Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (23): 4237-4241.doi: 10.3969/j.issn.1673-8225.2012.23.012

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Effects of stromal cell-derived factor-1 and its receptor CXCR4 on the migration of endogenous neural stem cells

Dai Xiao-sen, Ding Peng, Dong Yue, Mu Lin-jie, Wang Wei-min, Wang Chong-qian, Wang Jin-kun   

  1. Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China
  • Received:2011-10-06 Revised:2011-11-30 Online:2012-06-03 Published:2013-11-06
  • Contact: Wang Jin-kun, Chief physician, Master’s supervisor, Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China
  • About author:Dai Xiao-sen★, Studying for master’s degree, Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China Daixiaosen123456@qq.com

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Abstract:

BACKGROUND: Neural stem cells own the characteristics of migration in vitro. However, the mechanisms underlying the migration of these cells remain unclear.
OBJECTIVE: To investigate the effects of stromal cell-derived factor-1 and its receptor CXCR4 on the migration of neural stem cells in vivo.
METHODS: The brain injured rat model was established with cortical micro-injection of lipopolysaccharide. At 3, 5, and 7 days after modeling, the brain tissue was harvested. Immunohistochemical staining of frozen section was used to detect the expression of stromal cell-derived factor-1 and nestin-positive cells in different times and phages.
RESULTS AND CONCLUSION: Immunohistochemical staining showed that the expression of stromal cell-derived factor-1 was in a time-dependent manner. Nestin-positive cells extended and migrated outward significantly and pointed to the lipopolysaccharide injection area. Stromal cell-derived factor-1 can induce the endogenous neural stem cells to move towards lipopolysaccharide injection area in vivo.

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