中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (41): 7685-7688.doi: 10.3969/j.issn.1673-8225.2011.41.021

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

脑缺血再灌注模型大鼠跑台运动后海马神经再生和血管内皮生长因子mRNA的变化

杨若愚1，娄淑杰2，陈秀恩3   

  1. 1上海市黄浦区体育科学研究站，上海市 200010
    2上海体育学院运动科学学院，上海市  200438
    3上海华东医院康复医学科，上海市200040
  • 收稿日期:2011-04-06 修回日期:2011-07-12 出版日期:2011-10-08 发布日期:2011-10-08
  • 作者简介:杨若愚★，男，1980年生，江苏省扬州市人，硕士，2006年上海体育学院毕业，助理研究员。 ryyang999@ hotmail.com

Effects of treadmill running on hippocampal neurogenesis and mRNA expression in vascular endothelial growth factor in a rat model of ischemia and reperfusion injury

Yang Ruo-yu1, Lou Shu-jie2, Chen Xiu-en3   

  1. 1Research Station of Sport Science of Shanghai Huangpu District, Shanghai 200010, China
    2School of Sport Science, Shanghai University of Sport, Shanghai  200438, China
    3Department of Rehabilitation Medicine, Shanghai Huadong Hospital, Shanghai  200040, China
  • Received:2011-04-06 Revised:2011-07-12 Online:2011-10-08 Published:2011-10-08
  • About author:Yang Ruo-yu★, Assistant Researcher, Research Station of Sport Science of Shanghai Huangpu District, Shanghai 200010, China ryyang999@ hotmail.com

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325

被引次数

7

摘要：

背景：研究表明跑台运动能促进健康大鼠海马的神经细胞再生。
目的：观察跑台运动对脑缺血再灌注模型大鼠海马神经再生和血管内皮生长因子mRNA表达的影响。
方法：用线栓法阻塞大脑中动脉以建立单侧脑缺血再灌注模型大鼠，将建模成功大鼠随机分为跑台运动组和安静对照组，另设假手术组。安静对照组和假手术组大鼠安静饲养，跑台运动组进行7 d跑台运动。跑台运动组和安静对照组大鼠在每天跑台运动前腹腔注射5-溴脱氧尿嘧啶核苷溶液。
结果与结论：免疫组织化学染色结果显示，跑台运动组大鼠双侧海马及齿状回5-溴脱氧尿嘧啶核苷阳性表达细胞数量显著多于安静对照组(P < 0.01)。实时荧光定量PCR检测结果显示，跑台运动组大鼠海马血管内皮生长因子mRNA表达水平显著高于安静对照组和假手术组(P < 0.05)。结果证实，跑台运动能够明显促进脑缺血再灌注大鼠海马神经细胞的再生并上调海马组织血管内皮生长因子的表达。

关键词: 跑台运动, 缺血再灌注, 海马, 神经细胞, 血管内皮生长因子, 组织构建

Abstract:

BACKGROUND: Several studies have demonstrated that treadmill running can promote hippocampal neurogenesis in healthy rats.
OBJECTIVE: To investigate the effects of treadmill running on hippocampal neurogenesis and mRNA expression in vascular endothelial growth factors (VEGF) in a rat model of ischemia and reperfusion injury.
METHODS: The method of reversible middle cerebral artery occlusion (MCAO) was used to obtain rat models of ischemia and reperfusion injury. MCAO rat models were randomly divided into exercise group and control group. A sham-operated group was used. Rats in the control and sham-operated groups were fed without exercise. The exercise group rats were subjected to 7-day treadmill running. The exercise and control group rats were intraperitoneally injected with 5-bromodeoxyuridine (BrdU) solution.
RESULTS AND CONCLUSION: Immunohistochemical staining results showed that BrdU-positive cells in the bilateral hippocampus and dentate gyrus were significantly more in the exercise group than in the control group (P < 0.01). Real time quantitative PCR results showed that mRNA expression in VEGF was significantly greater in the exercise group than in the control and sham-operated groups (P < 0.05). The results suggest that appropriate treadmill running can promote hippocampal neurogenesis and further enhance the mRNA expression in hippocampal VEGF in ischemia and reperfusion adult rats.

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