Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (23): 4187-4192.doi: 10.3969/j.issn.1673-8225.2012.23.002

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Bone marrow-derived mesenchymal stem cell transplantation increased expression of neural cell adhesion molecules in a rat model of ischemic brain injury

Peng Yu, Zhang Qi-mei, You Hui, Zhang Ying, Zhuang Wei-hua, Zhang Qiang, Zhou Jing-hua   

  1. First Clinical College of China Three Gorges University & Yichang Central People’s Hospital, Yichang 443003, Hubei Province, China
  • Received:2012-01-01 Revised:2012-03-28 Online:2012-06-03 Published:2013-11-06
  • Contact: Zhang Qi-mei, Professor, Chief physician, First Clinical College of China Three Gorges University & Yichang Central People’s Hospital, Yichang 443003, Hubei Province, China
  • About author:Peng Yu, Master, Associate chief physician, First Clinical College of China Three Gorges University & Yichang Central People’s Hospital, Yichang 443003, Hubei Province, China ycpengyu@126.com You Hui, Associate chief physician, First Clinical College of China Three Gorges University & Yichang Central People’s Hospital, Yichang 443003, Hubei Province, China

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Abstract:

BACKGROUND: Studies have demonstrated that transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) improves neurofunctional recovery and the underlying mechanism may be related to upregulated expression of neural cell adhesion molecules.
OBJECTIVE: To investigate the effects of BMSC transplantation on the expression of neural cell adhesion molecule after ischemic brain injury in rats.
METHODS: Sprague-Dawley rats were randomly divided into a sham-operated group and a middle cerebral artery occlusion (MCAO) group. A permanent focal cerebral ischemia model was established using modified Longa’s method. The MCAO groups were randomly subdivided into a BMSC group (transplantation of 5×105 allogenic BMSCs via the left lateral ventricle) and a control group (phosphate buffered saline injection). The sham-operated group received neither MCAO nor BMSC transplantation.
RESULTS AND CONCLUSION: At 7 and 14 days following transplantation, neural cell adhesion molecule expression surrounding the infarct foci was significantly higher in the BMSC group and control group (P < 0.01), and the expression in the BMSC group was significantly higher than in the control group (P < 0.05). At 14 days following transplantation, neurological severity score in the BMSC group was significantly lower than in the control group (P < 0.05). BMSC transplantation promoted neurofunctional recovery by upregulating neural cell adhesion molecule expression in the marginal zone of cerebral infarct focus of a rat model of focal cerebral ischemia.

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