Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (7): 1074-1079.doi: 10.12307/2022.147
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Wang Xinmin, Liu Fei, Xu Jie, Bai Yuxi, Lü Jian
Received:
2021-03-16
Revised:
2021-03-17
Accepted:
2021-06-01
Online:
2022-03-08
Published:
2021-10-29
Contact:
Liu Fei, Professor, Master’s supervisor, Ward Two, Department of Orthopedics, First Hospital of Qinhuangdao, Qinhuangdao 066000, Hebei Province, China
About author:
Wang Xinmin, MD, Associate chief physician, Ward Two, Department of Orthopedics, First Hospital of Qinhuangdao, Qinhuangdao 066000, Hebei Province, China
Supported by:
CLC Number:
Wang Xinmin, Liu Fei, Xu Jie, Bai Yuxi, Lü Jian. Core decompression combined with dental pulp stem cells in the treatment of steroid-associated femoral head necrosis in rabbits[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(7): 1074-1079.
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2.1 一般情况 模型组注射醋酸泼尼松龙三四周时能明显观察到右侧股骨变细,摄食量减小,反应迟钝,体态偏瘦;髓芯减压术后1周内,溶媒组和干细胞组实验动物活动及摄食量减少,动物精神萎靡,术后2周活动量及摄食量增加,3周时活动增多,摄食量及活动逐渐恢复正常。所有52只实验动物生存状态良好,无死亡,模型组有5只、溶媒组有3只、干细胞组有1只实验动物轻度跛行。 2.2 X射线片观察结果 各组标本在钻孔缺损区以外变化大致相似,其中正常对照组股骨头外形正常,椭圆形,密度无明显变化,骨小梁结构正常,见图1;模型组股骨头骨小梁走形及结构出现不规则紊乱,密度较正常对照组明显增高,软骨塌陷明显,出现软骨下囊性病变;模型组、溶媒组股骨头软骨比牙髓干细胞组塌陷更明显,溶媒组、牙髓干细胞组钻孔缺损内有着不同的变化,牙髓干细胞组钻孔缺损区可见类似骨小梁结构,钻孔缺损区中心密度低于溶媒组,两组钻孔区外边缘密度有所增高,未见明显差别,见图1。"
2.4 Micro-CT检测结果 牙髓干细胞组隧道内及出口处出现均匀的高密度信号,骨隧道周围出现不规则的骨小梁,尤其在骨隧道远端,软骨下骨区域;溶媒组、牙髓干细胞组隧道内均可见新生骨质长入,隧道与周围界限不清,呈逐渐融合状态,其中牙髓干细胞组更明显;模型组股骨头内骨小梁稀松排列,明显不规则,骨小梁轻度不均并斑点状稀疏区,4组股骨头外形大致正常,均未见到明显塌陷,见图3。采用Micro-CT对兔股骨头进行扫描并三维重建,使用CT-AN软件进行三维分析,并进行感兴趣区域骨密度测定,结果显示:溶媒组和牙髓干细胞组均较模型组有显著改善,差异有显著性意义(P < 0.05);与溶媒组比较,牙髓干细胞组股骨头骨体积分数、骨小梁数量、骨密度改善更明显,差异有显著性意义(P < 0.05),见表1。"
2.5.2 Masson染色结果 模型组可见大多数骨组织呈绿色,骨小梁混乱且不规则,骨小梁中骨细胞空骨陷窝明显多于溶媒组和牙髓干细胞组;溶媒组骨组织中呈现红绿相间分布,红染区域较模型组明显增多,隧道内出现片状或条状被红染的成熟骨组织;牙髓干细胞组红色成熟骨组织多于模型组和溶媒组,其中红染区域比例高于模型组和溶媒组,骨髓内脂肪细胞较模型组和溶媒组减少,但绿染区域较正常对照组明显增多。 2.6 空骨陷窝率及骨小梁面积比 牙髓干细胞组空骨陷窝率和骨小梁面积比与正常对照组更为接近,两组间比较差异无显著性意义,溶媒组空骨陷窝率高于正常对照组,骨小梁面积比低于正常对照组,两组间比较差异有显著性意义,见图6和图7;溶媒组、牙髓干细胞组空骨陷窝率明显低于模型组,骨小梁面积比明显高于模型组;与溶媒组相比,牙髓干细胞组空骨陷窝率及骨小梁面积比更接近于正常对照组,差异有显著性意义,见表2。 2.7 生物相容性 治疗12周后溶媒组、牙髓干细胞组髓芯减压隧道内组织相容性好,并未发现明显的细胞排斥反应及炎性细胞浸润。 "
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