中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (7): 1215-1218.doi: 10.3969/j.issn.1673-8225.2011.07.016

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

脊髓全横断大鼠模型的构建

孟步亮1，巴迎春1，宋士娜2，陈姗姗2，李力燕2，王廷华2   

  1. 昆明医学院，1解剖学教研室，2神经科学研究所，云南省昆明市 650031
  • 收稿日期:2010-09-02 修回日期:2010-12-03 出版日期:2011-02-12 发布日期:2011-02-12
  • 通讯作者: 王廷华，博士，教授，昆明医学院神经科学研究所，云南省昆明市650031 tinghua_neuron@263.net
  • 作者简介:孟步亮☆，男，1975年生，山西省祁县人，汉族，1999年长治医学院毕业，讲师，昆明医学院在读博士，主要从事神经解剖学研究。 mbloso@126.com

Establishment of spinal cord transection injury models in rats

Meng Bu-liang1, Ba Ying-chun1, Song Shi-na2, Chen Shan-shan2, Li Li-yan2, Wang Ting-hua2   

  1. 1Department of Anatomy, 2Institute of Neuroscience, Kunming Medical College, Kunming  650031, Yunnan Province, China
  • Received:2010-09-02 Revised:2010-12-03 Online:2011-02-12 Published:2011-02-12
  • Contact: Wang Ting-hua, Doctor, Professor, Institute of Neuroscience, Kunming Medical College, Kunming 650031, Yunnan Province, China
  • About author:Meng Bu-liang☆, Studying for doctorate, Lecturer, Department of Anatomy, Kunming Medical College, Kunming 650031, Yunnan Province, China mbloso@126.com

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787

被引次数

17

摘要：

背景：脊髓全横断模型在造模时常难以保证神经纤维的完全离断。
目的：构建大鼠脊髓全横断损伤模型。
方法：将大鼠随机分为模型组和假手术组。模型组构建脊髓T10节段全横断模型；假手术组动物仅打开椎管与硬脊膜而后缝合，但不损伤脊髓。建模后1，3，5，7 d分别进行BBB评分以评估后肢运动功能，检测其体感诱发电位和运动诱发电位来评估神经传导通路的完整性，并行形态学观察来评估脊髓肉眼观病理形态。
结果与结论：与假手术组相比，模型组大鼠在建模后1，3，5，7 d时，其BBB评分降低(P < 0.01)，未检测出体感和运动诱发电位。形态学观察结果显示模型组大鼠脊髓完全横断，而假手术组脊髓形态完整。结果提示实验成功构建了大鼠脊髓全横断模型。

关键词: 脊髓损伤, 体感诱发电位, 运动诱发电位, 动物模型, 组织构建

Abstract:

BACKGROUND: The spinal cord injury (SCI) models reported in literature were short of determinacy and reliability.
OBJECTIVE: To establish an accurate and reliable rat spinal cord transection injury models.
METHODS: Rats were divided into model and sham-surgery groups at random. Model group prepared for T10 transection injury models; vertebral canal and spinal dura mater were exposed and sutured in the sham-surgery group. The motor function of hind limbs was evaluated by Basso, Beattie and Bresnahan (BBB) scores; the integrality of the nervous pathways was detected by somato-sensory evoked potential (SEP) and motor evoked potential (MEP), in addition, the pathologic and gross pathology of spinal cord were performed at 1, 3, 5 and 7 days after modeling.
RESULTS AND CONCLUSION: Compared with the sham-surgery group, the BBB scores of the model group were decreased at 1, 3, 5 and 7 days after modeling (P < 0.01). The SEP and MEP were not evoked out in the model group. The spinal cords were fully transected in the model group, which was intact in the sham-surgery group. that of. The result suggested that a spinal cord transection injury model is established successfully.

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