Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (23): 4279-4283.doi: 10.3969/j.issn.1673-8225.2012.23.020

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In vitro migration of monocyte chemoattractant protein 1 and neural stem cells

Dong Yue1, Kang Bo2, Ding Peng1, Dai Xiao-sen1, Wang Chong-qian1, Wang Wei-min1, Shang Ya-jun1, Wang Jin-kun1   

  1. 1Department of Neurosurgery, First Affiliated Hospital of Kunming Medical College, Kunming 650000, Yunnan Province, China;
    2Department of Neurosurgery, Yan’an Hospital of Kunming, Kunming 650000, Yunnan Province, China
  • Received:2011-09-29 Revised:2011-11-14 Online:2012-06-03 Published:2013-11-06
  • Contact: Wang Jin-kun, Professor, Master’s supervisor, Department of Neurosurgery, First Affiliated Hospital of Kunming Medical College, Kunming 650000, Yunnan Province, China
  • About author:Dong Yue★, Studying for master’s degree, Department of Neurosurgery, First Affiliated Hospital of Kunming Medical College, Kunming 650000, Yunnan Province, China Dongyue2005110@126.com

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Abstract:

BACKGROUND: Studies have shown that the chemokine stromal cell derived factor-1 and vascular endothelial growth factor have the ability to make neural stem cells (NSCs) migration.
OBJECTIVE: To explore the effect of monocyte chemoattractant protein 1 (MCP-1) and its receptoron the migrati on of NSCs in vitro.
METHODS: Hippocampal NSCs from SD fetal rats were isolated and cultured; meanwhile they could be amplified and identified in vitro; The expression of CCR2 was detected with immunofluorescence and RT-PCR; Effect of MCP-1 with different concentrations (50, 100, 200, 300, 500 ng/mL) on NSCs in vitro migration was obsened under agarose.
RESULTS AND CONCLUSION: Immunofluorescence and RT-PCR results showed that NSCs from fetal rat hippocampal could express chemokine receptor CCR2; and the migration experiment under agarose showed that the MCP-1 with different concentrations could induce the migration of NSCs in vitro and with the higher the dose the better the migration. But the anti-CCR42 polyclonal antibodies could inhibit the migration of NSCs.

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