Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (7): 1005-1011.doi: 10.12307/2022.136
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Hou Jingying, Guo Tianzhu, Yu Menglei, Long Huibao, Wu Hao
Received:
2020-06-05
Revised:
2020-06-06
Accepted:
2020-06-20
Online:
2022-03-08
Published:
2021-10-29
Contact:
Hou Jingying, MD, Associate chief physician, Emergency Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China
About author:
Hou Jingying, MD, Associate chief physician, Emergency Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China
Supported by:
CLC Number:
Hou Jingying, Guo Tianzhu, Yu Menglei, Long Huibao, Wu Hao. Hypoxia preconditioning targets and downregulates miR-195 and promotes bone marrow mesenchymal stem cell survival and pro-angiogenic potential by activating MALAT1 [J]. Chinese Journal of Tissue Engineering Research, 2022, 26(7): 1005-1011.
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2.1 骨髓间充质干细胞增殖情况 各组0 h的吸光度值差异无显著性意义(P > 0.05),而缺氧组24 h的吸光度值明显高于常氧组(0.400±0.002 vs. 0.300±0.000,P < 0.01),缺氧+ si-MALAT1组的吸光度值明显低于缺氧组和缺氧+si-MALAT1对照组(P < 0.01),3组分别为0.290±0.004,0.400±0.002和0.410±0.004;缺氧+miR-195组的吸光度值明显低于缺氧组和缺氧+miR-195对照组(P < 0.01),3组分别为0.310±0.006,0.400±0.002和0.410±0.004,见图1A。细胞增殖率比较结果显示:缺氧组细胞增殖率也较常氧组显著升高,两者分别为(233.33±8.70)%和(130.80±5.20)% (P < 0.01);缺氧+si-MALAT1组的增殖率明显低于缺氧组和缺氧组+si-MALAT1对照组,3组分别为(123.08±4.50)%,(233.33±8.70)%和(215.38±7.40)% (P < 0.01),缺氧+miR-195组的增殖率明显低于缺氧组和缺氧+miR-195对照组,3组分别为(158.33±5.30)%,(233.33±8.70)%和(215.38±6.80)% (P < 0.01),见图1B。"
2.4 MALAT1和miR-195表达 采用qRT-PCR检测各组MALAT1和 miR-195的表达,结果显示:与常氧组相比,缺氧组MALAT1表达升高,miR-195表达下降,差异有显著性意义(P < 0.01)。缺氧+si-MALAT1组的MALAT1表达明显低于缺氧组和缺氧+si-MALAT1对照组(P < 0.01),而miR-195的表达则明显高于缺氧组和缺氧+si-MALAT1对照组(P < 0.01)。缺氧+miR-195组MALAT1的表达明显低于缺氧组和缺氧+miR-195对照组(P < 0.01),而miR-195的表达则明显高于缺氧组和缺氧+miR-195对照组(P < 0.01),见图4A和4B。"
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