中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (18): 2921-2926.doi: 10.3969/j.issn.2095-4344.0766

• 生物材料综述 biomaterial review • 上一篇    下一篇

磁性纳米颗粒细胞内吞评价方法研究及进展

王润生1,2,刘义灏3,毛克亚1   

  1. 1解放军总医院骨科,北京市 100853;2广西中医药大学第三附属医院骨科,广西壮族自治区柳州市 545001;3江苏大学,江苏省镇江市 212013
  • 收稿日期:2018-01-05 出版日期:2018-06-28 发布日期:2018-06-28
  • 通讯作者: 毛克亚,主任医师,解放军总医院骨科,北京市 100853
  • 作者简介:王润生,男,1985年生,安徽省六安市人,汉族,解放军医学院在读博士,主治医师,主要从事脊柱外科、骨生物材料研究。
  • 基金资助:

    国家自然科学基金(51772328)

Evaluation methodology of cellular uptake of magnetic nanoparticles

Wang Run-sheng1, 2, Liu Yi-hao3, Mao Ke-ya1   

  1. 1Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China; 2Department of Orthopedics, Third Affiliated Hospital of Guangxi University of Chinese Medicine, Liuzhou 545001, Guangxi Zhuang Autonomous Region, China; 3Jiangsu University, Zhenjiang 212013, Jiangsu Province, China
  • Received:2018-01-05 Online:2018-06-28 Published:2018-06-28
  • Contact: Mao Ke-ya, Chief physician, Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China
  • About author:Wang Run-sheng, Doctoral candidate, Attending physician, Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China; Department of Orthopedics, Third Affiliated Hospital of Guangxi University of Chinese Medicine, Liuzhou 545001, Guangxi Zhuang Autonomous Region, China

摘要:

文章快速阅读:

 

文题释义:
磁性纳米颗粒:是一类智能型的纳米磁性材料,既具有纳米材料所特有的性质如粒径小、比表面积大、偶连容量高,又具有磁响应性及超顺磁性,可在恒定磁场下聚集和定位,在交变磁场下吸收电磁波产热,进行肿瘤靶向细胞内热疗;由于其高比表面积,经表面修饰后可连接化疗药物、放射性元素,特定的分子或配体,与肿瘤细胞能靶向结合,在内源性或外源性因素激发下,发挥靶向化疗、放疗,又由于其核具有产热效能,可同时发挥热化疗、热放疗作用。
细胞内吞作用:又称入胞作用或胞吞作用,是通过质膜的变形运动将细胞外物质转运入细胞内的过程。根据入胞物质的不同大小,以及入胞机制的不同可将内吞作用分为3种类型:吞噬作用、吞饮作用、受体介导的内吞作用。
 
 
背景:磁性纳米颗粒在肿瘤热疗、药物可控性释放、磁共振造影等领域具有广阔应用前景,生物材料与细胞的作用关系是体外研究的重要内容,磁性纳米颗粒细胞内吞评价是肿瘤热疗、药物可控性释放不可或缺的环节。
目的:综述磁性纳米颗粒细胞内吞评价方法。
方法:由第一作者应用计算机检索PubMed、SCI、Embase数据库2010年1月至2017年6月关于磁性纳米颗粒细胞内吞的文章,磁性纳米颗粒选择主题词“Magnetic nanoparticles”,细胞内吞选择关键词“Cellular uptake”或“Internalization”或“Endocytosis”,两者采用逻辑运算符“AND”连接。

结果与结论:磁性纳米颗粒细胞内吞的评价有定性和定量评估方法,其中定性评估方法包括普鲁士蓝染色、荧光显微镜、共聚焦显微镜、透射电镜、光热显微镜、原子力显微镜;定量评估方法包括电感耦合等离子体、磁化强度测定、普鲁士蓝染色比色法。现有评估方法逐渐由定性评价方法向定量评价方法发展,每种方法具有各自优缺点,评价的角度也不同,根据磁性纳米颗粒功能、用途差异选择不同的评价方法或联合运用,才能为其在生物医学中的应用提供可靠的实验依据。

ORCID: 0000-0003-1547-3460orcid.org/0000-0003-1547-3460(王润生)

关键词: 磁性纳米颗粒, 细胞内吞, 定性评估, 定量评估, 国家自然科学基金, 生物材料

Abstract:

BACKGROUND: Magnetic nanoparticles have attracted tremendous attention for their diverse biomedical applications involving tumor hyperthermia, drug controlled release and magnetic resonance angiography. The interaction between biomaterials and cells is the major part in the in vitro study, and the evaluation of cellular

uptake of magnetic nanoparticles is indispensable for tumor hyperthermia and drug delivery.
OBJECTIVE: To review the progress of evaluation methodology of cellular uptake of magnetic nanoparticles.
METHODS: The articles about magnetic nanoparticles and cellular uptake were retrieved from PubMed, SCI and Embase databases published during January 2010 to June 2017 by the first author using computer. The Mesh terms were “magnetic nanoparticles”, and the key words were “cellular uptake”, “internalization” or “endocytosis”. The logical operator between them was “AND”.

RESULTS AND CONCLUSION: Qualitative quantitative assessment methods for cellular uptake of magnetic nanoparticles include Prussian blue staining, fluorescence microscopy, confocal microscopy,transmission electron microscopy. Quantitative assessment methods for cellular uptake of magnetic nanoparticles include inductively coupled plasma, magnetization measurements, colorimetry of Prussian blue staining. The development direction of the evaluation methodology is from qualitative to quantitative. As each method has its own merits and demerits, we should choose the right evaluation method based on the function and application of magnetic nanoparticles and then supply the right experimental basis for the biomedical application of magnetite nanoparticles.

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