Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (23): 4181-4186.doi: 10.3969/j.issn.1673-8225.2012.23.001

    Next Articles

Expression of annexin A1 in rabbit bone marrow mesenchymal stem cells during their early differentiation into osteoblasts and adipocytes in vitro

Pan Xin-yuan1, Liang Bo-wei2, Zhao Jin-min2, Yin Guo-qian1   

  1. 1Department of Plastic and Aesthetic Surgery,
    2Department of Trauma and Hand Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China;
  • Received:2011-10-18 Revised:2011-11-02 Online:2012-06-03 Published:2013-11-06
  • Contact: Zhao Jin-min, Doctoral supervisor, Professor, Chief physician, Department of Trauma and Hand Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China zhaojinmin@126.com
  • About author:Pan Xin-yuan★, Studying for master’s degree, Department of Plastic and Aesthetic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China Liang Bo-wei★, Master, Attending physician, Department of Trauma and Hand Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China LBW5515@163.com u2coldplay@163.com

PDF

329

Abstract:

BACKGROUND: Studies have reported different views on the expression of annexin A1 (ANX A1) in bone marrow mesenchymal stem cells (BMSCs) during their differentiation.
OBJECTIVE: To investigate the expression of ANX A1 in rabbit BMSCs during their differentiation into osteoblasts and adipocytes in vitro.
METHODS: BMSCs were isolated by whole bone marrow adherence method, and then the cells were cultured in media with osteogenic inducer, adipogenic inducer and non-inducer respectively.
RESULTS AND CONCLUSION: The expression of ANX A1 mRNA was obviously decreased during the cell differentiation into osteoblasts compared with those without differentiation (P < 0.01). In contrast, the expression of ANX A1 mRNA was obviously increased in BMSCs cultured in medium with adipogenic inducer (P < 0.01). Osteogenic inducer could inhibit the growth of BMSCs to achicve an increase in apoptosis (P < 0.01), while the adipogenic inducer had little effects on cell growth and apoptosis (P > 0.05). ANX A1 may play an important role in regulation of BMSCs differentiation into adipocytes in vitro without the role of inducer. However, the association with osteogenic differentiation is uncertain.

CLC Number: