Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (23): 4263-4266.doi: 10.3969/j.issn.1673-8225.2012.23.017

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Angiogenesis of autologous bone marrow mononuclear cells by intramyocardial and intracoronary transplantation into ischemic myocardium of a swine model of acute myocardial infarction

Sun Lin, Chu Tian-shu, Bai Wen-wei, Wang Li-qin, Zhang Ge, Ruan Zhi-min   

  1. Department of Cardiovascular Medicine, Second Affiliated Hospital of Kunming Medical College, Room for Coronary Heart Disease Research, Yunnan Institute of Cardiovascular Disease, Kunming 650101, Yunnan Province, China
  • Received:2011-11-17 Revised:2012-02-11 Online:2012-06-03 Published:2013-11-06
  • Contact: Ruan Zhi-min, Associate professor, Department of Cardiovascular Medicine, Second Affiliated Hospital of Kunming Medical College, Room for Coronary Heart Disease Research, Yunnan Institute of Cardiovascular Disease, Kunming 650101, Yunnan Province, China ruan_zhimin@126.com
  • About author:Sun Lin☆, M.D., Associate professor, Department of Cardiovascular Medicine, Second Affiliated Hospital of Kunming Medical College, Room for Coronary Heart Disease Research, Yunnan Institute of Cardiovascular Disease, Kunming 650101, Yunnan Province, China sunlinkm@sina.com

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Abstract:

BACKGROUND: Current evidence exists that intramyocardial transplantation of bone marrow mononuclear cells can promote angiogenesis and blood supply in ischemic myocardium.
OBJECTIVE: To investigate the effects of intramyocardial and intracoronary transplantation of autologous bone marrow mononuclear cells into ischemic myocardium of a swine model of acute myocardial infarction.
METHODS: Twenty-two swine models of acute myocardial infarction were randomly divided into four groups. In the intramyocardial transplantation group, immediately after induction of acute myocardial infarction, autologous bone marrow mononuclear cell suspension was injected into the ischemic myocardium. In the intramyocardial transplantation control group, autologous bone marrow mononuclear cell suspension was replaced by Hank’s balanced salt solution. In the intracoronary transplantation group, at 1 week after induction of acute myocardial infarction, autologous bone marrow mononuclear cell suspension was injected into the left coronary artery. In the intracoronary transplantation control group, autologous bone marrow mononuclear cell suspension was replaced by Hank’s balanced salt solution.
RESULTS AND CONCLUSION: At 1 week after transplantation, there were no significant differences in serum levels of basic fibroblast growth factor and vascular endothelial growth factor between intramyocardial transplantation group and intracoronary transplantation group. The serum levels of basic fibroblast growth factor and vascular endothelial growth factor in intramyocardial transplantation group or intracoronary transplantation group were significantly higher than those in corresponding control group (P < 0.01). At 4 weeks after transplantation, there was no significant difference in blood vessel density between intramyocardial transplantation group and intracoronary transplantation group, but it was significantly higher than that in corresponding control group (P < 0.01). These finding suggest that intramyocardial and intracoronary transplantation of autologous bone marrow mononuclear cells can promote angiogenesis and collateral circulation formation in ischemic myocardium in the swines.

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