P < 0.05). (4) Hematoxylin-eosin, Masson, and tartrate-resistant acid phosphatase staining showed that periodontal inflammation was obvious, fiber structure was disordered and osteoclasts were active in the periodontitis group and blank hydrogel group, while periodontal inflammation was decreased, fiber arrangement was more regular, and osteoclasts were reduced in the minocycline hydrochloride group and human beta-defensin 3 hydrogel group. (5) qRT-PCR showed that compared with the healthy group, the mRNA expressions of interleukin 1β, tumor necrosis factor α, interleukin 6, and inducible nitric oxide synthase were increased in the periodontitis group (P < 0.05). Compared with the periodontitis group, the mRNA expressions of interleukin 1β, tumor necrosis factor α, interleukin 6, and inducible nitric oxide synthase in gingival tissue of rats were decreased in the minocycline hydrochloride group and human beta-defensin 3 hydrogel group (P < 0.05). (6) The results showed that human beta-defensin 3 hydrogel was able to attenuate inflammation in rat periodontal tissues by decreasing the relative expression of inflammatory factors and inhibiting osteoblasts. 

Key words: human beta-defensin 3, hydrogel, temperature sensitive type, periodontitis, inflammatory factor