中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (41): 7677-7680.doi: 10.3969/j.issn.1673-8225.2011.41.019

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

滇南小耳猪正常胆道和胆道瘢痕来源成纤维细胞核转录因子κB和凋亡调控蛋白FLICE样抑制蛋白的表达

胡平海1，杨  慧2，李  立3   

  1. 1昆明医学院，云南省昆明市 650118
    2昆明医学院附属昆华医院，云南省昆明市 650032
    3昆明医学院附属甘美医院，云南省昆明市  650034
  • 收稿日期:2011-05-17 修回日期:2011-07-19 出版日期:2011-10-08 发布日期:2011-10-08
  • 作者简介:胡平海☆，男，1975年生，昆明医学院在读博士，主治医师，主要从事肝胆胰脾外科方面的研究。 hph1111@sina. com

Nuclear factor-kappa B and cellular Fas-associated death domain-like interleukin-1 beta-convening enzyme inhibitory protein expression in fibroblasts from normal bile duct and biliary scar tissue of Diannan small-eared pigs

Hu Ping-hai1, Yang Hui2, Li Li3   

  1. 1Kunming Medical College, Kunming  650118, Yunnan Province, China
    2Kunhua Hospital Affiliated to Kunming Medical University, Kunming  650032, Yunnan Province, China
    3Ganmei Hospital Affliated to Kunming Medical Unitersity, Kunming  650034, Yunnan Province, China
  • Received:2011-05-17 Revised:2011-07-19 Online:2011-10-08 Published:2011-10-08
  • About author:Hu Ping-hai☆, Studying for doctorate, Attending physician, Kunming Medical College, Kunming 650032, Yunnan Province, China hph1111@sina.com

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摘要：

背景：胆道瘢痕形成存在细胞的增殖和凋亡的平衡被破坏，尤其是成纤维细胞大量增殖并过度分泌胶原等基质成分。
目的：对比核转录因子κ B及其下游抗凋亡caspase-8同源结构FLICE抑制蛋白在胆道瘢痕及正常胆道来源的成纤维细胞系中的表达情况。
方法：8只滇南小耳猪随机等分为胆道损伤修复组和正常对照组，分别通过建立胆道损伤修复模型和不损伤胆道方法培养胆道瘢痕及正常胆道来源的成纤维细胞。
结果与结论：与正常胆道来源的成纤维细胞相比，胆道瘢痕来源的成纤维细胞中核转录因子κ B及凋亡调控蛋白FLICE样抑制蛋白表达的范围更广，表达更强。提示胆道损伤后核转录因子κ B激活增加，并导致caspase-8同源结构FLICE抑制蛋白过表达，从而导致胆道成纤维细胞凋亡减少致胆道瘢痕的形成。

关键词: 核转录因子&kappa, B, caspase-8同源结构FLICE抑制蛋白, 胆管, 成纤维细胞, 滇南小耳猪, 组织构建, 组织工程

Abstract:

BACKGROUND: Biliary scar formation results in destroyed balance between cell proliferation and apoptosis, in particular, fibroblasts greatly proliferate and excessively secrete collagen. 
OBJECTIVE: To compare nuclear factor-kappa B (NF-κB) and cellular Fas-associated death domain-like interleukin-1 beta-convening enzyme (FLICE) inhibitory protein expression in fibroblasts from normal bile duct and biliary scar tissue of Diannan small-eared pigs.
METHODS: Eight Diannan small-eared pigs were randomly divided into model group and normal control group. In the model group, biliary scar tissue-derived fibroblasts were harvested by establishing bile duct injury models. In the normal control group, normal bile duct fibroblasts were harvested.
RESULTS AND CONCLUSION: Compared with normal bile duct-derived fibroblasts, NF-κB and FLICE inhibitory protein expression was greater in the biliary scar tissue-derived fibroblast. These findings suggest that after biliary duct injury, NF-κB expression was increased and FLICE inhibitory protein was overexpressed, which leads to reduction in bile duct fibroblasts and finally results in biliary scar formation.

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