中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (41): 7689-7692.doi: 10.3969/j.issn.1673-8225.2011.41.022

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

血清体积分数对人细胞因子诱导杀伤细胞增殖的影响

任思坡，韩光宇，拾  莉，谭  昆，耿跃春，杨钦湘，王  健   

  1. 徐州市医学科学研究所，江苏省徐州市，221006
  • 收稿日期:2011-05-05 修回日期:2011-08-16 出版日期:2011-10-08 发布日期:2011-10-08
  • 通讯作者: 王健，副研究员，徐州市医学科学研究所，江苏省徐州市，221006 xzsyks@yahoo.com.cn
  • 作者简介:任思坡★，男，1977年生，山东省枣庄市人，汉族，硕士，主管技师，主要从事DC-CIK细胞和脐血间充质干细胞的基础和临床应用研究。 rensipo@163. com
  • 基金资助:

    课题受徐州市科技局社会发展项目(XZ2006268，XM09B0044)资助，项目名称：细胞治疗临床与研究公共实验平台，CIK细胞的冻存及复苏后再诱导研究。

Effects of serum concentration on the proliferation of human cytokine induced killer cells

Ren Si-po, Han Guang-yu, Shi Li, Tan Kun, Geng Yue-chun, Yang Qin-xiang, Wang Jian   

  1. Xuzhou Institute of Medical Sciences, Xuzhou  221006, Jiangsu Province, China
  • Received:2011-05-05 Revised:2011-08-16 Online:2011-10-08 Published:2011-10-08
  • Contact: Wang Jian, Associate researcher, Xuzhou Institute of Medical Sciences, Xuzhou 221006, Jiangsu Province, China xzsyks@yahoo.com.cn
  • About author:Ren Si-po★, Master, Technician in charge, Xuzhou Institute of Medical Sciences, Xuzhou 221006, Jiangsu Province, China rensipo@163.com
  • Supported by:

    Social Development Program of Bureau of Science and Technology of Xuzhou City, No.XZ2006268*; XM09B004*

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377

被引次数

1

摘要：

背景：细胞因子诱导的杀伤细胞的培养多采用体积分数10%的血清，但关于血清体积分数对其生长特性的影响少有报道。
目的：观察细胞因子诱导的杀伤细胞在不同血清体积分数培养液中的增殖情况。
方法：分别配制血清体积分数为10%，20%和30% +重组人白细胞介素2等细胞因子的完全培养液培养人细胞因子诱导的杀伤细胞，使用流式细胞仪分析血清体积分数对细胞周期的影响，通过MTT法检测细胞因子诱导的杀伤细胞在不同血清体积分数培养液中的增殖，并观察细胞因子诱导的杀伤细胞的生长状态。
结果与结论：细胞因子诱导的杀伤细胞在体积分数10%血清培养液中增殖速率和增殖指数均最低，在体积分数30%血清培养液中最高，3组细胞的不同时间点的增殖速率差异均有显著性意义(P < 0.05或P < 0.01)。结果证实，体外培养的细胞因子诱导的杀伤细胞的增殖具有血清体积分数依赖性，高血清体积分数培养液的促细胞因子诱导的杀伤细胞增殖效应明显。

关键词: 血清体积分数, 细胞因子诱导的杀伤细胞, 增殖, 细胞培养, 组织工程

Abstract:

BACKGROUND: Cytokine induced killer (CIK) cells are cultured mostly in 10% serum, but there have been few studies describing the effects of serum concentration on growth characteristics of CIK cells.
OBJECTIVE: To investigate the proliferation of CIK cells in different concentrations of serum.
METHODS: CIK cells were cultured with 10%, 20% and 30% fetal bovine serum supplemented with recombinant human interleukin (IL-2). The effects of serum concentration on cell cycle were analyzed using flow cytometry. The proliferation of CIK in different concentrations of serum was determined by MTT assay and the growth characteristics of CIK cells were observed.
RESULTS AND CONCLUSION: The proliferation rate and proliferation index of CIK cells were the lowest in 10% serum and highest in 30% serum. There was statistical significance in proliferation rate among three groups (P < 0.05 or P < 0.01). The proliferation of CIK cells cultured are serum concentration-dependent in vitro.

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