Table of Content

03 June 2012, Volume 16 Issue 23 Previous Issue    Next Issue

For Selected:

Download Citations EndNote Reference Manager ProCite BibTeX RefWorks

Toggle Thumbnails

Select

Expression of annexin A1 in rabbit bone marrow mesenchymal stem cells during their early differentiation into osteoblasts and adipocytes in vitro Pan Xin-yuan, Liang Bo-wei, Zhao Jin-min, Yin Guo-qian 2012, 16 (23):  4181-4186.  doi: 10.3969/j.issn.1673-8225.2012.23.001 Abstract ( 186 )   PDF (676KB) ( 328 )   Save BACKGROUND: Studies have reported different views on the expression of annexin A1 (ANX A1) in bone marrow mesenchymal stem cells (BMSCs) during their differentiation. OBJECTIVE: To investigate the expression of ANX A1 in rabbit BMSCs during their differentiation into osteoblasts and adipocytes in vitro. METHODS: BMSCs were isolated by whole bone marrow adherence method, and then the cells were cultured in media with osteogenic inducer, adipogenic inducer and non-inducer respectively. RESULTS AND CONCLUSION: The expression of ANX A1 mRNA was obviously decreased during the cell differentiation into osteoblasts compared with those without differentiation (P < 0.01). In contrast, the expression of ANX A1 mRNA was obviously increased in BMSCs cultured in medium with adipogenic inducer (P < 0.01). Osteogenic inducer could inhibit the growth of BMSCs to achicve an increase in apoptosis (P < 0.01), while the adipogenic inducer had little effects on cell growth and apoptosis (P > 0.05). ANX A1 may play an important role in regulation of BMSCs differentiation into adipocytes in vitro without the role of inducer. However, the association with osteogenic differentiation is uncertain. Related Articles | Metrics

Select

Bone marrow-derived mesenchymal stem cell transplantation increased expression of neural cell adhesion molecules in a rat model of ischemic brain injury Peng Yu, Zhang Qi-mei, You Hui, Zhang Ying, Zhuang Wei-hua, Zhang Qiang, Zhou Jing-hua 2012, 16 (23):  4187-4192.  doi: 10.3969/j.issn.1673-8225.2012.23.002 Abstract ( 212 )   PDF (567KB) ( 305 )   Save BACKGROUND: Studies have demonstrated that transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) improves neurofunctional recovery and the underlying mechanism may be related to upregulated expression of neural cell adhesion molecules. OBJECTIVE: To investigate the effects of BMSC transplantation on the expression of neural cell adhesion molecule after ischemic brain injury in rats. METHODS: Sprague-Dawley rats were randomly divided into a sham-operated group and a middle cerebral artery occlusion (MCAO) group. A permanent focal cerebral ischemia model was established using modified Longa’s method. The MCAO groups were randomly subdivided into a BMSC group (transplantation of 5×105 allogenic BMSCs via the left lateral ventricle) and a control group (phosphate buffered saline injection). The sham-operated group received neither MCAO nor BMSC transplantation. RESULTS AND CONCLUSION: At 7 and 14 days following transplantation, neural cell adhesion molecule expression surrounding the infarct foci was significantly higher in the BMSC group and control group (P < 0.01), and the expression in the BMSC group was significantly higher than in the control group (P < 0.05). At 14 days following transplantation, neurological severity score in the BMSC group was significantly lower than in the control group (P < 0.05). BMSC transplantation promoted neurofunctional recovery by upregulating neural cell adhesion molecule expression in the marginal zone of cerebral infarct focus of a rat model of focal cerebral ischemia. Related Articles | Metrics

Select

Effect of matrix metalloproteinase-1 on myogenic differentiation of mouse bone marrow-derived mesenchymal stem cells in vitro Zheng Zhen-yang, Zhang Wei-xi, Leng Yan, Zhou Chen, Ma Zhen-yu, Zhong Zhi-gang 2012, 16 (23):  4193-4198.  doi: 10.3969/j.issn.1673-8225.2012.23.003 Abstract ( 279 )   PDF (612KB) ( 351 )   Save BACKGROUND: The myogenic differentiation potential of bone marrow-derived mesenchymal stem cells (MSCs) in vitro from mice is low. OBJECTIVE: To explore the effect of matrix metalloproteinase-1 (MMP-1) on myogenic differentiation of MSCs in vitro from mice. METHODS: After MSCs were isolated and cultured by differential adhesion, as well as identified by cell surface markers and multi-lineage differentiation potential, the cells were assigned to 4 groups according to various MMP-1 concentration gradients (10 μg/L group, 1 μg/L group, 0.1 μg/L group and control group). The mRNA expressions of MyoD and Desmin were detected by real-time quantitative PCR, and the expression of protein Desmin was tested by Western Blotting. RESULTS AND CONCLUSION: MSCs obtained from differential adhesion were of morphological homogenicity, and they were CD29+, Sca-1+, CD45- and CD34-. Additionally, MSCs were capable of multi-lineage differentiation. The mRNA expression of Desmin and MyoD as well as the expression of protein Desmin were up regulated via MMP-1 inducement. Moreover, the up-regulation degree of mRNA and protein expression was concentration-dependent. These suggest that MMP-1 can promote MSCs to differentiate into muscle cells in vitro. Related Articles | Metrics

Select

Effects of platelet-rich plasma gel on the proliferation and osteogenic activity of rabbit bone marrow mesenchymal stem cells Zhang Lin-pu, Dai Xiao-hua, Wang Guan-hua, Li Yan-ni, Lian Xiao-li, Yao Hui 2012, 16 (23):  4199-4203.  doi: 10.3969/j.issn.1673-8225.2012.23.004 Abstract ( 263 )   PDF (409KB) ( 356 )   Save BACKGROUND: Platelet-rich plasma (PRP) contains several kinds of growth factors related to bone repair, and can form fibrin meshwork scaffold after agglomerated by activation, which is beneficial to cells adhesion and promotes bone regeneration. OBJECTIVE: To evaluate the proliferation and osteogenic activity of rabbit bone marrow mesenchymal stem cells (rBMSCs) in platelet-rich plasma gel in vitro. METHODS: rBMSCs were isolated from bone marrow of limb long bone of 5-day neonatal rabbits by adhesion culture. PRP was obtained from adult rabbit peripheral venous blood. Experiment samples were divided into three groups: PRP plus rBMSCs (PRP/rBMSCs) group, PRP plus α-MEM medium (PRP/α-MEM) group and rBMSCs group. RESULTS AND CONCLUSION: The difference of lactate dehydrogenase activity among three groups was significant (P < 0.05), and the cell proliferation in PRP/rBMSCs group was obviously better than that in the other two groups. The PRP/rBMSCs gel was complete, uniform and semitransparent. It had many cells which were anomalistic polygon with long synapse distributing in gel. Tetracycline labeled flavo-green fluorescence brightened dots and nodes were discovered in PRP/rBMSCs gel after 2 and 3 weeks of culture using fluorescence microscope. Calcify nodes were formed in the PRP/rBMSCs gel. The number and size of fluorescence brightened dots and nodes founded in the third week after culture were a little more and bigger than those found in the second week. There were no fluorescence brightened dots and nodes in PRP/α-MEM gel. It indicates that rBMSCs combined with PRP gel can promote the proliferation and osteogenic activity of rBMSCs. Related Articles | Metrics

Select

Effects of human bone marrow mesenchymal stem cells and their conditioned medium on the secretary function of allogenous peripheral blood T lymphocytes Bai Hai, Wu Bing, Wang Cun-bang, Ma Xiao-hui, Si Zhi-gang 2012, 16 (23):  4204-4208.  doi: 10.3969/j.issn.1673-8225.2012.23.005 Abstract ( 250 )   PDF (403KB) ( 386 )   Save BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) are characterized by supporting hematopoiesis, pluripotential differentiation and immunoregulation. The immunoregulatory effects are of potential importance in prevention and treatment of graft-versus-host disease (GVHD) after transplantation of allogenous hematopoietic stem cells, but the mechanism of immuno-regulation remains unknown. OBJECTIVE: To investigate the immunoregulatory effects of human BMSCs and their conditioned medium on the secretary function of allogenous peripheral blood T lymphocytes in vitro. METHODS: BMSCs were isolated from human bone marrow and purified by repeated adherence. Allogenous peripheral blood T lymphocytes were harvested using nylon column. The effects of BMSCs and their conditioned medium on the proliferation of phytohemagglutinin-primed T lymphocytes were observed. Interferon-γ and interleukin 4 levels were detected by ELISA. RESULTS AND CONCLUSION: BMSCs and their conditioned medium inhibited the proliferation of phytohemagglutinin-primed T lymphocytes in a dose- and concentration-dependent manner. ELISA results showed that BMSCs and their conditioned medium inhibited the secretion of interferon-γ by T lymphocytes. BMSCs could promote the secretion of interleukin-4, but their conditioned medium did not influence the secretion of interleukin-4. These findings suggest that BMSCs and their conditioned medium inhibit the proliferation of allogenous peripheral blood T lymphocytes, and the inhibitory effects may be related to a fact that BMSCs and their conditioned medium influence T lymphocytes to secrete interferon-γ and interleukin-4. Related Articles | Metrics

Select

Effect of intermittent treatment with parathyroid hormone on human bone marrow stromal stem cells Yin Xiao-xue, Zhou Fang, Zhang Zhi-shan, Song Chun-li 2012, 16 (23):  4209-4212.  doi: 10.3969/j.issn.1673-8225.2012.23.006 Abstract ( 191 )   PDF (332KB) ( 341 )   Save BACKGROUND: Continuous and intermittent application of parathyroid hormone at different doses can cause different effects on bone. OBJECTIVE: To observe the effect of intermittent treatment with different doses of parathyroid hormone on the proliferation and differentiation of human bone marrow stromal stem cells. METHODS: Human bone marrow stromal stem cells were cultured in vitro and divided into blank control group and 5, 20 and 50 nmo/L parathyroid hormone group, and the parathyroid hormone groups were treated for 1 hour per day. MTT and alkaline phosphatase activities were used to detect the proliferation and differentiation of human bone marrow stromal stem cells to osteoblasts. RESULTS AND CONCLUSION: 50 nmol/L of parathyroid hormone treated for 1 hour per day could greatly promote the proliferation of human bone marrow stromal stem cells and could increase the alkaline phosphatase activities, and the alkaline phosphatase activities in 50 nmo/L parathyroid hormone group were higher than other three groups. Intermittent treatment with 50 nmol/L parathyroid hormone can promote the proliferation and differentiation of human bone marrow stromal stem cells. Related Articles | Metrics

Select

Co-culture of umbilical cord blood-derived monocytes and bone marrow mesenchymal stem cells Wang Lei, Song Jie, Kuang Tao 2012, 16 (23):  4213-4216.  doi: 10.3969/j.issn.1673-8225.2012.23.007 Abstract ( 198 )   PDF (462KB) ( 273 )   Save BACKGROUND: Bone marrow mesenchymal stem cells support hematopoiesis in vivo and in vitro and play an important role in regulation of hematopoiesis. OBJECTIVE: To investigate the biological characteristics and multi-directional differentiation capability of bone marrow mesenchymal stem cells and their ability to support hematopoiesis in vivo and in vitro. METHODS: Bone marrow monocytes were isolated by density gradient culture method. Bone marrow mesenchymal stem cell phenotype was detected by flow cytometry. Umbilical cord monocytes were inoculated onto bone marrow mesenchymal stem cell trophoblast for co-culture. Granulocyte-monocyte colony forming units were counted. RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells showed typical fibroblast-like cell morphology and strongly expressed CD44 and CD29, but they were negative for CD34 and CD106. Bone marrow mesenchymal stem cells could promote the proliferation of umbilical cord monocytes to form hematopoietic progenitor colonies. These findings suggest that bone marrow mesenchymal stem cells support hematopoiesis. Related Articles | Metrics

Select

Differentiation of human umbilical cord mesenchymal stem cells into germ cells induced by embryoid bodies Lü Pin, He Ling, Chai Hui-xia 2012, 16 (23):  4217-4221.  doi: 10.3969/j.issn.1673-8225.2012.23.008 Abstract ( 268 )   PDF (520KB) ( 517 )   Save BACKGROUND: Whether umbilical mesenchymal stem cells (UC-MSCs) can be induced into oocytes under in vitro and in vivo microenvironment is very important for female reproductive maintenance and oocyte regeneration. OBJECTIVE: To further testify the effects of embryonic bodies (EB) under in vitro microenvironment on the differentiation of UC-MSCs into germ cells. METHODS: UC-MSCs were cultured in hanging drop to form EB, which were co-cultured with human ovarian granulosa cells or mouse ovarian granulosa cells, or were cultured in follicular fluid to induce differentiation of UC-MSCs into primary germ cells in vitro. RESULTS AND CONCLUSION: The EB were successfully prepared when UC-MSCs were cultured in hanging drop. Flow cytometry results: SSEA-1+ cells accounted for 15.61% after 5 days of EB formation. The results of immunohistochemistry: EB of 5 days were co-cultured with human ovarian granulosa cells or mouse ovarian granulosa cells for 10 days, germ-line markers of synaptonemal complex protein-3 and growth differentiation factor-9 positively expressed, while these two markers did not express in granule cells or EB cultured in follicular fluid. EB can be formed when UC-MSCs are cultured in hanging drop in vitro. Germ-line markers will express in EB when they are co-cultured with human ovarian granulosa cells or mouse ovarian granulosa cells. Related Articles | Metrics

Select

Fluorescent dye CM-DiI labeled rat adipose derived stem cells Song Qi-bin, Liu Xiao-yan, Cao Hui-juan, Yu Dong-mei, Huang Xin-xin 2012, 16 (23):  4222-4226.  doi: 10.3969/j.issn.1673-8225.2012.23.009 Abstract ( 212 )   PDF (513KB) ( 1004 )   Save BACKGROUND: Dil derivative CM-Dil easily insets, disperses and stably binds to the whole cell membrane because of water-solubility and chloromethylated active sulfydryl, which leads to rapid, even and long-lasting staining. OBJECTIVE: To further validate the feasibility of fluorescent dye CM-Dil in labeling rat adipose derived stem cells. METHODS: Rat inguinal fat tissue was resected and adipose derived stem cells were isolated in vitro by collagenase digestion. Passage 3 cells were divided into a control group and an experimental group. The experimental group cells were labeled with 4 mg/L CM-Dil. At 6, 12, 24 and 48 hours, CM-Dil-labeled adipose derived stem cells were traced in vivo. RESULTS AND CONCLUSION: Under fluorescent microscope, CM-Dil labeled cells showed cytoplasm and cell membrane with red fluorescence, CM-Dil labeled adipose derived stem cells exhibited a shuttle-shaped normal appearance. CM-Dil positive rate was 100%. In the early period, the cells were fluorescent ring-shaped, and 48 hours later, fluorescent granules increased and fluorescence intensity enhanced. No fluorescence was found in cell nucleus. There was no significant difference in cell morphology, content of lactic dehydrogenase in supernatant and MTT value between experimental group and control group (P > 0.05). At 4 hours after cell transplantation, cells with red fluorescence could be observed in the heart and lung tissue. CM-DiI can effectively label adipose derived stem cells cultured in vitro and express stably in cells. CM-Dil-labeled cells have good morphology and no toxicity to living cells. Related Articles | Metrics

Select

In vitro induced differentiation of adipose stem cells into dopaminergic neurons Gao Hua, Yao Ya-ni, Wang Yu-ling, Yang Xin-ling 2012, 16 (23):  4227-4231.  doi: 10.3969/j.issn.1673-8225.2012.23.010 Abstract ( 280 )   PDF (478KB) ( 315 )   Save BACKGROUND: Adipose stem cells are considered as the seed cells for the treatment of Parkinson’s disease due to their characteristics such as rich sources, simple drawing, easy to purify and amplify, autologous transplantation and multipotential differentiation. OBJECTIVE: To observe the differentiation of rat adipose stem cells into dopaminergic neurons and their secreted function. METHODS: The adipose stem cells of Sprague -Dawley rats were obtained by collagenase digestion. In the experimental group, the cells were differentiated into dopaminergic neurons co-induced by basic fibroblast growth factor, epidermal growth factor and ascorbic acid after cultured to the third passage in vitro. In the control group, the cells were simply added with 5% serum. RESULTS AND CONCLUSION: Tyrosine hydroxylase expression in the experimental group was examined by immunofluorescence and immunohistochemisty after induced for 3 days. The proportion of dopaminergic neurons in experimental group was significantly higher than that in the control group (P < 0.01). The level of dopaminergic neurons in cell supernatant was detected by ELISA assay after successively induced for 3 days; the concentration of dopaminergic neurons was increased at the first and second days after induction, and decreased at the third day. The results showed that the adipose stem cells could be differentiated into dopaminergic neurons. Related Articles | Metrics

Select

Differentiation of adipose-derived stem cells into islet β-like cells in vitro Yang Guang, Zhao Zhan-zhi, Zhu Xin-bing, Hu Song 2012, 16 (23):  4232-4236.  doi: 10.3969/j.issn.1673-8225.2012.23.011 Abstract ( 241 )   PDF (550KB) ( 465 )   Save BACKGROUND: Islet β-like cells can be induce-differentiated by embryonic stem cells, pancreatic stem cells, hepatic oval cells, intestinal stem cells and bone marrow mesenchymal stem cells in vivo. Then whether adipose-derived stem cells can be differentiate into islet β-like cells remains pooly understod? OBJECTIVE: To investigate the feasibility of adipose-derived stem cells to differentiate into islet β-like cells. METHODS: Adipose-derived stem cells were isolated and cultured from rabbit liposuction, and after two generations passage, the adipose-derived stem cells were differentiated into islet β-like cells induced by high glucose, basic fibroblast growth factorand nicotamine in culture medium. RESULTS AND CONCLUSION: After the passage 2 adipose-derived stem cells were induced by high glucose, the adipose-derived stem cells formed cell clusters and dithizone-stained cell clusters in flask were observed. Immunocytochemistry also confirmed that these cell clusters were positive for insulin. Adipose-derived stem cells can differentiate into islet β-cells in vitro. These islet β-cells can synthesize and store insulin. Related Articles | Metrics

Select

Effects of stromal cell-derived factor-1 and its receptor CXCR4 on the migration of endogenous neural stem cells Dai Xiao-sen, Ding Peng, Dong Yue, Mu Lin-jie, Wang Wei-min, Wang Chong-qian, Wang Jin-kun 2012, 16 (23):  4237-4241.  doi: 10.3969/j.issn.1673-8225.2012.23.012 Abstract ( 250 )   PDF (534KB) ( 391 )   Save BACKGROUND: Neural stem cells own the characteristics of migration in vitro. However, the mechanisms underlying the migration of these cells remain unclear. OBJECTIVE: To investigate the effects of stromal cell-derived factor-1 and its receptor CXCR4 on the migration of neural stem cells in vivo. METHODS: The brain injured rat model was established with cortical micro-injection of lipopolysaccharide. At 3, 5, and 7 days after modeling, the brain tissue was harvested. Immunohistochemical staining of frozen section was used to detect the expression of stromal cell-derived factor-1 and nestin-positive cells in different times and phages. RESULTS AND CONCLUSION: Immunohistochemical staining showed that the expression of stromal cell-derived factor-1 was in a time-dependent manner. Nestin-positive cells extended and migrated outward significantly and pointed to the lipopolysaccharide injection area. Stromal cell-derived factor-1 can induce the endogenous neural stem cells to move towards lipopolysaccharide injection area in vivo. Related Articles | Metrics

Select

Transplantation of human bone morphogenetic protein-2 gene transfected autogenous bone marrow mesenchymal stem cells promotes bone regeneration in rabbit mandibular distraction osteogenesis: A histomorphological study Huang Xuan-ping, Zhou Nuo, Jiang Xian-fang, Song Ji-zhuan, Li Hua, Xie Qing-tiao 2012, 16 (23):  4242-4246.  doi: 10.3969/j.issn.1673-8225.2012.23.013 Abstract ( 225 )   PDF (535KB) ( 456 )   Save BACKGROUND: How to promote new bone formation and regeneration quality, shorten hospitalization and treatment times, and decrease related complications has recently been the edge-cutting focus in the field. OBJECTIVE: To investigate the effect of human bone morphogenetic protein-2 gene transfected autogenous bone marrow mesenchymal stem cells transplantation on promoting rabbit bone regeneration in mandibular distraction osteogenesis by histomorphological method. METHODS: Thirty-six New Zealand white rabbits were randomly divided into three groups with 12 rabbits in each. All the objects were prepared into distraction osteogenesis surgical models on the right mandible. On the 2nd day of consolidation period, rats in the experimental group, control group and blank group were injected with the same amount of human bone morphogenetic protein-2 gene transfected autogenous bone marrow mesenchymal stem cells, autogenous bone marrow mesenchymal stem cells and physiological saline at distraction gap, respectively. RESULTS AND CONCLUSION: The quality of bone trabecula of the experimental group was better than that of the control group and blank group at 2 and 6 weeks of consolidation period. The transplantation of human bone morphogenetic protein-2 gene transfected autogenous bone marrow mesenchymal stem cells can effectively promote bone regeneration in rabbit mandibular distraction osteogenesis. Related Articles | Metrics

Select

Differentiation of bone marrow-derived neural stem cells transfected by nuclear receptor-related factor 1 gene into dopaminergic neurons in vitro Liang Wei, Xu Zhi-en, Ke Jun-long, Chen Jing-juan, Li Hua 2012, 16 (23):  4247-4252.  doi: 10.3969/j.issn.1673-8225.2012.23.014 Abstract ( 272 )   PDF (505KB) ( 347 )   Save BACKGROUND: Few reports have addressed whether genetic engineering of bone marrow-derived neural stem cells transfected by nuclear receptor-related factor 1 (Nurr1) gene can enhance the differentiation into dopaminergic neurons. OBJECTIVE: To investigate the differentiation of bone marrow-derived neural stem cells transfected by Nurr1 gene into DA neurons in vitro. METHODS: Rat bone marrow mesenchymal stem cells were in vitro cultured and purified and then divided into four groups. The un-transfected bone marrow-derived mesenchymal stem cells were divided into control group and brain-derived neurotrophic factor (BDNF) group; the screened recombinant plasmid-positive neural stem cells were divided into Nurr1 group and Nurr1+BDNF group. RESULTS AND CONCLUSION: Plenty of Nurr1 in transfected bone marrow-derived mesenchymal stem cells were detected by RT-PCR after 4 days. Differentiation results showed that the mRNA expression of tyrosine hydroxylase in the cells of Nurr1+BDNF group was highest. The proportion of tyrosine hydroxylase positive cells in BDNF group, Nurr1 group and Nurr1+BDNF group was significantly higher than that in the control group after adherent differentiation of neural stem cells. The proportion of tyrosine hydroxylase-positive cells in the Nurr1+BDNF group was highest; the proportion was (52.44±15.9) %. The results indicate that the Nurr1 gene can promote bone marrow-derived mesenchymal stem cells to differentiate into dopaminergic neurons, and a large number of dopaminergic neurons are obtained after being induced by BDNF. Related Articles | Metrics

Select

Transforming growth factor beta 1 promotes the migration of mesenchymal stem cells via upregulation of Snail expression Zheng Li-fang, Mei Yuan-wu, Zhang Xiao-qiao, Zhang Hong 2012, 16 (23):  4253-4258.  doi: 10.3969/j.issn.1673-8225.2012.23.015 Abstract ( 259 )   PDF (357KB) ( 321 )   Save BACKGROUND: Transformation growth factor β1 (TGF-β1) can promote bone marrow mesenchymal stem cells (BMSCs) migration and proliferation, but the underlying mechanisms remain unclear. OBJECTIVE: To observe the invasiveness of TGF-β1 on BMSCs cultured in vitro, and to investigate regulatory effect on Snail and matrix metalloproteinase 2 (MMP-2) expression. METHODS: Rat BMSCs were isolated and cultured with density gradient centrifugalization and adherence method. The influence of different concentrations of TGF-β1 on the BMSC migration was detected using the modified Transwell chambers. Small interfering RNA for Snail gene was synthesized and transfected into BMSCs by liposomel before TGF-β1 was treated, and the expression of Snail and MMP-2 before and after transfection were measured by western blot assay. RESULTS AND CONCLUSION: The exogenous TGF-β1 can induce a dose-dependent increase in cell migration, which peaked at 2 μg/L. The expression levels of Snail mRNA and MMP-2 mRNA were significantly increased after 2 μg/L TGF-β1 treatment. Snail gene can effectively inhibit the expression of MMP-2 promoted by TGF-β1. Experimental findings indicate that TGF-β1 could increase the MMP-2 expression and then promote the BMSCs migration through the upregulation of the Snail expression. Related Articles | Metrics

Select

Whole genome binding sites of stem cell regulator nervous system polycomb 1 Li Hui, Liu Yuan, Gong Yan-hua 2012, 16 (23):  4259-4262.  doi: 10.3969/j.issn.1673-8225.2012.23.016 Abstract ( 329 )   PDF (346KB) ( 353 )   Save BACKGROUND: Polycomb group member nervous system polycomb 1 (NSPc1) is a transcription repressor which is highly expressed in the embryo nervous system. Recent research has shown that NSPc1 should be a new regulator of neural stem cell as well as its homologue Bmi1. OBJECTIVE: To identify the whole genome binding sites of stem cell regulator NSPc1 in the P19 neural differentiation model (mouse embryonal carcinoma cells). METHODS: Ex vivo expanded undifferentiated P19 cells, which have high expression of endogenous NSPc1 gene, were applied to chromatin immunoprecipitation (ChIP) analysis by using rabbit anti-NSPc1 polyclonal antibody. ChIP-on-chip, a high throughput method of screening the binding targets of NSPc1, was used within these P19 cells. Finally, the functional features of targets were analyzed with bio-informatics methods. RESULTS AND CONCLUSION: Nearly 1 280 NSPc1 target genes were screened out. Gene Ontology terms associated with "differentiation, development, transcription and its regulation, neurogenesis" were of significantly higher frequency with NSPc1 target genes than their random frequency across the entire genome. Whole genome binding sites analysis is able to indicate the target gene profile of NSPc1 in P19 neural differentiation model, which is very helpful to further investigate the downstream signaling pathway of the new stem cell regulator NSPc1. Related Articles | Metrics

Select

Angiogenesis of autologous bone marrow mononuclear cells by intramyocardial and intracoronary transplantation into ischemic myocardium of a swine model of acute myocardial infarction Sun Lin, Chu Tian-shu, Bai Wen-wei, Wang Li-qin, Zhang Ge, Ruan Zhi-min 2012, 16 (23):  4263-4266.  doi: 10.3969/j.issn.1673-8225.2012.23.017 Abstract ( 213 )   PDF (340KB) ( 381 )   Save BACKGROUND: Current evidence exists that intramyocardial transplantation of bone marrow mononuclear cells can promote angiogenesis and blood supply in ischemic myocardium. OBJECTIVE: To investigate the effects of intramyocardial and intracoronary transplantation of autologous bone marrow mononuclear cells into ischemic myocardium of a swine model of acute myocardial infarction. METHODS: Twenty-two swine models of acute myocardial infarction were randomly divided into four groups. In the intramyocardial transplantation group, immediately after induction of acute myocardial infarction, autologous bone marrow mononuclear cell suspension was injected into the ischemic myocardium. In the intramyocardial transplantation control group, autologous bone marrow mononuclear cell suspension was replaced by Hank’s balanced salt solution. In the intracoronary transplantation group, at 1 week after induction of acute myocardial infarction, autologous bone marrow mononuclear cell suspension was injected into the left coronary artery. In the intracoronary transplantation control group, autologous bone marrow mononuclear cell suspension was replaced by Hank’s balanced salt solution. RESULTS AND CONCLUSION: At 1 week after transplantation, there were no significant differences in serum levels of basic fibroblast growth factor and vascular endothelial growth factor between intramyocardial transplantation group and intracoronary transplantation group. The serum levels of basic fibroblast growth factor and vascular endothelial growth factor in intramyocardial transplantation group or intracoronary transplantation group were significantly higher than those in corresponding control group (P < 0.01). At 4 weeks after transplantation, there was no significant difference in blood vessel density between intramyocardial transplantation group and intracoronary transplantation group, but it was significantly higher than that in corresponding control group (P < 0.01). These finding suggest that intramyocardial and intracoronary transplantation of autologous bone marrow mononuclear cells can promote angiogenesis and collateral circulation formation in ischemic myocardium in the swines. Related Articles | Metrics

Select

Effects of CD34+ human umbilical cord blood cell transplantation in different time on cardiac function and vascular endothelial cell growth factor secrection in rats with myocardial infarction Xing Yun-li, Liu Jun-hua, Li Min, Wang Cui-ying 2012, 16 (23):  4267-4272.  doi: 10.3969/j.issn.1673-8225.2012.23.018 Abstract ( 336 )   PDF (681KB) ( 443 )   Save BACKGROUND: Stem cells transplantation can improve the cardiac function in the patients with myocardial infarction (MI) and improve the prognosis. OBJECTIVE: To study the effects of intravenous transplantation of CD34+ human umbilical cord blood cells in the different times on cardiac functions and cytokine secretion in rats with MI. METHODS: MI model was established by ligation of the left anterior descending coronary artery in rats. The experimental group and control group were injected with 0.5 mL CD34+ human umbilical cord blood cells and phosphate buffered saline through caudal vein at 1, 5, 10 and 30 days after MI, respectively. RESULTS AND CONCLUSION: Compared with control group, the ejection fraction of left ventricular was significantly increased at 5 and 10 days after infraction (P < 0.05), the left ventricular end-diastolic dimension was significantly induced (P < 0.05), the left ventricular posterior wall thickening and the capillary density was improved significantly (P < 0.05) and the number of vascular endothelial cell growth factor in the experimental group was the most (P < 0.05), especially at 10 days after infraction, the cardiac functions were improved most obviously (P < 0.05). Intravenous transplantation of CD34+ cells could improve the cardiac function at 5 days and 10 days after MI. At 10 days after infraction, the secretion of VEGF was increased, the angiogenesis was increased, and the cardiac function improvement was most significant. Cord blood mononuclear cell transplantation may improve the cardiac function through the increase of VEGF secretion and capillary density. Related Articles | Metrics

Select

In vitro proliferation of human bone marrow stromal cells after transfection by denovirus vector co-expressing human bone morphogenetic protein-2 and human fibroblast growth factor 2 genes Guo Wei-tao, Wang Hui, Liu Si-jing, Zeng Rong, Xiao Qi-xian, Chen Zi-qiu, Huang Yun, Wang Bin, Hu Zi-bing 2012, 16 (23):  4273-4278.  doi: 10.3969/j.issn.1673-8225.2012.23.019 Abstract ( 199 )   PDF (605KB) ( 316 )   Save BACKGROUND: Transfection of exogenous gene into tissue-engineered seed cells via recombinant adenovirus vector is the key to gene therapy of bone defects. OBJECTIVE: To investigate the effect of gene transfection on the proliferation of human bone marrow stromal stem cells that tranfected with adenovirus vectors co-expressing human bone morphogenetic protein-2 (hBMP-2) and human fibroblast growth factor 2 (hFGF-2). METHODS: The Ad-hBMP2-IRES-hFGF2 plasmids were transfected into human bone marrow stromal stem cells. The efficiency of transfection was evaluated by fluorescence microscope. Reverse transcriptase polymerase chain reaction was used to observe the successful transcription of hBMP-2 and hFGF-2 cNDA in bone marrow mesenchymal stem cells. Western blot assay was used to identify the protein expression of hBMP-2 and hFGF-2 genes. The cellular viability was determined by trypan blue staining and the changes of the cell proliferation were observed by flow cytometry assay. RESULTS AND CONCLUSION: The expression of hBMP-2 and hFGF-2 genes was confirmed at mRNA and protein levels. The cell viability was not changed after transfection. The flow cytometry assay showed that the percentage of proliferating cells was increased significantly in cell cycle. The Ad-hBMP2-IRES-hFGF2 plasmids could transfect human bone marrow stromal stem cells with high transfection efficiency and promote cell proliferation. Related Articles | Metrics

Select

In vitro migration of monocyte chemoattractant protein 1 and neural stem cells Dong Yue, Kang Bo, Ding Peng, Dai Xiao-sen, Wang Chong-qian, Wang Wei-min, Shang Ya-jun, Wang Jin-kun 2012, 16 (23):  4279-4283.  doi: 10.3969/j.issn.1673-8225.2012.23.020 Abstract ( 278 )   PDF (447KB) ( 402 )   Save BACKGROUND: Studies have shown that the chemokine stromal cell derived factor-1 and vascular endothelial growth factor have the ability to make neural stem cells (NSCs) migration. OBJECTIVE: To explore the effect of monocyte chemoattractant protein 1 (MCP-1) and its receptoron the migrati on of NSCs in vitro. METHODS: Hippocampal NSCs from SD fetal rats were isolated and cultured; meanwhile they could be amplified and identified in vitro; The expression of CCR2 was detected with immunofluorescence and RT-PCR; Effect of MCP-1 with different concentrations (50, 100, 200, 300, 500 ng/mL) on NSCs in vitro migration was obsened under agarose. RESULTS AND CONCLUSION: Immunofluorescence and RT-PCR results showed that NSCs from fetal rat hippocampal could express chemokine receptor CCR2; and the migration experiment under agarose showed that the MCP-1 with different concentrations could induce the migration of NSCs in vitro and with the higher the dose the better the migration. But the anti-CCR42 polyclonal antibodies could inhibit the migration of NSCs. Related Articles | Metrics

Select

Construction of eukaryotic expression vector of rat epidermal growth factor and ciliary neurotrophic factor and their expression in cos-7 cells He Jian, Yin Zong-sheng, Gao Wei-lu, Luo Qing-li, Zhang Sheng-quan 2012, 16 (23):  4284-4289.  doi: 10.3969/j.issn.1673-8225.2012.23.021 Abstract ( 221 )   PDF (469KB) ( 369 )   Save BACKGROUND: After activation, astrocytes exhibit the characteristics of neural stem cells and overexpress the receptor of epidermal growth factor (EGF), ciliary neurotrophic factor (CNTF), which improves complex internal environment and therefore benefits for neuronal differentiation of neural stem cells. OBJECTIVE: To construct the eukaryotic expression vectors of pSecTag2/Hygro B-EGF and pSecTag2/Hygro B-CNTF, and detect EGF and CNTF expression in cos-7 cells so as to provide experimental evidence for gene therapy on spinal cord injury. METHODS: The cDNA fragments of EGF and CNTF genes were amplified from total RNAs respectively. The amplified fragments were respectively inserted into eukaryotic expression vector pSecTag2/Hygro B to construct the recombined plasmid that encoded EGF and CNTF cDNA. The plasmids carrying EGF and CNTF genes were transfected alone respectively or cotransfected into cos-7 cells by liposome method. Then the protein expressions were detected by western blot method. RESULTS AND CONCLUSION: RT-PCR results confirmed that EGF and CNTF cDNAs were successfully cloned. DNA sequence analysis confirmed that EGF and CNTF cDNAs in the constructed vectors were consistent with target sequences in the GenBank. Then two recombinant plasmids were cotransfected into cos-7 cells by liposome reagent. At 48 hours after transfection, EGF and CNTF protein expressions in cos-7 cells with the molecular weight of Mr6 000, 22 000 were identified by western blot analysis. These findings suggest that the eukaryotic expression vectors of pSecTag2/Hygro B-EGF and pSecTag2/Hygro B-CNTF were successfully constructed and they co-express EGF and CNTF after transfected into cos-7 cells. Related Articles | Metrics

Select

Effect evaluation of autologous bone marrow mesenchymal stem cell transplantation on motor function in children with cerebral palsy Liu Yan-song, Wang Xiao-dong, Yang Jing, Hua Rong-rong, Cheng Hong-bin, Liu Ya-fei, An Yi-hua 2012, 16 (23):  4290-4295.  doi: 10.3969/j.issn.1673-8225.2012.23.022 Abstract ( 246 )   PDF (499KB) ( 386 )   Save BACKGROUND: Cerebral palsy is described as a group of permanent disorders affecting motor development and posture, resulting in activity limitation. Although there are many kinds of functional therapy programs for cerebral palsy, their effects are limited. Stem cell based therapy, a new prospective therapy for central nervous system disorders, has the potential to repair and replace damaged brain tissue, and alleviate neurological deficits and motor function in cerebral palsy. OBJECTIVE: Using Gross Motor Function Measure-88 (GMFM88) and Fine Motor Function Measure-45 (FMFM45) scale to evaluate the effect of autologous bone marrow mesenchymal stem cell transplantation on motor function in children with cerebral palsy. METHODS: Twenty cerebral palsy children received autologous bone marrow mesenchymal stem cell transplantation and their motor functions were measured using GMFM88 scale and FMFM45 at baseline (the day before treatment) and 1, 3 and 6 months after treatment. RESULTS AND CONCLUSION: Seventeen subjects successfully accomplished the clinical study. Results of statistical analysis by SPSS13.0 software showed that (1) The A, B, C functional area scores and total score of GMFM88 increased significantly at 1, 3 and 6 months after bone marrow mesenchymal stem cell transplantation than that at baseline (P < 0.05). (2) The B, C functional area scores and total score of FMFM45 increased significantly at 1, 3 and 6 months after bone marrow mesenchymal stem cell transplantation than that at baseline (P < 0.05). During the present study period, no events had happened. Bone marrow mesenchymal stem cell transplantation is a safe, effective and feasible method to treat cerebral palsy patients. The gross and fine motor function was most rapidly improved in 1 month after the therapy and it could be continuously improved within 6 months after the therapy. Related Articles | Metrics

Select

Effects of stem cells transplantation on cardiac electrical activity Guo Yi-hong, Zhang Xiao-gang 2012, 16 (23):  4296-4300.  doi: 10.3969/j.issn.1673-8225.2012.23.023 Abstract ( 173 )   PDF (577KB) ( 288 )   Save BACKGROUND: Myocardial ion channel is one of the most important electrophysiological characteristics. But the changes in ion channels during the process of stem cells differentiation into cardiac cells and the impact on the electrophysiological properties of myocardial tissue remain unclear. OBJECTIVE: To summarize the effects of stem cells transplantation on cardiac electrical activity. METHODS: VIP database, PubMed database and SpringerLink database (2000-01/2011-05) were used to search the related articles about the effect of stem cells transplantation on cardiac electrical activity. The key words were “bone marrow mesenchymal stem cells, embryonic stem cells, induced pluripotent stem cells, cardiac electrical activity, ion channels” in English and in Chinese. RESULTS AND CONCLUSION: A total of 21 articles were included to review. The life threatening arrhythmia caused by stem cells transplantation has not been observed in a larger amount of clinical experiments at home or abroad, but some cardiac electrical activities and ion channel dynamics were changed after stem cells transplantation, the effects of stem cells transplantation on cardiac electrical activity should not be underestimated. The induced pluripotent stem cells can not only differentiate into myocardial cells, but also have the similar electrical activities with them. Therefore, it must be wildly studied and applied in the regenerative treatment of damaged myocardium. Related Articles | Metrics

Select

Research and prospects of stem cells transplantation for the treatment of Alzheimer’s disease Yu Cheng-cheng, Zhang Bin, Chen Hu 2012, 16 (23):  4301-4305.  doi: 10.3969/j.issn.1673-8225.2012.23.024 Abstract ( 263 )   PDF (522KB) ( 440 )   Save BACKGROUND: Neural stem cells (NSCs) and bone marrow-derived mesenchsymal stem cells transplantation would alleviate the symptoms of Alzheimer’s disease patients and therefore shows the potential to treat Alzheimer’s disease. OBJECTIVE: To review the research development of stem cells therapy for Alzheimer's disease, and to discusses its potential applications. METHODS: The articles about stem cells transplantation for the treatment of Alzheimer's disease were retrieved in CNKI database and PubMed database from January 1981 to August 2011. The index words in titles and abstracts were “Alzheimer’s disease, stem cells, mesenchymal stem cells, transplantation”. The articles related to stem cells transplantation for the treatment of Alzheimer's disease and those which were recently published in famous Scholarly Journals in the same field were selected. At last, we got two hundred and forty-one theses. According to the inclusion criteria, 28 documents were finally selected. RESULTS AND CONCLUSION: Understanding of the pathological changes and pathogenesis of Alzheimer's disease would be helpful to select sensitive biological markers and carry out effective preventions and treatments for Alzheimer's disease. Mitochondrial DNA changes of oxygen stress and the immune responses in Alzheimer's disease played a key role in the pathological process of Alzheimer's disease, and maybe have a good synergistic effect. The mechanism research regarding stem cells transplantation for treatment of Alzheimer's disease can guide the clinical applications. Related Articles | Metrics

Select

Research progress of allogeneic bone marrow mesenchymal stem cells Wang Hong-bin 2012, 16 (23):  4306-4309.  doi: 10.3969/j.issn.1673-8225.2012.23.025 Abstract ( 300 )   PDF (454KB) ( 358 )   Save BACKGROUND: Autologous bone marrow mesenchymal stem cells transplantation is a new method in recent years for the treatment of articular cartilage defects. However, in the clinic, the autologous bone marrow mesenchymal stem cells cannot be used to repair the acute osteochondral defect in a short time, and allogeneic bone marrow mesenchymal stem cells can be in advance prepared for the application. OBJECTIVE: To symmetrically analyze the immunomodulation activity and inhibition studies of allogeneic bone marrow mesenchymal stem cells. METHODS: The PubMed database and Wanfang database from 1990 to 2009 were used to search the articles describing to the immunomodulation activity and inhibition studies of allogeneic bone marrow mesenchymal stem cells by the first author with the key words of “bone mesenchymal stem cells, immunomodulation activity, immunosuppression” in English and Chinese. RESULTS AND CONCLUSION: Although the transplantation of allogeneic cells may be attacked by the immune system, bone marrow mesenchymal stem cell is an exception, as it is the regulatory T cell that can suppress the immune response. Bone marrow mesenchymal stem cells can inhibit the T cells, B cells, natural killer cells and antigen presenting cells. Bone marrow mesenchymal stem cells are the tissue engineering cell group that isolated from the bone marrow; it has the immunosuppression related marks, and can inhibit the proliferation of allogeneic T cells in vitro, so the bone marrow mesenchymal stem cells can be used for allogeneic cell therapy. Bone marrow mesenchymal stem cells provide a new method for the repair of articular cartilage defects in the clinic by allogeneic bone marrow mesenchymal stem cells. Related Articles | Metrics

Select

Development of adipose-derived stem cells and their transplantation for treatment of cerebral ischemia Jiang Wei, Liang Guo-biao, Liu Min-pei 2012, 16 (23):  4310-4315.  doi: 10.3969/j.issn.1673-8225.2012.23.026 Abstract ( 224 )   PDF (573KB) ( 285 )   Save BACKGROUND: Studies show that transplantation of adipose-derived stem cells (ADSCs) can improve the neurological functions of animals in treatment of cerebral ischemia. OBJECTIVE: To summarize the literatures on the biological characteristics, differentiation, and applications in ischemic diseases of ADSCs. METHODS: A computer-based online search of literatures published from January 2005 to December 2010 related to the development of adipose-derived stem cells and their transplantation for treatment of cerebral ischemia was performed in CNKI database and PubMed database using the key words of “adipose-derived stem cells, therapy of transplant, cerebral ischemia”. The documents associated with the development of adipose-derived stem cells and their transplantation for treatment of cerebral ischemia were selected, and those published in 3-5 years or in authoritative journals were preferred in the same field. Totally 152 articles in English and Chinese were obtained in initial retrieval, and 38 of them were chosen to summarize based on inclusion criteria. RESULTS AND CONCLUSION: ADSCs have many advantages including easy-to-obtain, rich resources, rapid expansion, non-tumorigenesis, low immunogenicity, and non-ethical constraints. Preclinical experiments have demonstrated that transplantation of ADSCs can improve the neurological functions, reduce the region of small cerebral infarction, promote angiogenesis, and express neuron-specific markers. The improvement of neurological functions was proved in experiments with different ways and times of ADSCs transplantation, but the mechanism is still unclear. The treatment of ischemic disease by ADSCs transplantation needs more research to consummate the mechanism. Related Articles | Metrics

Select

Stem cells and lung transplantation Mao Wen-jun, Chen Jing-yu 2012, 16 (23):  4316-4322.  doi: 10.3969/j.issn.1673-8225.2012.23.027 Abstract ( 285 )   PDF (627KB) ( 365 )   Save BACKGROUND: Lung transplantation is the only therapeutic option for patients with various end-stage lung diseases; however, it has high risk for death during the perioperative period. Stem cells have a good application prospect in the basic and clinical research of lung repair after lung transplantation. OBJECTIVE: To summarize the biological characteristics of stem cells and the interaction of immune system, and to introduce the application status of stem cells in lung transplantation experiment and clinical research. METHODS: We searched the PubMed database, Springlink database, and Science Direct database from January 1990 to March 2011 with the key words of “lung, pulmonary, transplantation, stem cell” in English. An online search of CNKI database, Wanfang database, Chongqing VIP database from January 1990 to March 2011 was also conducted with the key words of “lung, pulmonary, transplantation, stem cell” in Chinese. A total of 156 literatures were screened out, and 50 documents were included in the review based on the inclusion and exclusion criteria. RESULTS AND CONCLUSION: Stem cells played an important role in tissue repair by replacing the damaged lung tissue. It could reduce cell apoptosis, inflammatory reaction and pulmonary fibrosis through paracrine effect. Mesenchymal stem cells could induce immune tolerance of organ transplantation due to its low immunogenicity, which reflected in two aspects, on one hand, mesenchymal stem cells could reduce the immunological rejection by regulating several cytokines, and on the other hand, it could reduce the immune response by inhibiting the activity of T-lymphocytes, B-lymphocytes, natural killer cells, dendritic cells, and neutrophilic granulocytes. Based on the unique biological characteristics, stem cells played an important role in the induction of immune gomphosis and reducing the chronic rejection after lung transplant, which facilitate cell and gene therapy mediated by stem cells. Related Articles | Metrics

Select

Progress of autologous hematopoietic stem cells transplantation in the treatment of lymphoma Guo Yi-gang, Feng Xue-lian, Liu Lin 2012, 16 (23):  4323-4328.  doi: 10.3969/j.issn.1673-8225.2012.23.028 Abstract ( 193 )   PDF (628KB) ( 641 )   Save BACKGROUND: Autologous hematopoietic stem cells transplantation is an effective scheme for treatment of lymphoma. OBJECTIVE: To summarize the progress of autologous hematopoietic stem cells transplantation in the treatment of lymphoma METHODS: We retrieved PubMed database, CNKI database, Wanfang database, Vip database, digital journals of Chinese Medical Association and Google network database for articles published from January 1990 to February 2011, which was about autologous hematopoietic stem cells transplantation in the treatment of lymphoma. The key words were “autologous hematopoietic stem cells transplantation, lymphoma”. A total of 371 articles were retrieved and 31 articles were included according to the inclusion criteria. RESULTS AND CONCLUSION: Hundreds of thousands patients with lymphoma have been cured since autologous hematopoietic stem cells transplantation was developed in 1980s. Before transplantation, the pretreatment scheme from simple high-dose chemotherapy regimen at first evolved into combined with rituximab and isotope-labeled monoclonal antibody; the rituximab developed from a purification medication before pretreatment to an important medication that was used before and after autologous hematopoietic stem cells transplantation for a long time. However, autologous hematopoietic stem cells transplantation, as a backup of hematopoietic system for patients promotes hemopoietic system recovery quickly after pretreatment. With further development, autologous hematopoietic stem cells transplantation will become the important scheme of lymphoma treatments. Related Articles | Metrics

Select

Biological effects of hypoxia on mesenchymal stem cells Wang Li-wei, Huang Wen, Zhao Yu 2012, 16 (23):  4329-4334.  doi: 10.3969/j.issn.1673-8225.2012.23.029 Abstract ( 216 )   PDF (561KB) ( 699 )   Save BACKGROUND: Oxygen is involved in the synthesis of ATP in cells for cellular metabolism, and the concentration of oxygen is also an important factor to control the physiological functions of cells. Physiological and pathological events of bone marrow mesenchymal stem cells always occur under hypoxia condition, but the mechanism is still unclear. OBJECTIVE: To overview the research progress of the biological effects of hypoxia on mesenchymal stem cells. METHODS: A computer retrieval of PubMed database, Wanfang database, Weipu database and CNKI database (1997-01/2011-10) was conducted to search the related articles with the keywords of “mesenchymal stem cells, hypoxia, hypoxia-induced factor” in English and Chinese. A total of 1 120 articles were retrieved, and finally 48 articles were included in final analysis according to the inclusion criteria. RESULTS AND CONCLUSION: Hypoxia induction could reduce the apoptosis of bone marrow mesenchymal stem cells by upregulating the expression of vascular endothelial growth factor and anti-apoptotic protein Bcl-2 and downregulating the expression of p53 apoptotic signaling. Hypoxia could increase the proliferation of bone marrow mesenchymal stem cells by inhibiting WNT signaling pathway and bone morphogenetic protein signaling pathway. Hypoxic tissue could induce the migration of mesenchymal stem cells to hypoxic region, the main signal pathways in this process include vascular endothelial growth factor signaling pathway, stromal cell-derived factor-1 signaling pathway and c-Met signaling pathway. Hypoxia could also increase the adhesion of mesenchymal stem cells mainly through the high expression of cytoskeletal proteins and cell-cell adhesion molecule. Hypoxia could promote cell differentiation by increasing the expression of angiopoietin-1 and vascular endothelial growth factor and other factors. Related Articles | Metrics

Select

Induced differentiation of umbilical cord blood mesenchymal stem cells into neuron-like cells Chen Lei, Chen Bing, Xie Fu-tang 2012, 16 (23):  4335-4338.  doi: 10.3969/j.issn.1673-8225.2012.23.030 Abstract ( 215 )   PDF (575KB) ( 420 )   Save BACKGROUND: Umbilical cord blood-derived mesenchymal stem cells have similar multipotential differentiation to hematopoietic stem cells and bone marrow mesenchymal stem cells, and can be induced to differentiate into neuron-like cells. However, the success rate of isolation and culture is very low. So it is necessary to search a method for culture of umbilical cord blood-derived mesenchymal stem cells with higher success rate. OBJECTIVE: To explore the biological characteristics of umbilical cord blood-derived mesenchymal stem cells and to search a method for induced differentiation into neuron-like cells. METHODS: A computer-based online retrieval of CNKI database was performed to search papers describing induced differentiation of umbilical cord blood-derived mesenchymal stem cells into neuron-like cells published between 2002-2011 using the key words “umbilical cord blood-derived mesenchymal stem cells”, “neural cells”, “induced differentiation” and “cytokines”. RESULTS AND CONCLUSION: Umbilical cord blood-derived stem cells show similar multipotential differentiation to hematopoietic stem cells and bone marrow mesenchymal stem cells and can be induce-differentiated into neuron-like cells. They are negative for CD34 and therefore are the main marker for differentiating hematopoietic stem cells and bone marrow mesenchymal stem cells. The methods for in vitro induced differentiation of umbilical cord blood-derived mesenchymal stem cells include cell growth factor method, chemical inducers method, growth factors combined with inducers method. Research from China and abroad shows that neuron-like cells induce-differentiated by umbilical cord blood-derived mesenchymal stem cells can be used for treatment of central nervous system diseases, and umbilical cord blood-derived mesenchymal stem cells are a kind of ideal seed cells for repair of nerve injury. Related Articles | Metrics

Select

Transplantation of hematopoietic stem cells for treatment of thalassemia Wu Xue-dong, Jing Yuan-fang, Wen Jian-yun, Liu Hua-ying, Wang Yan-hua, Li Chun-fu 2012, 16 (23):  4339-4348.  doi: 10.3969/j.issn.1673-8225.2012.23.031 Abstract ( 452 )   PDF (701KB) ( 575 )   Save BACKGROUND: Hematopoietic stem cell transplantation is the best way to cure thalassemia, including bone marrow transplant, umbilical cord blood transplantation, intrauterine hematopoietic stem cell transplantation, peripheral blood stem cell transplantation. OBJECTIVE: To multivariately analyze the literature on the hematopoietic stem cell transplantation for thalassemia through Science Citation Index database and its analysis tool. METHODS: A computer-based retrieval was performed for the literature of hematopoietic stem cell transplantation for treatment of thalassemia with the key words hematopoietic stem cell, transplantation, thalassaemia or thalassemia, beta thalassaemia or beta thalassemia or β thalassaemia or β thalassemia published between January 2002 and December 2011 in SCI database. The retrieval results were analyzed, and the trends were described in words and graphics. Articles on the hematopoietic stem cell transplantation for thalassemia including the following types: (1) peer-reviewed original papers; (2) meeting abstracts; (3) proceedings papers; (4) reviews; (5) letters; (6) editorial materials; (7) corrections; (8) book chapter; (9) news items. Exclusive criteria included (1) articles unrelated to hematopoietic stem cell transplantation for thalassemia; (2) articles published before 2002; (3) articles which were not published on journals. RESULTS AND CONCLUSION: A total of 8 981 literatures on the hematopoietic stem cell transplantation for thalassemia were retrieved in SCI database, most of which were published as original articles. Seven articles were identified as classic literature. The overall number of literature had an upward trend from 2002 to 2011. Blood published most papers in this field (n=1515, 16.87%). This paper provides a valuable reference for researchers to understand the overview and present situation of this field in order to set further research. Related Articles | Metrics

Select

Research progress of stem cell therapy for type 1 diabetes mellitus Hu Mei-jun, Pan Xing-hua, Pang Rong-qing, Cai Xue-min 2012, 16 (23):  4349-4353.  doi: 10.3969/j.issn.1673-8225.2012.23.033032 Abstract ( 297 )   PDF (512KB) ( 635 )   Save BACKGROUND: The possible effective way for the treatment of type 1 diabetes mellitus is to rebuild the secreting system of insulin through pancreatic or pancreatic islet transplantation. However, limited donors and rejection after transplantation often lead to failed treatment. OBJECTIVE: To summarize the study progress in embryonic stem cells, somatic stem cells and induced pluripotent stem cells for the treatment of type 1 diabetes mellitus. METHODS: Articles addressing stem cells for the treatment of type 1 diabetes mellitus were retrieved online in CNKI database and PubMed database from January 2008 to December 2011 with the key words of “embryonic stem cells, somatic stem cells, induced pluripotent stem cells, type 1 diabetes mellitus” in Chinese and English in the title and abstract. The articles related to stem cells for the treatment of type 1 diabetes were included. For the articles in the same field, those published recently or in the authorize journals were preferred. A total of 128 literatures were obtained, and 33 documents of them were involved for review according to the inclusion criteria. RESULTS AND CONCLUSION: Recently, different sources of stem cells could be induced into insulin-producing cells in vivo or in vitro, and the induced cells were used in the treatment of animal model with type 1 diabetes. It showed certaub therapeutic efficacy and even made recipient’s blood sugar level returned to normal. But controversy exists regarding whether stem cells took part in islet cell regeneration or repair. Related Articles | Metrics

Select

Application of bone marrow mesenchymal stem cells transplantation for the treatment of spinal cord injury Wang Ge, Wu Dong-cheng 2012, 16 (23):  4354-4358.  doi: 10.3969/j.issn.1673-8225.2012.23.033 Abstract ( 304 )   PDF (592KB) ( 436 )   Save BACKGROUND: At present, there lacks effective repair approach for atrophy and necrosis of spinal nerve of spinal cord injury patients in the clinic. Bone marrow mesenchymal stem cells transplantation for the treatment of spinal cord injury has wide application prospects. OBJECTIVE: To summarize the progress of bone marrow mesenchymal stem cells transplantation for the treatment of spinal cord injury. METHODS: The first author searched PubMed database (http://www.ncbi.nlm.nih.gov/PubMed) and Wanfang database (http://g.wanfangdata.com.cn/) for literatures concerning bone marrow mesenchymal stem cells, spinal cord injury and cell transplantation from 2001 to 2011. Key words were “bone marrow mesenchymal cells, spinal cord injury, cell transplantation” in English. Duplicate articles were excluded. Finally, 32 articles were selected to summarize. RESULTS AND CONCLUSION: The research of bone marrow mesenchymal stem cells transplantation for treatment of spinal cord injury has made achievements in the spinal cord injury model establishment, transplantation time and approach, concentration and combined transplantation. Although clinical trials have been carried out at home and abroad, the mechanism was not very clear yet. The safety and efficacy of clinical treatment need further investigation. Related Articles | Metrics

Select

Hematopoietic stem cell transplantation for treating hematological malignancies Wu Bi-jia, Gong Jun-mei, Guo Tian-jian, Chen Wan-hong, Li Na 2012, 16 (23):  4359-4366.  doi: 10.3969/j.issn.1673-8225.2012.23.034 Abstract ( 319 )   PDF (531KB) ( 377 )   Save BACKGROUND: In recent years, with the continuous development of hematology and related fields of basic and clinical research, hematopoietic stem cell transplantation has become an effective means of treatment for hematologic malignancies, or even the only way to cure certain blood diseases. OBJECTIVE: According to the literature retrieval results, to comparatively analyze the literatures on the hematopoietic stem cell transplantation for treating hematological malignancies in SCI database and to explore the future development tendency of hematopoietic stem cell transplantation for treating hematological malignancies. METHODS: A retrieval was performed for the literature of the hematopoietic stem cell transplantation for treating hematological malignancies, using key words of “hematopoietic stem cell, transplantation, acute myelogenous leukemia, non-hodgkin lymphoma, chronic myelocytic leukemia” between 2001-01 and 2010-12 in SCI database. The retrieval results were analyzed, and the trends were described in words and graphics. RESULTS AND CONCLUSION: The overall literatures of the hematopoietic stem cell transplantation for treating hematological malignancies had an upward trend from 2001 to 2010. United States published more literature than other countries, which play an important role in the field of the hematopoietic stem cell transplantation for treating hematological malignancies. Most of literatures were published from Fred Hutchinson Cancer Research Center. This paper provides a valuable reference for researchers to understand the overview and present situation of this field. Related Articles | Metrics

Select

Changes in cellular growth factor levels in cerebrospinal fluid of autism patients before and after human umbilical cord blood mononuclear cell transplantation Li Qiang, Lü Yong-tao, Huan Ying, Liu Min, Ge Ru-cun, Chen Xing-wang, Qi Hong-Shun, Gao Bao-Feng, Hu Xiang, An Lei 2012, 16 (23):  4367-4370.  doi: 10.3969/j.issn.1673-8225.2012.23.035 Abstract ( 260 )   PDF (381KB) ( 372 )   Save BACKGROUND: Studies have demonstrated that umbilical cord blood mononuclear cells exhibit some effects in treatment of autism. OBJECTIVE: To detect levels of nerve growth factor (NGF), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in cerebrospinal fluid of autism patients treated with umbilical cord blood mononuclear cells. METHODS: A total of 30 autism children received treatment by injection of human umbilical cord blood mononuclear cells via lumbar puncture subarachnoid cavity, once a week, for three times totally. RESULTS AND CONCLUSION: The levels of nerve growth factors were significantly greater at 1 week after the second treatment than pretreatment and 1 week after the first treatment (P < 0.05). The levels of vascular endothelial growth factors and basic fibroblast growth factors were slightly, but not significantly, higher at 1 week after the second treatment than pretreatment and at 1 week after the first treatment (P > 0.05). Results suggest that the levels of nerve growth factors in cerebrospinal fluid were significantly increased in autism children who received treatment of umbilical cord blood mononuclear cells, however, the levels of vascular endothelial growth factors and basic fibroblast growth factors were not influenced significantly. Related Articles | Metrics