中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (41): 7681-7684.doi: 10.3969/j.issn.1673-8225.2011.41.020

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

蛇床子素干预人增生性瘢痕成纤维细胞增殖及转化生长因子β1的表达

侯晓华1，陈  虹2，曹  波2   

  1. 1武警医学院附属医院烧伤整形科，天津市 300162
    2武警医学院生药学教研室，天津市 300162
  • 收稿日期:2011-03-18 修回日期:2011-03-28 出版日期:2011-10-08 发布日期:2011-10-08
  • 作者简介:侯晓华★，女，1973年生，汉族，1997年天津医科大学毕业，硕士，主治医师，主要从事烧伤及瘢痕治疗工作。 houxiaohua73@sina.com

Effects of osthole on fibroblast proliferation and transforming growth factor beta 1 expression in hypertrophic scar tissue

Hou Xiao-hua1, Chen Hong2, Cao Bo2   

  1. 1Department of Burn and Plastic Surgery, Affiliated Hospital of Medical College of Chinese People's Armed Police Forces, Tianjin  300162, China
    2Department of Pharmacognosy, Medical College of Chinese People’s Armed Police Forces, Tianjin  300162, China
  • Received:2011-03-18 Revised:2011-03-28 Online:2011-10-08 Published:2011-10-08
  • About author:Hou Xiao-hua★, Master, Attending physician, Department of Burn and Plastic Surgery, Affiliated Hospital of Medical College of Chinese People’s Armed Police Forces, Tianjin 300162, China houxiaohua73@sina.com

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摘要：

背景：蛇床子素对体外培养人增生性瘢痕成纤维细胞增殖和细胞分泌的转化生长因子β1有抑制作用，但其具体作用机制尚待进一步研究。
目的：体外观察蛇床子素对人增生性瘢痕成纤维细胞增殖以及对细胞转化生长因子β1的影响。
方法：体外原代培养人增生性瘢痕成纤维细胞，以不同浓度的蛇床子素作用于成纤维细胞，观察细胞形态的变化，应用MTT法和生长曲线法检测蛇床子素对细胞增殖活性的影响。免疫组织化学检测细胞转化生长因子β1的表达。
结果与结论：蛇床子素能明显抑制人增生性瘢痕成纤维细胞的生长。MTT法检测的IC50为(15.2±2.0) μmol/L，可以明显下调细胞转化生长因子β1的表达(P < 0.05)。说明蛇床子素对人增生性瘢痕成纤维细胞有很强的生长抑制作用并可以下调转化生长因子β1的表达。

关键词: 蛇床子素, 增生性瘢痕, 成纤维细胞, 细胞增殖, 转化生长因子&beta, 1

Abstract:

BACKGROUND: Osthole exhibits inhibitory effects on human fibroblast proliferation and transforming growth factor beta 1 (TGF-β1) expression in hypertrophic scar tissue, but the precise mechanisms remains poorly understood.
OBJECTIVE: To investigate the effects of osthole on human fibroblast proliferation and TGF-β1 expression in hypertrophic scar tissue.
METHODS: Human hypertrophic scar fibroblasts cells were cultured in vitro and then treated by osthole at different concentrations. The growth inhibitory effects were observed by MTT assay and cell growth curve. The expression of TGF-β1 was detected by immunohistochemistry.
RESULTS AND CONCLUSION: Osthole could obviously inhibit the growth of human hypertrophic scar fibroblasts. MTT assay showed that osthole IC50 value toward hypertrophic scar fibroblasts was 15.2±2.0 μmol/L. Furthermore, the results of cell growth curve matched with the above results. Immunohistochemistry results showed that osthole could obviously inhibit TGF-β1 expression in the fibroblasts derived from hypertrophic scar tissue compared with the control group (P < 0.05). These findings suggest that osthole strongly inhibits the growth of human hypertrophic scar fibroblasts and decreases the expression of TGF-β1.

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