中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (10): 1896-1900.doi: 10.3969/j.issn.2095-4344.2013.10.029

• 干细胞学术探讨 stem cell academic discussion • 上一篇    

骨髓间充质干细胞成骨分化中的微小RNA及Wnt通路

邹翰林,郭永飞,刘 岩   

  1. 解放军第二军医大学附属长征医院骨科,上海市 200003
  • 收稿日期:2012-06-05 修回日期:2012-07-23 出版日期:2013-03-05 发布日期:2013-03-05
  • 通讯作者: 刘岩,硕士,主任医师,解放军第二军医大学附属长征医院骨科,上海市 200003
  • 作者简介:邹翰林★,男,1985年生,山东省威海市人,汉族,2012年解放军第二军医大学毕业,硕士,主要从事创伤骨科方面的研究。zouhanlin2009@163.com
  • 基金资助:

    国家自然科学基金项目(31100988)。

Role of microRNA and Wnt signaling pathway in osteogenic differentiation of bone marrow mesenchymal stem cells

Zou Han-lin, Guo Yong-fei, Liu Yan   

  1. Department of Orthopedics, Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China
  • Received:2012-06-05 Revised:2012-07-23 Online:2013-03-05 Published:2013-03-05
  • Contact: Liu Yan, Master, Chief physician, Department of Orthopedics, Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China
  • About author:Zou Han-lin★, Master, Department of Orthopedics, Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China zouhanlin2009@163.com

摘要:

背景:有差异性表达的微小RNA在成骨细胞的增殖和分化中发挥广泛而又重要的作用,但其作用机制、作用靶点还不甚清楚,是近年来的研究热点。
目的:对微小RNA通过Wnt信号通路调节成骨分化方面的研究现状及新进展作一综述。
方法:应用计算机检索2000年1月至2012年1月CNKI和PubMed数据库,在标题和摘要中以“MicroRNA,Wnt通路,成骨分化”或“MicroRNA,Wnt pathway,osteogenesis”为检索词进行检索。选择文章内容与微小RNA经典Wnt通路促进成骨分化有关者,同一领域文献则选择近期发表或发表在权威杂志的文章。初检得到205篇文献,最终选择33篇文献进行综述。
结果与结论:微小RNA是近年来生命科学的研究热点,越来越多的研究表明微小RNA作用于经典Wnt信号通路在成骨分化过程中发挥了重要作用。了解微小RNA在成骨分化中的调控机制是骨组织工程化培养的基础,具有巨大的应用前景。目前关于微小RNA的研究工作还处于起步阶段。

关键词: 干细胞, 干细胞综述, 骨髓间充质干细胞, Wnt信号通路, MicroRNA, 成骨分化, 国家自然科学基金

Abstract:

BACKGROUND: Differentially expressed microRNA plays an important role in proliferation and differentiation of osteoblasts. However, the underlying mechanism and possible targets are poorly understood.
OBJECTIVE: To review the recent research progress of the role of microRNA in osteogenic differentiation of bone marrow mesenchymal stem cells by Wnt signaling pathway.
METHODS: A computer-based online retrieval of CNKI and Pubmed databases was performed to search papers describing the role of microRNA in osteogenesis published during January 2000 to January 2012 using key words “microRNA, Wnt signaling pathway, osteogenesis”. In the same research field, papers published recently or in high impact factor journals were selected. A total of 205 papers were initially retrieved, and 33 were suitable for final analysis.
RESULTS AND CONCLUSION: MicroRNA is increasingly searched in the field of life science. Accumulative evidence exists that microRNA plays an important role in osteogenic differentiation via Wnt signaling pathway. A better understanding of the mechanism by which RNA regulates osteogenic differentiation is the basis for culture of tissue-engineered bone and shows great application prospect. At present, the research of microRNA is still in its infancy.

Key words: stem cells, stem cell review, bone marrow mesenchymal stem cells, Wnt signaling pathway, MicroRNA, osteogenesis, National Natural Science Foundation of China

中图分类号: