中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (2): 278-283.doi: 10.3969/j.issn.2095-4344.1515

• 生物材料综述 biomaterial review • 上一篇    下一篇

单细胞纳米包裹技术的应用:单个细胞催化、细胞保护与治疗

谢雯佳,王 剑,裴锡波   

  1. 四川大学华西口腔医院修复科,四川省成都市 610000
  • 收稿日期:2018-09-23 出版日期:2019-01-18 发布日期:2019-01-18
  • 通讯作者: 裴锡波,副教授,四川大学华西口腔医院口腔修复科,四川省成都市 610000
  • 作者简介:谢雯佳,女,1996年生,湖北省恩施市人,苗族,四川大学华西口腔医学院在读硕士,主要从事骨组织工程与骨再生研究。
  • 基金资助:

    国家自然科学基金青年基金(81601613,项目负责人:裴锡波)

Applications of single cell nanoencapsulation: single cell catalysis, cell protection and treatment

Xie Wenjia, Wang Jian, Pei Xibo   

  1. Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610000, Sichuan Province, China
  • Received:2018-09-23 Online:2019-01-18 Published:2019-01-18
  • Contact: Pei Xibo, Associate professor, Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610000, Sichuan Province, China
  • About author:Xie Wenjia, Master candidate, Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610000, Sichuan Province, China
  • Supported by:

    the National Natural Science Foundation of China (Youth Foundation), No. 81601613 (to PXB)

摘要:

文章快速阅读:

 

文题释义:
多聚电解质或其他材料的层层自组装技术:是一种包裹单个细胞形成有机纳米膜最常见的方法。层层自组装技术的作用原理是在适宜的模板上,通过基团间强相互作用力(静电相互作用)或弱相互作用力(氢键结合、疏水作用等),驱使目标化合物自发地在模版上形成结构完整、性能稳定、具有独特功能的薄膜。
纳米包裹:是应用极薄(厚度< 100 nm)纳米膜完成的一种包裹,近年来大量研究表明纳米膜除了能够包裹蛋白质、药物外,还能包裹单个活细胞。
 
 
背景:单细胞纳米包裹技术是用极薄(< 100 nm)纳米膜包裹单个细胞的一项新技术。被纳米包裹的细胞已被广泛应用于医学生物学研究等各个领域。
目的:综述单细胞纳米包裹技术的最新研究进展及应用,展望该技术未来的发展方向。
方法:利用计算机在PubMed、CNKI、万方数据库中进行相关文献检索,中英文检索关键词为“single cell,nano-coating,nano-encapsulation,cell-in-shell structure;单个细胞,纳米覆盖,纳米包裹”,最终纳入52篇文献进行综述。

结果与结论:多聚电解质的层层自组装技术具有过程简单、可调节,能形成性质多样的纳米膜等特征,已成为最广泛应用于合成单细胞外有机纳米膜的技术。应用多酚类物质的广泛黏附性及某些有机物能在细胞外自聚集的特性,也可形成性能较好的有机纳米包裹。除此之外,利用仿生矿化技术及利用某些无机物能直接在细胞表面结晶的性质,可在细胞表面形成无机纳米包裹。被纳米包裹的细胞已被广泛应用于单个细胞催化、细胞治疗、细胞保护、细胞生物学研究等各个领域。在未来的研究中,被包裹细胞的用途将更多地指导纳米膜材料的选择。

ORCID: 0000-0002-6634-7248(谢雯佳)

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

关键词: 单个细胞, 细胞包裹, 纳米膜, 纳米包裹, 纳米覆盖, 层层自组装技术, 生物材料

Abstract:

BACKGROUND: Single cell nanoencapsulation refers to coating single cells with ultrathin (< 100 nm) layers and it has been widely used in medical and biological areas.

OBJECTIVE: To review the development and applications of single cell nanoencapsulation.
METHODS: The literature search was performed in PubMed, CNKI and WanFang database, and the keywords were “single cell, nano-coating, nano-encapsulation, cell-in-shell structure” in English and Chinese, respectively. According to the inclusion and exclusion criteria, 52 articles were finally reviewed.

RESULTS AND CONCLUSION: The layer-by-layer deposition technique is most widely used due to their versatile processing and tunable properties. And many other materials, like polyphenols and surface-induced hydrogelator have been also utilized as organic cell-coating materials. Besides, bioinspired mineralization and crystallization were utilized for creating inorganic nanoshells. Single cell nanoencapsulation has been widely used in many important areas, including cell biocatalysis, cell therapy, cytoprotection and cell-based biology. Extensive adhesion of polyphenols and self-assembling of some organic materials have been also utilized to form nanoencapsulation of single cells. Besides, bioinspired mineralization and crystallization have been utilized for creating inorganic nanoshells. Nanoencapsulated single cells have been widely used in many important areas, including cell biocatalysis, cell therapy, cytoprotection and cell-based biology. The application of encapsulated cells will make a more important part in the selection of nanocoating materials in future research.

Key words: Nanostructures, Review, Tissue Engineering

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