中国组织工程研究 ›› 2020, Vol. 24 ›› Issue (31): 4941-4947.doi: 10.3969/j.issn.2095-4344.2137

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

煅烧骨/壳聚糖复合材料促进SD大鼠骨髓间充质干细胞的成骨分化

廖  健1,黄晓林2,周  倩1,霍  花1,齐昱晗1,伍  超1,石前会1,杨童景1,廖运茂3,梁  星3    

  1. 1贵州医科大学口腔医学院/附属口腔医院,贵州省贵阳市  550004;2中山市人民医院口腔种植科,广东省中山市  528403;3四川大学华西口腔医学院,四川省成都市  610041

  • 收稿日期:2020-02-27 修回日期:2020-03-05 接受日期:2020-04-15 出版日期:2020-11-08 发布日期:2020-09-03
  • 通讯作者: 梁星,教授,博士研究生导师,四川大学华西口腔医学院,四川省成都市 610041
  • 作者简介:廖健,男,1978年生,贵州省石阡县人,仡佬族,四川大学华西口腔医学院毕业,博士,副教授,副主任医师,硕士生导师,主要从事口腔修复与种植的基础、临床研究。
  • 基金资助:
    国家自然科学基金(81660179);贵州省自然科学基金 ([2016]1124);贵州省自然科学基金联合基金(LH[2016]7257)

Calcined bone/chitosan composite promotes osteogenic differentiation of bone marrow mesenchymal stem cells in Sprague-Dawley rats  

Liao Jian1, Huang Xiaolin2, Zhou Qian1, Huo Hua1, Qi Yuhan1, Wu Chao1, Shi Qianhui1, Yang Tongjing1,Liao Yunmao3, Liang Xing3   

  1. 1School/Hospital of Stomatology, Guizhou Medical University, Guiyang 550004, Guizhou Province, China; 2Department of Dental Implant, Zhongshan People’s Hospital, Zhongshan 528403, Guangdong Province, China; 3West China School of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China

  • Received:2020-02-27 Revised:2020-03-05 Accepted:2020-04-15 Online:2020-11-08 Published:2020-09-03
  • Contact: Liang Xing, Professor, Doctoral supervisor, West China School of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • About author:Liao Jian, MD, Associate professor, Associate chief physician, Master’s supervisor, School/Hospital of Stomatology, Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81660179 ; the Natural Science Foundation of Guizhou Province, No. [2016]1124 ; the Joint Fund of Natural Science Foundation of Guizhou Province, No. LH[2016]7257

摘要:

文题释义:

锻烧骨:是经高温锻烧处理动物骨所获得的无机材料,主要成分是羟基磷灰石,钙磷比接近于人骨,与人工合成羟基磷灰石相比,煅烧骨保留了天然骨的孔隙率、孔径大小、孔隙贯通等结构特点,而且制作成本低、方法简单。然而,锻烧骨由于高温处理使其失去了有机相的胶原,其力学强度下降,脆性增加,多数情况下只能作为充填材料用于拔牙窝位点保存,对于牙槽骨骨量严重不足的种植病例其应用受到限制。

壳聚糖:是一类重要的碱性天然多糖,无毒、具有良好的生物相容性,生物可降解,能够增强前体骨细胞的分化,促进骨的形成。然而,单一组分的壳聚糖由于缺乏骨组织的无机结构,没有骨键合的生物活性,不能直接用于骨组织修复,所以通常被用作辅助材料与其他骨移植材料复合,从而使两种材料达到优势互补的生物性能,更好地满足骨移植材料的要求。

背景:课题组前期研究已经证实煅烧骨/壳聚糖复合材料的微观结构与自然骨组织相似,安全无毒,抗压强度良好,并且能够促进成骨细胞的增殖及黏附,因此继续研究其对骨髓间充质干细胞成骨分化的作用具有重要意义。

目的:探讨煅烧骨/壳聚糖复合材料对骨髓间充质干细胞成骨分化的影响。

方法:将煅烧骨/壳聚糖复合材料与SD大鼠骨髓间充质干细胞体外共培养并进行成骨诱导,成骨诱导123周时,采用实时荧光定量PCR检测成骨分化相关基因的表达。

结果与结论:煅烧骨/壳聚糖复合材料能促进骨髓间充质干细胞的增殖黏附,并促进成骨相关基因碱性磷酸酶、型胶原、骨钙素的表达,具有诱导骨髓间充质干细胞成骨分化的作用。

ORCID: 0000-0003-3519-4485(廖健)

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


关键词: 干细胞, 间充质干细胞,  骨髓,  煅烧骨,  壳聚糖,  骨,  实验,  基因

Abstract:

BACKGROUND: Our previous research has confirmed that the microstructure of calcined bone/chitosan composite is similar to that of natural bone tissue, safe and non-toxic, with good compressive strength, and can promote the proliferation and adhesion of osteoblasts. Therefore, it is necessary to investigate the effect of the composite on the osteogenic differentiation of bone marrow mesenchymal stem cells.

OBJECTIVE: To investigate the effect of calcined bone/chitosan composite on osteogenic differentiation of bone marrow mesenchymal stem cells.

METHODS: The calcined bone/chitosan composite was co-cultured with bone marrow mesenchymal stem cells of Sprague-Dawley rats for osteogenic induction in vitro. At 1, 2 and 3 weeks after osteogenic induction, the expression of genes related to osteogenic differentiation was detected by real-time fluorescence quantitative PCR.

RESULTS AND CONCLUSION: The calcined bone/chitosan composites can promote the proliferation and adhesion of bone marrow mesenchymal stem cells and promote the expression of osteogenic genes alkaline phosphatase, type I collagen, and osteocalcin, which can induce the osteogenic differentiation of bone marrow mesenchymal stem cells. 

Key words: stem cells,  mesenchymal stem cells,  bone marrow,  calcined bone,  chitosan,  bone,  experiment,  gene

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