Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (23): 4253-4258.doi: 10.3969/j.issn.1673-8225.2012.23.015

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Transforming growth factor beta 1 promotes the migration of mesenchymal stem cells via upregulation of Snail expression

Zheng Li-fang1, 2, Mei Yuan-wu2, Zhang Xiao-qiao2, Zhang Hong2   

  1. 1Department of Neurology, Tianyou Hospital, Wuhan University of Science and Technology, Wuhan 430064, Hubei Province, China;
    2Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
  • Received:2011-11-19 Revised:2012-02-03 Online:2012-06-03 Published:2013-11-06
  • Contact: Mei Yuan-wu, Chief physician, Doctoral supervisor, Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China meiyuanwu@sohu.com
  • About author:Zheng Li-fang☆, Doctor, Associate chief physician, Department of Neurology, Tianyou Hospital, Wuhan University of Science and Technology, Wuhan 430064, Hubei Province, China; Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China zlf027@163.com

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Abstract:

BACKGROUND: Transformation growth factor β1 (TGF-β1) can promote bone marrow mesenchymal stem cells (BMSCs) migration and proliferation, but the underlying mechanisms remain unclear.
OBJECTIVE: To observe the invasiveness of TGF-β1 on BMSCs cultured in vitro, and to investigate regulatory effect on Snail and matrix metalloproteinase 2 (MMP-2) expression.
METHODS: Rat BMSCs were isolated and cultured with density gradient centrifugalization and adherence method. The influence of different concentrations of TGF-β1 on the BMSC migration was detected using the modified Transwell chambers. Small interfering RNA for Snail gene was synthesized and transfected into BMSCs by liposomel before TGF-β1 was treated, and the expression of Snail and MMP-2 before and after transfection were measured by western blot assay.
RESULTS AND CONCLUSION: The exogenous TGF-β1 can induce a dose-dependent increase in cell migration, which peaked at 2 μg/L. The expression levels of Snail mRNA and MMP-2 mRNA were significantly increased after 2 μg/L TGF-β1 treatment. Snail gene can effectively inhibit the expression of MMP-2 promoted by TGF-β1. Experimental findings indicate that TGF-β1 could increase the MMP-2 expression and then promote the BMSCs migration through the upregulation of the Snail expression.

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