Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (23): 4209-4212.doi: 10.3969/j.issn.1673-8225.2012.23.006

Previous Articles     Next Articles

Effect of intermittent treatment with parathyroid hormone on human bone marrow stromal stem cells

Yin Xiao-xue, Zhou Fang, Zhang Zhi-shan, Song Chun-li   

  1. Department of Orthopedics, Third Hospital of Peking University, Beijing 100083, China
  • Received:2011-10-26 Revised:2011-12-01 Online:2012-06-03 Published:2013-11-06
  • Contact: Zhou Fang, M.D., Chief physician, Professor, Department of Orthopedics, Third Hospital of Peking University, Beijing 100083, China
  • About author:Yin Xiao-xue☆, M.D., Associate researcher, Department of Orthopedics, Third Hospital of Peking University, Beijing 100083, China luckyemail2008@sina.com

PDF

364

Abstract:

BACKGROUND: Continuous and intermittent application of parathyroid hormone at different doses can cause different effects on bone.
OBJECTIVE: To observe the effect of intermittent treatment with different doses of parathyroid hormone on the proliferation and differentiation of human bone marrow stromal stem cells.
METHODS: Human bone marrow stromal stem cells were cultured in vitro and divided into blank control group and 5, 20 and 50 nmo/L parathyroid hormone group, and the parathyroid hormone groups were treated for 1 hour per day. MTT and alkaline phosphatase activities were used to detect the proliferation and differentiation of human bone marrow stromal stem cells to osteoblasts.
RESULTS AND CONCLUSION: 50 nmol/L of parathyroid hormone treated for 1 hour per day could greatly promote the proliferation of human bone marrow stromal stem cells and could increase the alkaline phosphatase activities, and the alkaline phosphatase activities in 50 nmo/L parathyroid hormone group were higher than other three groups. Intermittent treatment with 50 nmol/L parathyroid hormone can promote the proliferation and differentiation of human bone marrow stromal stem cells.

CLC Number: