Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (23): 4213-4216.doi: 10.3969/j.issn.1673-8225.2012.23.007

Previous Articles     Next Articles

Co-culture of umbilical cord blood-derived monocytes and bone marrow mesenchymal stem cells

Wang Lei1, Song Jie2, Kuang Tao2   

  1. 1Department of Hematology, Qingdao Municipal Hospital, Qingdao 266071, Shandong Province, China;
    2Department of Special Health Care, Affiliated Hospital of Medical College of Qingdao University, Qingdao 266003, Shandong Province, China
  • Received:2011-11-23 Revised:2012-03-15 Online:2012-06-03 Published:2013-11-06
  • Contact: Kuang Tao, Associate chief physician, Department of Special Health Care, Affiliated Hospital of Medical College of Qingdao University, Qingdao 266003, Shandong Province, China kuangtk@126.com
  • About author:Wang Lei, Associate chief physician, Department of Hematology, Qingdao Municipal Hospital, Qingdao 266071, Shandong Province, China qingdaowanglei108@sina.com

PDF

306

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells support hematopoiesis in vivo and in vitro and play an important role in regulation of hematopoiesis.
OBJECTIVE: To investigate the biological characteristics and multi-directional differentiation capability of bone marrow mesenchymal stem cells and their ability to support hematopoiesis in vivo and in vitro.
METHODS: Bone marrow monocytes were isolated by density gradient culture method. Bone marrow mesenchymal stem cell phenotype was detected by flow cytometry. Umbilical cord monocytes were inoculated onto bone marrow mesenchymal stem cell trophoblast for co-culture. Granulocyte-monocyte colony forming units were counted.
RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells showed typical fibroblast-like cell morphology and strongly expressed CD44 and CD29, but they were negative for CD34 and CD106. Bone marrow mesenchymal stem cells could promote the proliferation of umbilical cord monocytes to form hematopoietic progenitor colonies. These findings suggest that bone marrow mesenchymal stem cells support hematopoiesis.

CLC Number: