Expression of aldehyde dehydrogenase 3B1 during human bone marrow mesenchymal stem cells senescence

doi:10.12307/2022.007

Abstract

Abstract: BACKGROUND: Human bone marrow mesenchymal stem cells have the phenomenon of cell aging during passage in vitro, but the mechanism by which it occurs is not yet clear. Aldehyde dehydrogenase 3B1 has antioxidant effect. Oxidative stress is one of the important factors leading to cell senescence. Whether the expression level of aldehyde dehydrogenase 3B1 is related to human bone marrow mesenchymal stem cells senescence is still unclear.
OBJECTIVE: To observe the expression of aldehyde dehydrogenase 3B1 in cellular senescence, providing a new research direction for aging mechanism of stem cells.
METHODS: The natural senescent model of human bone marrow mesenchymal stem cells was established and identified. The mRNA expression of aldehyde dehydrogenase 3B1 in human bone marrow mesenchymal stem cells was detected by bisulfite methylation sequencing and real-time qPCR. The protein level of aldehyde dehydrogenase 3B1 was detected by immunofluorescence and western blot assay.
RESULTS AND CONCLUSION: (1) Human bone marrow mesenchymal stem cells were cultured in subculture. β-Galactosidase staining showed that positive cells increased continuously, and the positive cells were the most obvious at passage 7 to 13. (2) Methylation sequencing showed that in passage 16 human bone marrow mesenchymal stem cells, the methylation level of aldehyde dehydrogenase 3B1 was down regulated. (3) The mRNA and protein levels of aldehyde dehydrogenase 3B1 were down regulated during the passage of human bone marrow mesenchymal stem cells in vitro. (4) The results show that aldehyde dehydrogenase 3B1 is down regulated in the natural senescence of human bone marrow mesenchymal stem cells, but its mechanism needs further study.

Key words: stem cells, bone marrow mesenchymal stem cells, cellular senescence, natural senescence, aldehyde dehydrogenase 3B1, model

CLC Number:

Mai Liping, He Guodong, Chen Shaoxian, Zhu Jiening, Hou Xinghua, Zhang Mengzhen, Li Xiaohong. Expression of aldehyde dehydrogenase 3B1 during human bone marrow mesenchymal stem cells senescence[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(1): 40-44.

Figures/Tables 5

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