Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (23): 4199-4203.doi: 10.3969/j.issn.1673-8225.2012.23.004

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Effects of platelet-rich plasma gel on the proliferation and osteogenic activity of rabbit bone marrow mesenchymal stem cells

Zhang Lin-pu1, 2, Dai Xiao-hua2, Wang Guan-hua2, Li Yan-ni2, Lian Xiao-li2, Yao Hui2   

  1. 1Stomatology College of Tianjin Medical University, Tianjin 300070, China;
    2Tianjin Stomatological Hospital of Nankai University, Tianjin 300041, China
  • Received:2011-10-03 Revised:2011-10-20 Online:2012-06-03 Published:2013-11-06
  • Contact: Yao Hui, Chief physician, Tianjin Stomatological Hospital of Nankai University, Tianjin 300041, China, Tianjin 300041, China yaoh2k@163.com
  • About author:Zhang Lin-pu, Associate chief physician, Stomatology College of Tianjin Medical University, Tianjin 300070, China; Tianjin Stomatological Hospital of Nankai University, Tianjin 300041, China tjkqzlp2006@sina.com

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Abstract:

BACKGROUND: Platelet-rich plasma (PRP) contains several kinds of growth factors related to bone repair, and can form fibrin meshwork scaffold after agglomerated by activation, which is beneficial to cells adhesion and promotes bone regeneration.
OBJECTIVE: To evaluate the proliferation and osteogenic activity of rabbit bone marrow mesenchymal stem cells (rBMSCs) in platelet-rich plasma gel in vitro.
METHODS: rBMSCs were isolated from bone marrow of limb long bone of 5-day neonatal rabbits by adhesion culture. PRP was obtained from adult rabbit peripheral venous blood. Experiment samples were divided into three groups: PRP plus rBMSCs (PRP/rBMSCs) group, PRP plus α-MEM medium (PRP/α-MEM) group and rBMSCs group.
RESULTS AND CONCLUSION: The difference of lactate dehydrogenase activity among three groups was significant (P < 0.05), and the cell proliferation in PRP/rBMSCs group was obviously better than that in the other two groups. The PRP/rBMSCs gel was complete, uniform and semitransparent. It had many cells which were anomalistic polygon with long synapse distributing in gel. Tetracycline labeled flavo-green fluorescence brightened dots and nodes were discovered in PRP/rBMSCs gel after 2 and 3 weeks of culture using fluorescence microscope. Calcify nodes were formed in the PRP/rBMSCs gel. The number and size of fluorescence brightened dots and nodes founded in the third week after culture were a little more and bigger than those found in the second week. There were no fluorescence brightened dots and nodes in PRP/α-MEM gel. It indicates that rBMSCs combined with PRP gel can promote the proliferation and osteogenic activity of rBMSCs.

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