中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (26): 4202-4207.doi: 10.3969/j.issn.2095-4344.0944

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

聚三亚甲基碳酸酯的体外降解性能

张 巍1,李红媛2,杨立群1,李 淼1,金 瑛1,易东旭1   

  1. 1辽宁省计划生育科学研究院,国家卫生和计划生育委员会生殖健康与遗传医学重点实验室,辽宁省沈阳市 110031;2中国医科大学附属第四医院,辽宁省沈阳市 110031
  • 收稿日期:2018-04-01
  • 通讯作者: 杨立群,博士,副研究员,辽宁省计划生育科学研究院,国家卫生和计划生育委员会生殖健康与遗传医学重点实验室,辽宁省沈阳市 110031
  • 作者简介:张巍,1981年生,河北省唐山市人,2007年吉林大学毕业,硕士,副研究员,主要从事生物医药高分子材料研究。
  • 基金资助:

    国家重点研发计划(2016YFC1000902);国家自然科学基金(51503093);辽宁省自然科学基金(20170540491);辽宁省博士科研启动基金(201501116);沈阳市科技攻关项目(F16-205-1-37)

Degradability of poly(trimethylene carbonate) in vitro

Zhang Wei1, Li Hong-yuan2, Yang Li-qun1, Li Miao1, Jin Ying1, Yi Dong-xu1   

  1. 1Liaoning Research Institute of Family Planning, Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, Shenyang 110031, Liaoning Province, China; 2the Fourth Affiliated Hospital of China Medical University, Shenyang 110031, Liaoning Province, China
  • Received:2018-04-01
  • Contact: Yang Li-qun, M.D., Associate researcher, Liaoning Research Institute of Family Planning, Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, Shenyang 110031, Liaoning Province, China
  • About author:Zhang Wei, Master, Associate researcher, Liaoning Research Institute of Family Planning, Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, Shenyang 110031, Liaoning Province, China
  • Supported by:

    the National Key Research and Development Program of China, No. 2016YFC1000902; the National Natural Science Foundation of China, No. 51503093; the Natural Science Foundation of Liaoning Province, No. 20170540491; the Doctoral Fund of Liaoning Province, No. 201501116; the Science and Technology Research Project of Shenyang, No. F16-205-1-37

摘要:

文章快速阅读:

 

文题释义:
输卵管栓塞:是采用微栓子对输卵管近端进行机械性栓塞,术中在宫腔镜直视下经阴道通过微导管将栓塞物送至输卵管间质部及峡部,阻断输卵管近端的通路完成栓塞,最早主要作为一种绝育或永久避孕手段。
生物降解:生物降解指材料在生物体内通过溶解、酶解及细胞吞噬等作用,在组织长入的过程中不断从体内排出,修复后的组织完全替代植入材料的位置,而材料在体内不存在残留的性质。
 
 
背景:目前输卵管栓塞器具因为金属质地存在着多种弊端,研究生物降解材料聚三亚甲基碳酸酯,在输卵管栓塞材料领域寻找突破点具有重要意义。
目的:观察聚三亚甲基碳酸酯的降解性能,论证其作为输卵管栓塞材料的可能性。
方法:选取不同相对分子质量的聚三亚甲基碳酸酯,分别浸泡在磷酸盐缓冲液和脂肪酶溶液中,37 ℃轻柔振动。定期观察试样的形态、失重率、数均相对分子质量和降解液的pH值,对聚三亚甲基碳酸酯的降解性能进行评价。

结果与结论:①聚三亚甲基碳酸酯的体外降解模式为表面溶蚀降解,降解产物不含酸性化合物;②脂肪酶能加速降解进程,但样品的形状和尺寸是影响相对分子质量下降速率的主要因素;③初始相对分子质量影响其降解速率和尺寸稳定性:相对分子质量越大,形状保持率越高,但质量损失越快,反之相对分子质量越小,质量损失越慢,但变形严重;④结果显示:选择适合的相对分子质量或通过共聚等手段调节其性能,聚三亚甲基碳酸酯有望用于输卵管栓塞材料。

ORCID: 0000-0003-0496-2613(张巍)

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

关键词: 聚三亚甲基碳酸酯, 体外降解, 脂肪酶, PBS, 输卵管积水, 生物材料, 药物控释材料, 输卵管栓塞, 溶蚀降解, 失重率

Abstract:

BACKGROUND: At present, the fallopian tube embolization devices have many malpractices because of the metal texture. Poly(trimethylene carbonate), a biodegradable material, is introduced to find a breakthrough in the field of tubal embolism materials.

OBJECTIVE: To observe the degradation behavior of poly(trimethylene carbonate) and to demonstrate the possibility of its use as a tubal embolization material.
METHODS: Poly(trimethylene carbonate) with different relative molecular weight was soaked in phosphate buffer and lipase solution at 37 oC with gently shaking. The degradation behavior of poly(trimethylene carbonate) was evaluated by observing the morphology, mass loss, relative molecular weight of the samples and the pH value of the degradation solution regularly.

RESULTS AND CONCLUSION: Poly(trimethylene carbonate) exhibited surface erosion degradation in vitro and the degradation products did not contain acid compounds. Lipase could accelerate the degradation process, but the shape and size of the samples were the main factors that affected the decline rate of molecular weight. The molecular weight of the samples affected the degradation rate and dimensional stability: the larger the relative molecular weight, the higher shape retention rate, but the faster mass loss; the smaller the relative molecular weight, the slower mass loss, but the more serious deformation. Therefore, poly(trimethylene carbonate) is expected to be used for fallopian tube embolization materials by selecting suitable molecular weight or adjusting its properties by copolymerization.

 

Key words: Biocompatible Materials, Biodegradation, Environmental, Fallopian Tubes

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