中国组织工程研究 ›› 2024, Vol. 28 ›› Issue (10): 1599-1605.doi: 10.12307/2024.314
• 生物材料综述 biomaterial review • 上一篇 下一篇
张本妥,杨 昕
收稿日期:
2023-03-16
接受日期:
2023-05-27
出版日期:
2024-04-08
发布日期:
2023-08-21
通讯作者:
杨昕,副主任医师,副教授,硕士生导师,北京大学第一医院,北京市 100034
作者简介:
张本妥,男,2001年生,江西省上饶市人,汉族,北京大学医学部本科在读,主要从事软骨损伤修复研究。
Zhang Bentuo, Yang Xin
Received:
2023-03-16
Accepted:
2023-05-27
Online:
2024-04-08
Published:
2023-08-21
Contact:
Yang Xin, Associate chief physician, Associate professor, Master’s supervisor, First Hospital, Peking University, Beijing 100034, China
About author:
Zhang Bentuo, First Hospital, Peking University, Beijing 100034, China
摘要:
文题释义:
软骨组织工程:是指将种子细胞植入模拟软骨细胞外基质结构的凝胶/支架中,并将支架材料移植在缺损软骨部位,在生长因子作用下使种子细胞增殖分化、合成并分泌细胞外基质,以达到软骨修复的目的。
背景:关节软骨损伤修复是临床上亟待解决的难题,利用人工生物组织工程材料促进软骨再生修复是目前的研究热点之一。
目的:对人工生物材料在关节软骨损伤修复中应用的研究进展进行综述。结果与结论:天然材料包括胶原、明胶、丝素蛋白、壳聚糖、海藻酸等,具有良好的生物相容性、降解性;合成材料包括聚乙二醇、聚已内酯、聚乳酸等,具有良好的机械性能。材料的改性与复合能够弥补材料本身的缺陷,表现出更优秀的软骨修复能力。基于分层结构合成的多层支架在研究中相对少见,且该支架更多针对骨软骨损伤修复而非单纯的软骨损伤修复。目前支架研究集中在合成研发阶段,相应的临床试验较少,未来需要注重临床应用转化。
https://orcid.org/0000-0003-3781-2742(张本妥)
中国组织工程研究杂志出版内容重点:生物材料;骨生物材料;口腔生物材料;纳米材料;缓释材料;材料相容性;组织工程
中图分类号:
张本妥, 杨 昕. 人工生物材料在关节软骨损伤修复中的应用[J]. 中国组织工程研究, 2024, 28(10): 1599-1605.
Zhang Bentuo, Yang Xin. Application of synthetic and biological materials in articular cartilage repair[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(10): 1599-1605.
[1] HUBER M, TRATTNIG S, LINTNER F. Anatomy, biochemistry, and physiology of articular cartilage. Invest Radiol. 2000;35(10):573-580. [2] NEWMAN AP. Articular cartilage repair. Am J Sports Med. 1998;26(2):309-324. [3] ULRICH-VINTHER M, MALONEY MD, SCHWARZ EM, et al. Articular cartilage biology. J Am Acad Orthop Surg. 2003;11(6):421-430. [4] SIMON TM, JACKSON DW. Articular Cartilage: Injury Pathways and Treatment Options. Sports Med Arthrosc Rev. 2018;26(1):31-39. [5] JONES KJ, CASH BM. Matrix-Induced Autologous Chondrocyte Implantation With Autologous Bone Grafting for Osteochondral Lesions of the Femoral Trochlea. Arthrosc Tech. 2019;8(3):e259-e266. [6] DEKKER TJ, AMAN ZS, DEPHILLIPO NN, et al. Chondral Lesions of the Knee: An Evidence-Based Approach. J Bone Joint Surg Am. 2021;103(7):629-645. [7] RICHTER DL, SCHENCK RC JR, WASCHER DC, et al. Knee Articular Cartilage Repair and Restoration Techniques: A Review of the Literature. Sports Health. 2016;8(2):153-160. [8] HUNZIKER EB, LIPPUNER K, KEEL MJ, et al. An educational review of cartilage repair: precepts & practice--myths & misconceptions--progress & prospects. Osteoarthritis Cartilage. 2015;23(3):334-350. [9] RICARD-BLUM S. The collagen family. Cold Spring Harb Perspect Biol. 2011; 3(1):a004978. [10] KILMER CE, BATTISTONI CM, COX A, et al. Collagen Type I and II Blend Hydrogel with Autologous Mesenchymal Stem Cells as a Scaffold for Articular Cartilage Defect Repair. ACS Biomater Sci Eng. 2020;6(6):3464-3476. [11] VÁZQUEZ-PORTALATI NN, KILMER CE, PANITCH A, et al. Characterization of Collagen Type I and II Blended Hydrogels for Articular Cartilage Tissue Engineering. Biomacromolecules. 2016;17(10):3145-3152. [12] QI Y, ZHANG W, LI G, et al. An oriented-collagen scaffold including Wnt5a promotes osteochondral regeneration and cartilage interface integration in a rabbit model. Faseb j. 2020;34(8):11115-11132. [13] MATSUSHITA R, NAKASA T, ISHIKAWA M, et al. Repair of an Osteochondral Defect With Minced Cartilage Embedded in Atelocollagen Gel: A Rabbit Model. Am J Sports Med. 2019;47(9):2216-2224. [14] CAO R, XU Y, XU Y, et al. Development of Tri-Layered Biomimetic Atelocollagen Scaffolds with Interfaces for Osteochondral Tissue Engineering. Adv Healthc Mater. 2022;11(11):e2101643. [15] VOLZ M, SCHAUMBURGER J, FRICK H, et al. A randomized controlled trial demonstrating sustained benefit of Autologous Matrix-Induced Chondrogenesis over microfracture at five years. Int Orthop. 2017;41(4): 797-804. [16] BRITTBERG M, RECKER D, ILGENFRITZ J, et al. Matrix-Applied Characterized Autologous Cultured Chondrocytes Versus Microfracture: Five-Year Follow-up of a Prospective Randomized Trial. Am J Sports Med. 2018;46(6): 1343-1351. [17] SCHNEIDER U, RACKWITZ L, ANDEREYA S, et al. A prospective multicenter study on the outcome of type I collagen hydrogel-based autologous chondrocyte implantation (CaReS) for the repair of articular cartilage defects in the knee. Am J Sports Med. 2011;39(12):2558-2565. [18] KON E, DELCOGLIANO M, FILARDO G, et al. Novel nano-composite multilayered biomaterial for osteochondral regeneration: a pilot clinical trial. Am J Sports Med. 2011;39(6):1180-1190. [19] WEI W, MA Y, YAO X, et al. Advanced hydrogels for the repair of cartilage defects and regeneration. Bioact Mater. 2021;6(4):998-1011. [20] LIN H, BECK AM, SHIMOMURA K, et al. Optimization of photocrosslinked gelatin/hyaluronic acid hybrid scaffold for the repair of cartilage defect. J Tissue Eng Regen Med. 2019;13(8):1418-1429. [21] SUO H, LI L, ZHANG C, et al. Glucosamine-grafted methacrylated gelatin hydrogels as potential biomaterials for cartilage repair. J Biomed Mater Res B Appl Biomater. 2020;108(3):990-999. [22] LIU F, WANG X, LI Y, et al. Dendrimer-modified gelatin methacrylate hydrogels carrying adipose-derived stromal/stem cells promote cartilage regeneration. Stem Cell Res Ther. 2022;13(1):26. [23] GU L, LI T, SONG X, et al. Preparation and characterization of methacrylated gelatin/bacterial cellulose composite hydrogels for cartilage tissue engineering. Regen Biomater. 2020;7(2):195-202. [24] ZHANG W, CHEN R, XU X, et al. Construction of Biocompatible Hydrogel Scaffolds With a Long-Term Drug Release for Facilitating Cartilage Repair. Front Pharmacol. 2022;13:922032. [25] YU H, FENG M, MAO G, et al. Implementation of Photosensitive, Injectable, Interpenetrating, and Kartogenin-Modified GELMA/PEDGA Biomimetic Scaffolds to Restore Cartilage Integrity in a Full-Thickness Osteochondral Defect Model. ACS Biomater Sci Eng. 2022;8(10):4474-4485. [26] LI Q, XU S, FENG Q, et al. 3D printed silk-gelatin hydrogel scaffold with different porous structure and cell seeding strategy for cartilage regeneration. Bioact Mater. 2021;6(10):3396-3410. [27] HAGHIGHI P, SHAMLOO A. Fabrication of a novel 3D scaffold for cartilage tissue repair: In-vitro and in-vivo study. Mater Sci Eng C Mater Biol Appl. 2021;128:112285. [28] HU H, DONG L, BU Z, et al. miR-23a-3p-abundant small extracellular vesicles released from Gelma/nanoclay hydrogel for cartilage regeneration. J Extracell Vesicles. 2020;9(1):1778883. [29] JIANG X, XIU J, SHEN F, et al. Repairing of Subchondral Defect and Articular Cartilage of Knee Joint of Rabbit by Gadolinium Containing Bio-Nanocomposites. J Biomed Nanotechnol. 2021;17(8):1584-1597. [30] YANG R, ZHANG X, LIU J, et al. Functional gelatin hydrogel scaffold with degraded-release of glutamine to enhance cellular energy metabolism for cartilage repair. Int J Biol Macromol. 2022;221:923-933. [31] LI Y, LIU Y, GUO Q. Silk fibroin hydrogel scaffolds incorporated with chitosan nanoparticles repair articular cartilage defects by regulating TGF-β1 and BMP-2. Arthritis Res Ther. 2021;23(1):50. [32] YUAN T, LI Z, ZHANG Y, et al. Injectable Ultrasonication-Induced Silk Fibroin Hydrogel for Cartilage Repair and Regeneration. Tissue Eng Part A. 2021;27(17-18):1213-1224. [33] GHOLIPOURMALEKABADI M, SAPRU S, SAMADIKUCHAKSARAEI A, et al. Silk fibroin for skin injury repair: Where do things stand? Adv Drug Deliv Rev. 2020;153:28-53. [34] SUN W, GREGORY DA, TOMEH MA, et al. Silk Fibroin as a Functional Biomaterial for Tissue Engineering. Int J Mol Sci. 2021;22(3):1499. [35] RODRIGUEZ MJ, BROWN J, GIORDANO J, et al. Silk based bioinks for soft tissue reconstruction using 3-dimensional (3D) printing with in vitro and in vivo assessments. Biomaterials. 2017;117:105-115. [36] ZHANG X, LIU Y, LUO C, et al. Crosslinker-free silk/decellularized extracellular matrix porous bioink for 3D bioprinting-based cartilage tissue engineering. Mater Sci Eng C Mater Biol Appl. 2021;118:111388. [37] ZHANG W, ZHANG Y, ZHANG A, et al. Enzymatically crosslinked silk-nanosilicate reinforced hydrogel with dual-lineage bioactivity for osteochondral tissue engineering. Mater Sci Eng C Mater Biol Appl. 2021; 127:112215. [38] NI T, LIU M, ZHANG Y, et al. 3D Bioprinting of Bone Marrow Mesenchymal Stem Cell-Laden Silk Fibroin Double Network Scaffolds for Cartilage Tissue Repair. Bioconjug Chem. 2020;31(8):1938-1947. [39] ZHOU Z, CUI J, WU S, et al. Silk fibroin-based biomaterials for cartilage/osteochondral repair. Theranostics. 2022;12(11):5103-5124. [40] SHARAFAT-VAZIRI A, KHORASANI S, DARZI M, et al. Safety and efficacy of engineered tissue composed of silk fibroin/collagen and autologous chondrocytes in two patients with cartilage defects: A pilot clinical trial study. Knee. 2020;27(5):1300-1309. [41] CHEN W, XU Y, LI H, et al. Tanshinone IIA Delivery Silk Fibroin Scaffolds Significantly Enhance Articular Cartilage Defect Repairing via Promoting Cartilage Regeneration. ACS Appl Mater Interfaces. 2020;12(19):21470-21480. [42] SHEN K, DUAN A, CHENG J, et al. Exosomes derived from hypoxia preconditioned mesenchymal stem cells laden in a silk hydrogel promote cartilage regeneration via the miR-205-5p/PTEN/AKT pathway. Acta Biomater. 2022;143:173-188. [43] LI Z, ZHANG X, YUAN T, et al. Addition of Platelet-Rich Plasma to Silk Fibroin Hydrogel Bioprinting for Cartilage Regeneration. Tissue Eng Part A. 2020;26(15-16): 886-895. [44] ZHANG M, ZHANG F, LI C, et al. Application of Chitosan and Its Derivative Polymers in Clinical Medicine and Agriculture. Polymers (Basel). 2022; 14(5):958. [45] SULTANKULOV B, BERILLO D, SULTANKULOVA K, et al. Progress in the Development of Chitosan-Based Biomaterials for Tissue Engineering and Regenerative Medicine. Biomolecules. 2019;9(9):470. [46] LIN IC, WANG TJ, WU CL, et al. Chitosan-cartilage extracellular matrix hybrid scaffold induces chondrogenic differentiation to adipose-derived stem cells. Regen Ther. 2020;14:238-244. [47] ZUBILLAGA V, ALONSO-VARONA A, FERNANDES SCM, et al. Adipose-Derived Mesenchymal Stem Cell Chondrospheroids Cultured in Hypoxia and a 3D Porous Chitosan/Chitin Nanocrystal Scaffold as a Platform for Cartilage Tissue Engineering. Int J Mol Sci. 2020;21(3):1004. [48] KUMAR BYS, ISLOOR AM, KUMAR GCM, et al. Nanohydroxyapatite Reinforced Chitosan Composite Hydrogel with Tunable Mechanical and Biological Properties for Cartilage Regeneration. Sci Rep. 2019;9(1):15957. [49] LI P, FU L, LIAO Z, et al. Chitosan hydrogel/3D-printed poly(ε-caprolactone) hybrid scaffold containing synovial mesenchymal stem cells for cartilage regeneration based on tetrahedral framework nucleic acid recruitment. Biomaterials. 2021;278:121131. [50] SHAMEKHI MA, MIRZADEH H, MAHDAVI H, et al. Graphene oxide containing chitosan scaffolds for cartilage tissue engineering. Int J Biol Macromol. 2019; 127:396-405. [51] YU R, ZHANG Y, BARBOIU M, et al. Biobased pH-responsive and self-healing hydrogels prepared from O-carboxymethyl chitosan and a 3-dimensional dynamer as cartilage engineering scaffold. Carbohydr Polym. 2020;244:116471. [52] SHEN Y, XU Y, YI B, et al. Engineering a Highly Biomimetic Chitosan-Based Cartilage Scaffold by Using Short Fibers and a Cartilage-Decellularized Matrix. Biomacromolecules. 2021;22(5):2284-2297. [53] LI S, LIU J, LIU S, et al. Chitosan oligosaccharides packaged into rat adipose mesenchymal stem cells-derived extracellular vesicles facilitating cartilage injury repair and alleviating osteoarthritis. J Nanobiotechnology. 2021;19(1):343. [54] DEHGHAN-BANIANI D, MEHRJOU B, WANG D, et al. A dual functional chondro-inductive chitosan thermogel with high shear modulus and sustained drug release for cartilage tissue engineering. Int J Biol Macromol. 2022;205:638-650. [55] SHOUEIR KR, EL-DESOUKY N, RASHAD MM, et al. Chitosan based-nanoparticles and nanocapsules: Overview, physicochemical features, applications of a nanofibrous scaffold, and bioprinting. Int J Biol Macromol. 2021;167:1176-1197. [56] MARTINS EA, MICHELACCI YM, BACCARIN RY, et al. Evaluation of chitosan-GP hydrogel biocompatibility in osteochondral defects: an experimental approach. BMC Vet Res. 2014;10:197. [57] STANISH WD, MCCORMACK R, FORRIOL F, et al. Novel scaffold-based BST-CarGel treatment results in superior cartilage repair compared with microfracture in a randomized controlled trial. J Bone Joint Surg Am. 2013; 95(18):1640-1650. [58] FRAPPIER J, STANISH W, BRITTBERG M, et al. Economic evaluation of BST-CarGel as an adjunct to microfracture vs microfracture alone in knee cartilage surgery. J Med Econ. 2014;17(4):266-278. [59] LIU W, MADRY H, CUCCHIARINI M. Application of Alginate Hydrogels for Next-Generation Articular Cartilage Regeneration. Int J Mol Sci. 2022; 23(3):1147. [60] LIU J, FANG Q, LIN H, et al. Alginate-poloxamer/silk fibroin hydrogels with covalently and physically cross-linked networks for cartilage tissue engineering. Carbohydr Polym. 2020;247:116593. [61] FILARDO G, PERDISA F, GELINSKY M, et al. Novel alginate biphasic scaffold for osteochondral regeneration: an in vivo evaluation in rabbit and sheep models. J Mater Sci Mater Med. 2018;29(6):74. [62] ÖZTÜRK E, STAUBER T, LEVINSON C, et al. Tyrosinase-crosslinked, tissue adhesive and biomimetic alginate sulfate hydrogels for cartilage repair. Biomed Mater. 2020;15(4):045019. [63] ARJMANDI M, RAMEZANI M. Mechanical and tribological assessment of silica nanoparticle-alginate-polyacrylamide nanocomposite hydrogels as a cartilage replacement. J Mech Behav Biomed Mater. 2019;95:196-204. [64] YUAN H, ZHENG X, LIU W, et al. A novel bovine serum albumin and sodium alginate hydrogel scaffold doped with hydroxyapatite nanowires for cartilage defects repair. Colloids Surf B Biointerfaces. 2020;192:111041. [65] SAYGILI E, KAYA E, ILHAN-AYISIGI E, et al. An alginate-poly(acrylamide) hydrogel with TGF-β3 loaded nanoparticles for cartilage repair: Biodegradability, biocompatibility and protein adsorption. Int J Biol Macromol. 2021;172:381-393. [66] XU L, MA F, HUANG J, et al. Metformin Hydrochloride Encapsulation by Alginate Strontium Hydrogel for Cartilage Regeneration by Reliving Cellular Senescence. Biomacromolecules. 2021;22(2):671-680. [67] ALMQVIST KF, DHOLLANDER AA, VERDONK PC, et al. Treatment of cartilage defects in the knee using alginate beads containing human mature allogenic chondrocytes. Am J Sports Med. 2009;37(10):1920-1929. [68] DHOLLANDER AA, HUYSSE WC, VERDONK PC, et al. MRI evaluation of a new scaffold-based allogenic chondrocyte implantation for cartilage repair. Eur J Radiol. 2010;75(1):72-81. [69] ZARRINTAJ P, KHODADADI YAZDI M, YOUSSEFI AZARFAM M, et al. Injectable Cell-Laden Hydrogels for Tissue Engineering: Recent Advances and Future Opportunities. Tissue Eng Part A. 2021;27(11-12):821-843. [70] JIN R, MOREIRA TEIXEIRA LS, KROUWELS A, et al. Synthesis and characterization of hyaluronic acid-poly(ethylene glycol) hydrogels via Michael addition: An injectable biomaterial for cartilage repair. Acta Biomater. 2010;6(6):1968-1977. [71] BORGES FTP, PAPAVASILIOU G, TEYMOUR F. Characterizing the Molecular Architecture of Hydrogels and Crosslinked Polymer Networks beyond Flory-Rehner-I. Theory. Biomacromolecules. 2020;21(12):5104-5118. [72] WANG J, ZHANG F, TSANG WP, et al. Fabrication of injectable high strength hydrogel based on 4-arm star PEG for cartilage tissue engineering. Biomaterials. 2017;120:11-21. [73] NEUMANN AJ, QUINN T, BRYANT SJ. Nondestructive evaluation of a new hydrolytically degradable and photo-clickable PEG hydrogel for cartilage tissue engineering. Acta Biomater. 2016;39:1-11. [74] ZHAO X, PAPADOPOULOS A, IBUSUKI S, et al. Articular cartilage generation applying PEG-LA-DM/PEGDM copolymer hydrogels. BMC Musculoskelet Disord. 2016;17:245. [75] FU N, LIAO J, LIN S, et al. PCL-PEG-PCL film promotes cartilage regeneration in vivo. Cell Prolif. 2016;49(6):729-739. [76] KO CY, KU KL, YANG SR, et al. In vitro and in vivo co-culture of chondrocytes and bone marrow stem cells in photocrosslinked PCL-PEG-PCL hydrogels enhances cartilage formation. J Tissue Eng Regen Med. 2016;10(10):E485-e496. [77] ZHANG Y, CAO Y, ZHANG L, et al. Fabrication of an injectable BMSC-laden double network hydrogel based on silk fibroin/PEG for cartilage repair. J Mater Chem B. 2020;8(27):5845-5848. [78] KIM JS, CHOI J, KI CS, et al. 3D Silk Fiber Construct Embedded Dual-Layer PEG Hydrogel for Articular Cartilage Repair - In vitro Assessment. Front Bioeng Biotechnol. 2021;9:653509. [79] ZHANG J, WANG J, ZHANG H, et al. Macroporous interpenetrating network of polyethylene glycol (PEG) and gelatin for cartilage regeneration. Biomed Mater. 2016;11(3):035014. [80] MALINAUSKAS M, JANKAUSKAITE L, AUKSTIKALNE L, et al. Cartilage regeneration using improved surface electrospun bilayer polycaprolactone scaffolds loaded with transforming growth factor-beta 3 and rabbit muscle-derived stem cells. Front Bioeng Biotechnol. 2022;10:971294. [81] KIM S, GWON Y, PARK S, et al. Synergistic effects of gelatin and nanotopographical patterns on biomedical PCL patches for enhanced mechanical and adhesion properties. J Mech Behav Biomed Mater. 2021; 114:104167. [82] VENUGOPAL E, SAHANAND KS, BHATTACHARYYA A, et al. Electrospun PCL nanofibers blended with Wattakaka volubilis active phytochemicals for bone and cartilage tissue engineering. Nanomedicine. 2019;21:102044. [83] MARTÍNEZ-MORENO D, JIMÉNEZ G, CHOCARRO-WRONA C, et al. Pore geometry influences growth and cell adhesion of infrapatellar mesenchymal stem cells in biofabricated 3D thermoplastic scaffolds useful for cartilage tissue engineering. Mater Sci Eng C Mater Biol Appl. 2021;122:111933. [84] OLUBAMIJI AD, IZADIFAR Z, SI JL, et al. Modulating mechanical behaviour of 3D-printed cartilage-mimetic PCL scaffolds: influence of molecular weight and pore geometry. Biofabrication. 2016;8(2):025020. [85] DI LUCA A, SZLAZAK K, LORENZO-MOLDERO I, et al. Influencing chondrogenic differentiation of human mesenchymal stromal cells in scaffolds displaying a structural gradient in pore size. Acta Biomater. 2016;36:210-219. [86] SUN Y, WU Q, ZHANG Y, et al. 3D-bioprinted gradient-structured scaffold generates anisotropic cartilage with vascularization by pore-size-dependent activation of HIF1α/FAK signaling axis. Nanomedicine. 2021;37:102426. [87] SILVA JC, UDANGAWA RN, CHEN J, et al. Kartogenin-loaded coaxial PGS/PCL aligned nanofibers for cartilage tissue engineering. Mater Sci Eng C Mater Biol Appl. 2020;107:110291. [88] DABASINSKAITE L, KRUGLY E, BANIUKAITIENE O, et al. The Effect of Ozone Treatment on the Physicochemical Properties and Biocompatibility of Electrospun Poly(ε)caprolactone Scaffolds. Pharmaceutics. 2021;13(8):1288. [89] LI J, YAO Q, XU Y, et al. Lithium Chloride-Releasing 3D Printed Scaffold for Enhanced Cartilage Regeneration. Med Sci Monit. 2019;25:4041-4050. [90] WEI P, XU Y, GU Y, et al. IGF-1-releasing PLGA nanoparticles modified 3D printed PCL scaffolds for cartilage tissue engineering. Drug Deliv. 2020; 27(1):1106-1114. [91] JIANG T, HENG S, HUANG X, et al. Biomimetic Poly(Poly(ε-caprolactone)-Polytetrahydrofuran urethane) Based Nanofibers Enhanced Chondrogenic Differentiation and Cartilage Regeneration. J Biomed Nanotechnol. 2019; 15(5):1005-1017. [92] JIANG T, KAI D, LIU S, et al. Mechanically cartilage-mimicking poly(PCL-PTHF urethane)/collagen nanofibers induce chondrogenesis by blocking NF-kappa B signaling pathway. Biomaterials. 2018;178:281-292. [93] LI Z, LIU P, YANG T, et al. Composite poly(l-lactic-acid)/silk fibroin scaffold prepared by electrospinning promotes chondrogenesis for cartilage tissue engineering. J Biomater Appl. 2016;30(10):1552-1565. [94] DING J, CHEN B, LV T, et al. Bone Marrow Mesenchymal Stem Cell-Based Engineered Cartilage Ameliorates Polyglycolic Acid/Polylactic Acid Scaffold-Induced Inflammation Through M2 Polarization of Macrophages in a Pig Model. Stem Cells Transl Med. 2016;5(8):1079-1089. [95] WAYNE JS, MCDOWELL CL, SHIELDS KJ, et al. In vivo response of polylactic acid-alginate scaffolds and bone marrow-derived cells for cartilage tissue engineering. Tissue Eng. 2005;11(5-6):953-963. [96] LIU Q, WANG J, CHEN Y, et al. Suppressing mesenchymal stem cell hypertrophy and endochondral ossification in 3D cartilage regeneration with nanofibrous poly(l-lactic acid) scaffold and matrilin-3. Acta Biomater. 2018;76:29-38. [97] MING L, ZHIPENG Y, FEI Y, et al. Microfluidic-based screening of resveratrol and drug-loading PLA/Gelatine nano-scaffold for the repair of cartilage defect. Artif Cells Nanomed Biotechnol. 2018;46(sup1):336-346. [98] LI T, LIU B, JIANG Y, et al. L-polylactic acid porous microspheres enhance the mechanical properties and in vivo stability of degummed silk/silk fibroin/gelatin scaffold. Biomed Mater. 2020;16(1):015025. |
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1.1.7 检索策略 以 PubMed 数据库检索策略为例,见图1。
1.1.8 检索文献量 初步检索到文献367篇。
1.3 文献质量评估与数据提取 共检索到中英文文献共367篇,选择与文章内容相关性大并具有价值的文章进行分析讨论,排除与研究目的相关性差及内容陈旧、重复的文献269篇,最终按入选标准严格筛选后最终纳入98篇符合标准的英文文献进行综述,见图2。
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文题释义:
软骨组织工程:是指将种子细胞植入模拟软骨细胞外基质结构的凝胶/支架中,并将支架材料移植在缺损软骨部位,在生长因子作用下使种子细胞增殖分化、合成并分泌细胞外基质,以达到软骨修复的目的。软骨组织损伤修复是临床中一大难点,组织工程技术利用支架/凝胶模拟天然软骨组织细胞外基质作为种子细胞的培养环境,并提供物理力学刺激和生化因子刺激,可诱导促进软骨组织修复。文章从软骨组织工程修复材料为切入点,综述胶原、明胶、丝素蛋白和聚乙二醇等多种天然与合成材料在软骨组织工程中的作用及应用研究,涉及多种材料的改性、材料复合、药物负载及相关临床试验研究,为软骨组织工程材料的后续研究和应用提供参考,也为支架的研发和临床转化提供新的思路。
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