中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (51): 9570-9574.doi: 10.3969/j.issn.1673-8225.2011.51.017

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

血糖对树鼩感染表皮葡萄球菌清除能力及植入性材料细菌黏附性的影响

徐玉善1，王  曦2，许云敏3，雷玉洁4，贺  猛4，黄云超4   

  1. 昆明医学院第一附属医院，1内分泌与代谢病科，3检验科，云南省昆明市  650032；2昆明医学院第二附属医院胸心血管外科，云南省昆明市  650101；4昆明医学院第三附属医院胸心血管外科，云南省昆明市 650118
  • 收稿日期:2011-05-24 修回日期:2011-06-26 出版日期:2011-12-17 发布日期:2011-12-17
  • 通讯作者: 黄云超，教授，昆明医学院第三附属医院胸心血管外科，云南省昆明市 650118
  • 作者简介:徐玉善☆，女，1972年生，云南省潞西市人，傣族，1996年北京大学医学部毕业，博士，副主任医师，主要从事内分泌与代谢病方面的研究。 并列第一作者：王曦★，男，1973年生，宁夏回族自治区吴忠市人，蒙古族，1996年北京大学医学部毕业，硕士，主治医师，主要从事胸心血管外科方面的研究。
  • 基金资助:

    国家自然科学基金资助项目(30872555)；云南省省科技强省计划资助项目(2007C009Z)。

Effect of glucose on eliminating activity and adhesion of Staphylococcus epidermidis in Tupaia belangeri

Xu Yu-shan1, Wang Xi2, Xu Yun-min3, Lei Yu-jie4, He Meng4, Huang Yun-chao4   

  1. 1Department of Endocrinology and Metabolism, 3Department of Laboratory, First Affiliated Hospital of Kunming Medical College, Kunming  650032, Yunnan Province, China;    2Department of Cardiovascular Thoracic Surgery, Second Affiliated Hospital of Kunming Medical College, Kunming  650101, Yunnan Province, China; 4Department of Cardiovascular Thoracic Surgery, Third Affiliated Hospital of Kunming Medical College, Kunming  650118, Yunnan Province, China
  • Received:2011-05-24 Revised:2011-06-26 Online:2011-12-17 Published:2011-12-17
  • Contact: Huang Yun-chao, Professor, Department of Cardiovascular Thoracic Surgery, Third Affiliated Hospital of Kunming Medical College, Kunming 650118, Yunnan Province, China huangych2001@ yahoo.com.cn
  • About author:Xu Yu-shan☆, Doctor, Associate chief physician, Department of Endocrinology and Metabolism, First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China xuyushan1@ yahoo.com.cn Wang Xi★, Master, Attending physician, Department of Cardiovascular Thoracic Surgery, Second Affiliated Hospital of Kunming Medical College, Kunming 650101, Yunnan Province, China wangxi1a2b@ sina.com Xu Yu-shan and Wang Xi contributed equally to this study.
  • Supported by:

    the National Natural Science Foundation of China, No. 30872555*; Yunnan Province Science and Technology Project, No. 2007C009Z*

PDF

387

被引次数

6

摘要：

背景：高浓度葡萄糖培养条件下，细菌在生物材料表面有较强的生物膜形成能力。
目的：观察血糖升高对表皮葡萄球菌在动物体内清除能力及植入性生物材料上细菌生物膜形成的影响。
方法：注射链脲佐菌素建立高血糖树鼩模型(血糖≥11.1 mmol/L)，采用生物膜形成阳性与阴性表皮葡萄球菌感染高血糖与正常对照树鼩，并同时在动物股静脉内植入PVC导管。
结果与结论：感染生物膜形成阳性的表皮葡萄球菌株后，血糖≥11.1 mmol/L树鼩血液、心脏、肝脏、肾脏、胰腺的细菌感染率及菌落计数较正常对照组高(P < 0.05)；扫描电镜观察血糖≥11.1 mmol/L组植入生物材料上有明显的生物膜形成。感染生物膜形成阴性表皮葡萄球菌株后无论血糖高低，均未观察到生物膜形成。表明血糖升高不仅使植入生物材料树鼩的细菌清除能力下降，同时可诱导细菌在植入生物材料表面形成明显的生物膜。

关键词: 表皮葡萄球菌, 生物材料, 生物膜, 血糖, 树鼩

Abstract:

BACKGROUND: High-concentration glucose can induce the formation of biofilms in Staphylococcus epidermidis.
OBJECTIVE: To investigate the effect of high-concentration glucose on eliminating ability and adhesion of Staphylococcus epidermidis in Tupaia belangeri.
METHODS: Tupaia belangeri models of hyperglycaemia (≥11.1 mmoL/L) were induced by intraperitoneal injection of Streptozocin. PVC catheters were inserted into the left femoral vein, and Staphylococcus epidermidis with or without formation of biofilms was inoculated into the catheters.
RESULTS AND CONCLUSION: Following transfection of Staphylococcus epidermidis with formation of biofilms, there were higher rates of bacterial infection as well as higher bacterium colony number in the serum, heart, liver, kidney and pancreas of Tupai belangeri in hyperglycemia group (≥11.1 mmoL/L) as compared with the control group (P < 0.05). Detected by scanning electronic microscope, biofilm formation was remarkable in hyperglycemia group (≥11.1 mmoL/L) (P < 0.05). However, there was no formation of biofilms in hyperglycemia or control groups following transfection of Staphylococcus epidermidis without formation of biofilms. Hyperglycemia can induce the decreasing ability of eliminating bacteria and the increasing formation of biofilms on the surface of biomaterials transfected with Staphylococcus epidermidis.

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