中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (46): 6956-6956.doi: 10.3969/j.issn.2095-4344.2016.46.016

• 组织构建综述 tissue construction review • 上一篇    下一篇

骨组织工程中的间质干细胞：成骨活性、免疫特性、促血管化及成骨效应

陈震东1，徐房添2，高  辉2   

  1. 1赣南医学院，江西省赣州市  341000；2赣南医学院第一临床医学院，江西省赣州市  341000
  • 收稿日期:2016-08-24 出版日期:2016-11-11 发布日期:2016-11-11
  • 通讯作者: 高辉，博士，教授，赣南医学院第一临床医学院，江西省赣州市
  • 作者简介:陈震东，男，1989年生，江西省赣州市人，汉族，赣南医学院2014级临床医学在读硕士，主要从事骨关节外科方面研究。
  • 基金资助:

    国家自然科学基金(8156090191)

Mesenchymal stem cells in bone tissue engineering: osteogenic activity, immunogenicity, proangiogensis and osteogenesis

Chen Zhen-dong1, Xu Fang-tian2, Gao Hui2   

  1. 1Gannan Medical University, Ganzhou 341000, Jiangxi Province, China; 2First Clinical Institute of Gannan Medical University, Ganzhou 341000, Jiangxi Province, China
  • Received:2016-08-24 Online:2016-11-11 Published:2016-11-11
  • Contact: Gao Hui, M.D., Professor, First Clinical Institute of Gannan Medical University, Ganzhou 341000, Jiangxi Province, China
  • About author:341000Chen Zhen-dong, Studying for master’s degree, Gannan Medical University, Ganzhou 341000, Jiangxi Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 8156090191

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摘要：

文章快速阅读：


文题释义：
免疫调节：是指免疫系统中的免疫细胞和免疫分子之间，以及与其他系统如神经内分泌系统之间的相互作用，使得免疫应答以最恰当的形式维持在最适当的水平。
成骨细胞：是骨形成的主要功能细胞，负责骨基质的合成、分泌和矿化。骨不断地进行着重建，骨重建过程包括破骨细胞贴附在旧骨区域，分泌酸性物质溶解矿物质，分泌蛋白酶消化骨基质，形成骨吸收陷窝；其后，成骨细胞移行至被吸收部位，分泌骨基质，骨基质矿化而形成新骨。破骨与成骨过程的平衡是维持正常骨量的关键。
摘要
背景：临床治疗由创伤、感染、肿瘤以及先天性疾病导致的骨缺损仍然是一个挑战。骨组织工程的研究，为骨缺损的治疗提供了一种新的解决办法，对治疗骨缺损具有重要的指导意义。
目的：回顾近年来间质干细胞在骨组织工程中的增殖和成骨活性、免疫特性、促血管化以及在体实验的成骨效果的研究进展。
方法：检索万方数据库和PubMed数据库2008年至2016年文献，检索词分别为“间质干细胞、组织工程、成骨、免疫、血管化”和“mesencgymal stem cell，tissueengineering，osteogenesis，immuneproperty，angiogenesis”。纳入与间质干细胞、组织工程、成骨、免疫及血管化相关的研究，排除重复及陈旧文献。共检索到1 772篇文章，按纳入和排除标准文献进行筛选，共纳入41篇文章进行综述分析。
结果与结论： 在骨组织工程中，骨髓间质干细胞与脂肪间质干细胞应用较为广泛。研究认为骨髓间质干细胞成骨活性高于脂肪间质干细胞。间质干细胞具有明显的免疫调节和组织修复能力，可减少损伤部位炎症反应，加快组织修复。间质干细胞免疫调节作用不只有免疫抑制作用，也有免疫促进作用，脂肪间质干细胞比骨髓间质干细胞具有较强的免疫调节性。低氧培养下的脂肪间质干细胞能够分泌更高的促血管生成因子，生成更多的血管结构。实验研究证实聚乳酸多孔纳米材料结合纳米碳生物材料可以明显促进骨髓间质干细胞的增殖和成骨。由于间质干细胞具有生物向性，可能成瘤分化，故间质干细胞的临床应用一直持谨慎态度。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0001-8521-307X(陈震东)

关键词: 组织构建, 骨组织工程, 间质干细胞, 增殖, 成骨, 免疫, 血管化, 国家自然科学基金

Abstract:

BACKGROUND: It is still a challenge to repair bone defects caused by trauma, infection, tumor and congenital diseases. Bone tissue engineering is a promising method for bone defect repair showing important guiding significance in the clinic.
OBJECTIVE: To review the progress of proliferation, osteogenic activity, immunogenicity, proangiogensis and in vivo osteogenic effect of mesenchymal stem cells in bone tissue engineering.
METHODS: The first author retrieved Wanfang and PubMed datebase for literatures published from 2008 to 2016, using the keywords of “mesenchymal stem cell, tissue engineering, osteogenesis, immune property, angiogenesis” in Chinese and English, respectively. Articles regarding mesenchymal stem cells, tissue engineering, osteogenesis, immune property and angiogenesis were included, and repetitive and dated studies were excluded. Totally 1 772 articles were retrieved initially, and in accordance with inclusion and exclusion criteria, 41 eligible articles were included for review analysis.
RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells and adipose-derived mesenchymal stem cells are extensively applied in bone tissue engineering. Studies have shown that the osteogenic activity of bone marrow mesenchymal stem cells is higher than that of adipose-derived mesenchymal stem cells, but its immune regulation effect is weaker than that of adipose-derived mesenchymal stem cells. Mesenchymal stem cells hold remarkable immune regulation (immunosuppression and immune enhancement) and tissue repair capacity, which can eliminate inflammatory reactions at injured sites, promoting tissue repair. Adipose-derived mesenchymal stem cells cultured under hypoxia environment can secret more angiogenic cytokines generating more vascular structures. Furthermore, increasing proof have confirmed that porous nano-polylactic acid combined with nano-carbon biological material can significantly promote the proliferation and osteogenesis of bone marrow mesenchymal stem cells. Taking its tumorigenesis into consideration, mesenchymal stem cells should be prudently used in the clinic.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Mesenchymal Stem Cells, Bone and Bones, Immunity, Tissue Engineering

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