中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (16): 2558-2563.doi: 10.3969/j.issn.2095-4344.0829

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

小鼠尾断端愈合形态学观察及细胞增殖活跃区分析

郭景旭,王海涛,李树伟   

  1. 塔里木大学生命科学学院,塔里木盆地生物资源保护利用兵团重点实验室,新疆维吾尔自治区阿拉尔市  843300
  • 收稿日期:2017-12-03 出版日期:2018-06-08 发布日期:2018-06-08
  • 通讯作者: 李树伟,博士,教授,塔里木大学生命科学学院,塔里木盆地生物资源保护利用兵团重点实验室,新疆维吾尔自治区阿拉尔市 843300
  • 作者简介:郭景旭,男,1990年生,山东省嘉祥县人,汉族,塔里木大学生物化学与分子生物学在读硕士,主要从事动物生物化学与分子生物学研究。
  • 基金资助:

    国家自然科学基金(31560685)

Morphological observation of broken ends of the mouse tail during wound healing and analysis of cell proliferation active regions

Guo Jing-xu, Wang Hai-tao, Li Shu-wei   

  1. College of Life Sciences of Tarim University, Key Laboratory of Protection & Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Alar 843300, Xinjiang Uygur Autonomous Region, China
  • Received:2017-12-03 Online:2018-06-08 Published:2018-06-08
  • Contact: Li Shu-wei, M.D., Professor, College of Life Sciences of Tarim University, Key Laboratory of Protection & Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Alar 843300, Xinjiang Uygur Autonomous Region, China
  • About author:Guo Jing-xu, Master candidate, College of Life Sciences of Tarim University, Key Laboratory of Protection & Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Alar 843300, Xinjiang Uygur Autonomous Region, China
  • Supported by:

    the National Natural Science Foundation of China, No. 31560685

摘要:

文章快速阅读:

文题释义:
毛囊:在真皮乳头层里,毛根被内、外毛鞘包围,外毛鞘又被结缔组织细胞包围,形成口袋状,称毛囊。毛囊是包围在毛发根部的囊状组织,内层是上皮组织性毛囊,外层是结缔组织性毛囊,内层与表皮相连,外层则与真皮相连。毛囊由毛杆、毛凸、毛球、毛乳头、毛基质等部分组成。神经纤维末梢使毛囊具有感觉的功能,动脉和静脉毛细血管丛给毛囊提供血液。毛囊的最深处是位于角质层3-7 mm的毛乳头,它含有神经和血管,向毛杆提供养分。毛囊的发育经过表皮和真皮之间一系列复杂的相互作用而形成,而具有周期性。
皮肤源性前体细胞:是一种神经嵴来源的前体细胞,具有来源丰富、易于获取和多向分化的潜能等优点,能够分化为在体内外都有功能的许旺细胞、骨样细胞和胰岛素样细胞等多种细胞类型。其在多种组织修复和疾病治疗,特别是神经损伤再修复中显示了良好的应用前景。
摘要
背景:
皮肤创面中起到修复再生作用的细胞主要为皮肤源性前体细胞,当皮肤创面发生后,皮肤中的皮肤源性前体细胞进行分裂增殖,迁移修复创面,尽可能恢复损伤处原有皮肤的生理及美观功能。
目的:观察小鼠尾部结构以及离断后断端愈合形态,初步探讨这些特征与小鼠尾部皮肤创伤修复的关系。
方法:利用眼科显微外科剪对2日龄昆明小鼠尾部尖端进行人工统一离断,建立鼠尾断端毛囊再生动物模型,定位皮肤创面愈合过程中细胞增殖活跃区域,定期获取小鼠尾部皮肤创面愈合样品,OCT包埋。常规病理染色观察创面愈合形态;对鼠尾细胞增殖进行免疫荧光标记,利用免疫荧光染色鉴定小鼠尾部愈合部位皮肤激活蛋白AP-1的表达。
结果与结论:①鼠尾结构复杂,血运丰富,小鼠尾尖离断后可以达到较好愈合形态;②鼠尾部位皮肤细胞的增殖活跃主要在毛囊部位以及真皮乳头层进行细胞分裂,参与修复创面与再生;③荧光染色发现激活蛋白AP-1的表达主要集中在愈合皮肤的表皮层和真皮乳头层;④结果说明愈合处的皮肤表皮层和真皮乳头层具有较强的再生能力。

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
ORCID: 0000-0002-3701-505X(郭景旭)

关键词: 组织构建, 皮肤, 鼠尾, 创面愈合, 毛囊再生, 细胞增殖, 免疫荧光, EdU, AP-1

Abstract:

BACKGROUND: Skin-derived precursor cells play a regenerative role in wound healing, which can restore the physiological and aesthetic features of the skin by mitosis, proliferation and migration to the injury region.
OBJECTIVE: To observe the structure of the mouse tail and the healing of the broken end, and then to investigate the correlation of these characteristics with the repair of skin trauma at the tail.
METHODS: The tails of 2-day-old Kunming mice were cut off using ophthalmic microsurgical scissors to establish the mouse model of hair follicle regeneration in the tail. Regions in which the cells proliferated actively were determined. Wound healing samples were regularly taken, and embedded using OCT. Wound healing of the broken end was routinely observed by pathological staining. Cell proliferation was observed by immunofluorescence. Expression of AP-1 in the healing site was identified by immunofluorescent staining.
RESULTS AND CONCLUSION: The structure of the mouse tail was complex, with rich blood supply. Good healing was observed in the broken end of the mouse tail. The cells in the hair follicles and dermal papillary layer showed active proliferation and mitosis, and thereby participated in wound repair and regeneration. Fluorescence staining results found that the positive expression of AP-1 was mainly concentrated in the epidermis and dermal papilla. These findings indicate that the epidermis and dermal papilla possess a strong ability to regenerate.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words: Skin, Cell Proliferation, Wound Healing, Transcription Factors, Tissue Engineering

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