Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (23): 4193-4198.doi: 10.3969/j.issn.1673-8225.2012.23.003

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Effect of matrix metalloproteinase-1 on myogenic differentiation of mouse bone marrow-derived mesenchymal stem cells in vitro

Zheng Zhen-yang1, Zhang Wei-xi1, Leng Yan2, Zhou Chen1, Ma Zhen-yu1, Zhong Zhi-gang1   

  1. 1Department of Neurology, 2Department of Rehabilitation, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
  • Received:2011-11-08 Revised:2012-01-07 Online:2012-06-03 Published:2013-11-06
  • Contact: Zhang Wei-xi, Doctor, Professor, Chief physician, Doctor’s supervisor, Department of Neurology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China weixizhang@qq.com.
  • About author:Zheng Zhen-yang★, Studying for master’s degree, Department of Neurology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China xiaotang_1986@yahoo.com.cn.

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Abstract:

BACKGROUND: The myogenic differentiation potential of bone marrow-derived mesenchymal stem cells (MSCs) in vitro from mice is low.
OBJECTIVE: To explore the effect of matrix metalloproteinase-1 (MMP-1) on myogenic differentiation of MSCs in vitro from mice.
METHODS: After MSCs were isolated and cultured by differential adhesion, as well as identified by cell surface markers and multi-lineage differentiation potential, the cells were assigned to 4 groups according to various MMP-1 concentration gradients (10 μg/L group, 1 μg/L group, 0.1 μg/L group and control group). The mRNA expressions of MyoD and Desmin were detected by real-time quantitative PCR, and the expression of protein Desmin was tested by Western Blotting.
RESULTS AND CONCLUSION: MSCs obtained from differential adhesion were of morphological homogenicity, and they were CD29+, Sca-1+, CD45- and CD34-. Additionally, MSCs were capable of multi-lineage differentiation. The mRNA expression of Desmin and MyoD as well as the expression of protein Desmin were up regulated via MMP-1 inducement. Moreover, the up-regulation degree of mRNA and protein expression was concentration-dependent. These suggest that MMP-1 can promote MSCs to differentiate into muscle cells in vitro.

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