中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (13): 1981-1986.doi: 10.3969/j.issn.2095-4344.0495

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

明胶纤维支架促进人牙髓干细胞的成纤维分化

姜力铭1，2，夏 商1，宋 戈1，陈 旭1，2   

  1. 1中国医科大学口腔医学院，辽宁省沈阳市 110002；2辽宁省口腔疾病重点实验室，辽宁省沈阳市 110002
  • 修回日期:2018-01-26 出版日期:2018-05-08 发布日期:2018-05-08
  • 通讯作者: 陈旭，博士，教授，中国医科大学口腔医学院，辽宁省沈阳市 110002
  • 作者简介:姜力铭，女，1983年生，辽宁省庄河县人，汉族，2014年吉林大学口腔医学专业毕业，博士，讲师，主治医师，主要从事牙齿再植和牙周组织再生相关研究工作。
  • 基金资助:

    辽宁省教育厅科学研究项目(LS201610)；中国医科大学口腔医学院青年科技基金启动项目(K101593-15-05)

Gelatin fibrous scaffolds promote fibrogenic differentiation of human dental pulp stem cells

Jiang Li-ming1, 2, Xia Shang1, Song Ge1, Chen Xu1, 2   

  1. 1School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China; 2Liaoning Province Key Laboratory of Oral Disease, Shenyang 110002, Liaoning Province, China
  • Revised:2018-01-26 Online:2018-05-08 Published:2018-05-08
  • Contact: Chen Xu, M.D., Professor, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China; Liaoning Province Key Laboratory of Oral Disease, Shenyang 110002, Liaoning Province, China
  • About author:Jiang Li-ming, M.D., Lecturer, Attending physician, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China; Liaoning Province Key Laboratory of Oral Disease, Shenyang 110002, Liaoning Province, China
  • Supported by:

    the Scientific Research Project of Liaoning Province Education Department, No. LS201610; Young Science and Technology Foundation Startup Project of School of Stomatology, China Medical University, No. K101593-15-05

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摘要：

文章快速阅读：

文题释义：
明胶：是通过水解作用从动物体内胶原中分离加工得到的变性胶原，通过不同的预处理方式可以获得A型和B型两种明胶。处理过程显著降低了明胶的抗原性，另因其具有良好的生物相容性和生物降解性，被广泛应用于药物释放和组织工程领域。
牙髓干细胞：是一种位于牙髓组织内的间充质干细胞，于2000年被Gronthos研究组发现并报道。牙髓干细胞具有较强的增殖、自我更新和多向分化能力，是一种可用于组织工程和再生医学的新型干细胞来源。

 

摘要
背景：牙髓炎、牙髓坏死使得牙齿丧失活力，牙质变脆易折裂，并且失去牙髓免疫防御反应，如何实现牙髓组织再生成为口腔领域研究热点。支架材料的理化性质、生物相容性等对于干细胞的增殖和分化起关键作用。
目的：探讨明胶纤维支架是否能够促进牙髓干细胞向成纤维细胞分化。
方法：通过静电纺丝法合成不同浓度的明胶纤维支架，利用扫描电镜观察支架的表面形貌，拉伸实验检测明胶纤维支架的物理学性质。将人牙髓干细胞接种于支架材料表面，利用MTT和RT-PCR方法检测明胶纤维支架对人牙髓干细胞增殖和成纤维分化能力的影响。
结果与结论：①7.5%的明胶纤维支架纤维直径为(2.02±0.36) μm，交联后纤维直径增大，为(3.15±0.52) μm；15%的明胶支架纤维出现粘结，交联后粘结情况更加显著，纤维结构丧失，呈现平面结构；②两种明胶纤维支架均能促进人牙髓干细胞的黏附和生长，第7天时，7.5%明胶纤维支架上的细胞数量明显高于15%明胶纤维支架(P < 0.05)；③7.5%明胶纤维支架组Collagen Ⅰ、α-SMA、Periostin和Fibronectin的表达水平高于15%明胶纤维支架组(P < 0.05)；④结果表明，7.5%明胶支架的多孔纤维结构更利于细胞的增殖和向成纤维细胞分化。

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程
ORCID: 0000-0002-2158-8320(陈旭)

关键词: 牙髓干细胞, 明胶, 纤维支架, 静电纺丝, 细胞增殖, 成纤维分化, 干细胞

Abstract:

BACKGROUND: A tooth can be led to lose viability, split easily and miss immune defensive response by pulpitis and pulp necrosis. Determining how to achieve dental pulp regeneration has become a research focus in dentistry. The physicochemical properties and biocompatibility of scaffold materials are crucial for proliferation and differentiation of stem cells.
OBJECTIVE: To study whether a gelatin scaffold can induce dental pulp stem cells to differentiate into fibroblasts.
METHODS: Gelatin scaffolds at different concentrations were prepared by electrospinning method. The surface morphology and physical properties of gelatin scaffolds were tested by using scanning electron microscope and tensile tests. The human dental pulp stem cells (hDPSCs) were seeded on the scaffolds and the cell proliferation and fibrogenic differentiation were tested using MTT and RT-PCR.
RESULTS AND CONCLUSION: The fiber diameter of the 7.5% gelatin scaffold was (2.02±0.36) μm, and it was increased to (3.15±0.52) μm after cross-linking. In the 15% gelatin scaffold, fiber bonding was detected and strengthened until the emergence of flat structures after cross-linking. Both 7.5% and 15% gelatin scaffolds could promote the adhesion and growth of hDPSCs. On day 7, the cell number on the 7.5% gelatin scaffold was significantly higher than that on the 15% gelatin scaffold (P < 0.05). The levels of Collagen I, α-SMA, Periostin and Fibronectin were also higher in the 7.5% gelatin scaffold than in the 15% gelatin scaffold (P < 0.05). In conclusion, 7.5% gelatin scaffold is more beneficial to the proliferation and fibrogenic differentiation of hDPSCs.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Gelatin, Cross-Linking Reagents, Dental Pulp, Stem Cells, Tissue Engineering

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