中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (19): 2844-2849.doi: 10.3969/j.issn.2095-4344.2016.19.016

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

角膜缘干细胞缺乏是否可导致归巢因子基质细胞衍生因子1水平变化

张  伟1,黄文志1,张玉平2   

  1. 广州市第一人民医院,1老年病科眼科,2血液病科,广东省广州市 510180
  • 收稿日期:2016-03-22 出版日期:2016-05-06 发布日期:2016-05-06
  • 通讯作者: 张玉平,博士,主任医师,广州市第一人民医院血液病科,广东省广州市 510180
  • 作者简介:张伟,女,1975年生,安徽省人,汉族,博士,副主任医师。
  • 基金资助:

    国家自然科学青年科学基金项目(81300733)

Variation of stromal cell-derived factor-1 level in a limbal stem cell deficiency model

Zhang Wei1, Huang Wen-zhi1, Zhang Yu-ping2   

  1. 1Department of Geriatrics Ophthalmology, 2Department of Hematology, First People’s Hospital of Guangzhou, Guangzhou 510180, Guangdong Province, China
  • Received:2016-03-22 Online:2016-05-06 Published:2016-05-06
  • Contact: Zhang Yu-ping, M.D., Chief physician, Department of Hematology, First People’s Hospital of Guangzhou, Guangzhou 510180, Guangdong Province, China
  • About author:Zhang Wei, M.D., Associate chief physician, Department of Geriatrics Ophthalmology, First People’s Hospital of Guangzhou, Guangzhou 510180, Guangdong Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81300733

摘要:

文章快速阅读:

文题释义:
角膜缘干细胞缺乏:
此类患者患者角膜上皮彻底失去再生和修复能力,对视力造成严重危害,由其所导致的失明在角膜盲中占据相当的比例,但是由于角膜材料的短缺,导致治疗上非常棘手,目前的研究表明干细胞移植重建眼表功能将成为除角膜移植之外极具希望前景的治疗方案。
基质细胞衍生因子1:趋化因子CXCL12 又称基质细胞衍生因子1,是小分子的细胞因子,属于趋化因子蛋白家族。趋化因子CXCL12对淋巴细胞有强烈的趋化作用并在发育中起重要作用。

摘要
背景:
如何提高细胞移植的效率,促进来源有限、数量稀缺的角膜缘干细胞走向组织修复的第一步即归巢,是提高移植效率的关键。在视网膜色素上皮细胞的损伤修复中基质细胞衍生因子1可发挥着重要作用。
目的:观察大鼠及小鼠眼局部角膜缘干细胞缺乏是否会引起外周血中归巢因子基质细胞衍生因子1质量浓度的变化,以期明确角膜缘干细胞缺乏是否会启动外周血中该因子产生变化,从而吸引外周血干细胞向角膜方向归巢。
方法:剖切法制作大鼠和小鼠角膜缘干细胞缺乏模型眼,在为期1个月的观察期内连续抽取外周血,ELISA法检测基质细胞衍生因子1质量浓度。
结果与结论:①大鼠外周血基质细胞衍生因子1质量浓度:在建模后24 h即发生变化,呈增长态势,在建模后第3天达到增长峰值,其后逐渐下降,但在第25天又出现1个增长峰值,较第3天的峰值低,其后恢复至正常数值;②小鼠外周血中基质细胞衍生因子1质量浓度:在建模后12 h即发生变化,呈增长态势,在建模后第3天达到增长峰值,其后逐渐下降,但在第20天又出现1个增长峰值,较第3天的峰值低,其后恢复至正常数值;③结果说明:外周血中基质细胞衍生因子1水平与角膜缘干细胞缺乏造成的损伤刺激存在一定关联,可能在干细胞迁移修复角膜损伤中起重要的作用。

 

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
ORCID:
0000-0003-1055-2071(张玉平)

关键词: 干细胞, 分化, 角膜缘干细胞, 角膜缘干细胞缺乏, 外周血, 基质细胞衍生因子1, 归巢因子, ELISA, 大鼠, 小鼠, 国家自然科学基金

Abstract:

BACKGROUND: How to promote homing of scarce limbal stem cells is the key to improving transplantation efficiency. And stromal cell-derived factor-1 plays an important role in the repair of retinal pigment epithelial cells injury.
OBJECTIVE: To observe changes of stromal cell-derived factor-1 levels in rat and mouse limbal stem cell deficiency models in order to confirm whether these changes will induce peripheral blood stem cells homing into the cornea.
METHODS: Eye models of limbal stem cell deficiency were prepared in rats and mice by cutting method, then peripheral blood samples were continuously drawn to detect the mass concentration of stromal cell-derived factor-1 by enzyme-linked immunosorbent assay within 1 month after modeling.
RESULTS AND CONCLUSION: In the rat peripheral blood, the mass concentration of stromal cell-derived factor-1 started to increase at 24 hours after modeling and firstly peaked at the 3rd day; then it tended to decrease and reached a second peak lower than the first one at the 25th day; subsequently, it returned to the normal level. In the meanwhile, the mass concentration of stromal cell-derived factor-1 in the mouse peripheral blood, increased at 12 hours after modeling, peaked at the 3rd day, subsequently decreased until another peak lower than the former one presented at the 20th day, and finally it returned to the normal level. In conclusion, the level of stromal cell-derived factor-1 in the peripheral blood is significantly associated with limbal stem cells deficiency, which may play an important role in corneal injury repair.

 

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Stem Cells, Tissue Engineering, Cornea

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