Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (23): 4267-4272.doi: 10.3969/j.issn.1673-8225.2012.23.018

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Effects of CD34+ human umbilical cord blood cell transplantation in different time on cardiac function and vascular endothelial cell growth factor secrection in rats with myocardial infarction

Xing Yun-li1, Liu Jun-hua2, Li Min1, Wang Cui-ying1   

  1. 1Medical Health Center, Affiliated Beijing Friendship Hospital of Capital Medical University, Beijing 100050, China;
    2Dazhalan Community Healthy Center, Beijing 100050, China
  • Received:2011-10-09 Revised:2011-12-01 Online:2012-06-03 Published:2013-11-06
  • About author:Xing Yun-li☆, M.D., Attending physician, Medical Health Center, Affiliated Beijing Friendship Hospital of Capital Medical University, Beijing 100050, China xingyunli@medmail.com.cn

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Abstract:

BACKGROUND: Stem cells transplantation can improve the cardiac function in the patients with myocardial infarction (MI) and improve the prognosis.
OBJECTIVE: To study the effects of intravenous transplantation of CD34+ human umbilical cord blood cells in the different times on cardiac functions and cytokine secretion in rats with MI.
METHODS: MI model was established by ligation of the left anterior descending coronary artery in rats. The experimental group and control group were injected with 0.5 mL CD34+ human umbilical cord blood cells and phosphate buffered saline through caudal vein at 1, 5, 10 and 30 days after MI, respectively.
RESULTS AND CONCLUSION: Compared with control group, the ejection fraction of left ventricular was significantly increased at 5 and 10 days after infraction (P < 0.05), the left ventricular end-diastolic dimension was significantly induced (P < 0.05), the left ventricular posterior wall thickening and the capillary density was improved significantly (P < 0.05) and the number of vascular endothelial cell growth factor in the experimental group was the most (P < 0.05), especially at 10 days after infraction, the cardiac functions were improved most obviously (P < 0.05). Intravenous transplantation of CD34+ cells could improve the cardiac function at 5 days and 10 days after MI. At 10 days after infraction, the secretion of VEGF was increased, the angiogenesis was increased, and the cardiac function improvement was most significant. Cord blood mononuclear cell transplantation may improve the cardiac function through the increase of VEGF secretion and capillary density.

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