Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (23): 4232-4236.doi: 10.3969/j.issn.1673-8225.2012.23.011

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Differentiation of adipose-derived stem cells into islet β-like cells in vitro

Yang Guang1, Zhao Zhan-zhi2, Zhu Xin-bing1, Hu Song1   

  1. 1Department of General Surgery;
    2Department of Anesthesiology, the Central Hospital of Liaohe Oil Field, Panjin 124000, Liaoning Province, China
  • Received:2011-11-03 Revised:2011-12-10 Online:2012-06-03 Published:2013-11-06
  • About author:Yang Guang★, Master, Associate chief physician, Department of General Surgery, the Central Hospital of Liaohe Oil Field, Panjin 124000, Liaoning Province, China guangyang1967@qq.com

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Abstract:

BACKGROUND: Islet β-like cells can be induce-differentiated by embryonic stem cells, pancreatic stem cells, hepatic oval cells, intestinal stem cells and bone marrow mesenchymal stem cells in vivo. Then whether adipose-derived stem cells can be differentiate into islet β-like cells remains pooly understod?
OBJECTIVE: To investigate the feasibility of adipose-derived stem cells to differentiate into islet β-like cells.
METHODS: Adipose-derived stem cells were isolated and cultured from rabbit liposuction, and after two generations passage, the adipose-derived stem cells were differentiated into islet β-like cells induced by high glucose, basic fibroblast growth factorand nicotamine in culture medium.
RESULTS AND CONCLUSION: After the passage 2 adipose-derived stem cells were induced by high glucose, the adipose-derived stem cells formed cell clusters and dithizone-stained cell clusters in flask were observed. Immunocytochemistry also confirmed that these cell clusters were positive for insulin. Adipose-derived stem cells can differentiate into islet β-cells in vitro. These islet β-cells can synthesize and store insulin.

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