中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (45): 7273-7278.doi: 10.3969/j.issn.2095-4344.2014.45.011

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

接受干细胞移植重型β地中海贫血患者部分细胞因子水平与移植物抗宿主病的关系

陈丽白,温建芸,阮永胜,裴夫瑜,刘华颖,何岳林,李春富,吴学东   

  1. 南方医科大学南方医院儿科,广东省广州市 510515
  • 出版日期:2014-11-05 发布日期:2014-11-05
  • 通讯作者: 吴学东,主任医师,南方医科大学南方医院儿科,广东省广州市 510515
  • 作者简介:陈丽白,1988年生,湖南省株洲市人,汉族,2012年吉首大学医学院毕业,医师,主要从事小儿血液病及造血干细胞移植研究。
  • 基金资助:

    广东省科技计划项目(2011B031800280)

Association between some cytokines and graft-versus-host disease after allogeneic hematopoietic stem cell transplantation for beta-thalassemia major

 Chen Li-bai, Wen Jian-yun, Ruan Yong-sheng, Pei Fu-yu, Liu Hua-ying, He Yue-lin, Li Chun-fu, Wu Xue-dong   

  1. Department of Pediatrics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • Online:2014-11-05 Published:2014-11-05
  • Contact: Wu Xue-dong, Chief physician, Department of Pediatrics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • About author:Chen Li-bai, Physician, Department of Pediatrics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • Supported by:

    the Scientific and Technological Plan of Guangdong Province, No. 2011B031800280

摘要:

背景:细胞因子在移植物抗宿主病的发生发展过程中起至关重要的作用,但目前缺乏其在重型β地中海贫血中的研究报道。
目的:探讨细胞因子与重型β地中海贫血患者造血干细胞移植后发生移植物抗宿主病之间的关系。
方法:采用液相芯片技术检测11例重型β地中海贫血移植物抗宿主病出现前,出现时,出现第4天,第7天,完全消失时白细胞介素6、白细胞介素8、白细胞介素12、肿瘤坏死因子α、巨噬细胞游走抑制因子的动态变化。
结果与结论:各时相白细胞介素6、白细胞介素12、肿瘤坏死因子α、巨噬细胞游走抑制因子水平差异有显著性意义,移植物抗宿主病出现时各细胞因子水平最高,移植物抗宿主病出现第7天次之。各时相白细胞介素8水平差异有显著性意义,移植物抗宿主病出现第4天最高。以上结果提示移植物抗宿主病发生前后白细胞介素6、白细胞介素8、白细胞介素12、肿瘤坏死因子α、巨噬细胞游走抑制因子动态水平变化在一定程度上反映移植后患者的免疫状态,可作为辅助诊断移植物抗宿主病的监测指标。


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


全文链接:

关键词: 干细胞, 移植, 重型β地中海贫血, 移造血干细胞移植, 植物抗宿主疾病, 白细胞介素6, 白细胞介素8, 白细胞介素12, 肿瘤坏死因子α, 巨噬细胞游走抑制因子

Abstract:

BACKGROUND: Cytokines play an important role in the occurrence and development of graft-versus-host disease, but there is a current lack of reports on the association between cytokines and graft-versus-host disease after allogeneic hematopoietic stem cell transplantation for treatment of β-thalassemia major.
OBJECTIVE: To investigate the association between cytokines and graft-versus-host disease after allogeneic hematopoietic stem cell transplantation for β-thalassemia major.
METHODS: We observed the dynamic variation of interleukin 6, interleukin 8, interleukin 12, tumor necrosis factor-α and macrophage migration inhibitory factor in 11 children with β-thalassemia major before onset of graft-versus-host disease, when graft-versus-host disease occurred, at days 4 and 7 after onset of graft-versus-host disease, and when graft-versus-host disease disappeared.
RESULTS AND CONCLUSION: There was a significant difference in serum levels of interleukin-6, interleukin-12, tumor necrosis factor-α, macrophage migration inhibitory factor in different time points, and the highest levels of different cytokines appeared when graft-versus-host disease occurred, followed by those at 7 days after 
graft-versus-host disease. There was a significant difference in serum levels of interleukin-8 in different time points, and the highest level appeared at 4 days after graft-versus-host disease. The dynamic expression of interleukin-6, interleukin-8, interleukin-12, tumor necrosis factor-α, macrophage migration inhibitory factor can estimate the immune function of β-thalassemia major patients who develops graft-versus-host disease after allogeneic hematopoietic stem cell transplantation, and can be used as the immunobiology indicators for the early diagnosis of graft-versus-host disease.


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


全文链接:

Key words: hematopoietic stem cell transplantation, beta-thalassemia, graft vs host , cytokines

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