中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (6): 891-897.doi: 10.3969/j.issn.2095-4344.2016.06.019

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

碱性成纤维细胞生长因子及胰岛素样生长因子影响骨髓间充质干细胞增殖及胶原合成

徐海龙1，丁 越1，谢 洪1，孙晓菊1，谢慧心2   

  1. 1辽宁省人民医院，辽宁省沈阳市 110016；2中国医科大学研究生院，辽宁省沈阳市 110001
  • 收稿日期:2015-12-19 出版日期:2016-02-05 发布日期:2016-02-05
  • 通讯作者: 丁越，硕士，主任医师，辽宁省人民医院，辽宁省沈阳市 110016
  • 作者简介:徐海龙，男，1980年生，黑龙江省延寿县人，汉族，2005年辽宁医学院毕业，主治医师，主要从事口腔外科方面工作。
  • 基金资助:
    辽宁省科学技术计划项目(2013225021)，“恶性肿瘤转化医学研究中心及协同研究网络建设”

Effect of basic fibroblast growth factor and insulin-like growth factor-1 on proliferation and collagen synthesis of bone marrow mesenchymal stem cells

Xu Hai-long1, Ding Yue1, Xie Hong1, Sun Xiao-ju1, Xie Hui-xin2   

  1. 1the People’s Hospital of Liaoning Province, Shenyang 110016, Liaoning Province, China; 2Graduate School of China Medical University, Shenyang 110001, Liaoning Province, China
  • Received:2015-12-19 Online:2016-02-05 Published:2016-02-05
  • Contact: Ding Yue, Master, Chief physician, the People’s Hospital of Liaoning Province, Shenyang 110016, Liaoning Province, China
  • About author:Xu Hai-long, Attending physician, the People’s Hospital of Liaoning Province, Shenyang 110016, Liaoning Province, China
  • Supported by:

    the Science and Technology Plan Project of Liaoning Province, No. 2013225021

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507

被引次数

4

摘要：

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文题释义：

碱性成纤维细胞生长因子：主要存在于表皮基底的角质形成细胞内，是重要的促细胞有丝分裂因子和血管生成因子，对来源于中胚层和神经外胚层的多种类型细胞具有广泛的生物学活性，能促进细胞增殖、生长、分化，促进血管新生，改善细胞生活微环境，促进受损神经再生及血管、肌肉、皮肤等的修复。
胰岛素样生长因子1：是由70个氨基酸组成的具有内分泌、自分泌及旁分泌特性的单链多肽，分子质量约为7.5 ku，主要由肝细胞合成和分泌，通过其类胰岛素样作用，促进组织摄取葡萄糖，增加细胞对氨基酸和糖原的吸收，刺激糖异生和糖酵解进而促进蛋白质和脂肪合成，对机体生长发育、免疫调节起着重要调节作用。

 

背景：在皮肤修复及瘢痕形成过程中如何控制胶原蛋白的有序形成是值得关注的热点。
目的：探讨碱性成纤维细胞生长因子与胰岛素样生长因子1对体外培养大鼠骨髓间充质干细胞增殖及胶原合成的影响。
方法：体外分离培养大鼠骨髓间充质干细胞，进行成脂及成骨分化鉴定，在第3代细胞培养液中分别添加碱性成纤维细胞生长因子、胰岛素样生长因子1以及两者联合干预，以加入正常培养基为对照组。培养12，24，48，72，96 h时采用MTT检测细胞增殖情况，培养10 d时采用RT-PCR检测Ⅰ型胶原、Ⅲ型胶原mRNA表达，Western Blot检测Ⅰ型胶原、Ⅲ型胶原蛋白表达。

结果与结论：①与对照组比较，单独应用碱性成纤维细胞生长因子可显著促进骨髓间充质干细胞增殖，抑制Ⅰ型胶原、Ⅲ型胶原表达。②单独应用胰岛素样生长因子1可显著促进骨髓间充质干细胞增殖，促进Ⅰ型胶原、Ⅲ型胶原表达。③两种生长因子联用相比单独应用，促进增殖效果更为显著，同时使Ⅰ型胶原、Ⅲ型胶原表达趋于正常水平。④结果表明碱性成纤维细胞生长因子、胰岛素样生长因子1联合应用在促进骨髓间充质干细胞增殖的同时能够对Ⅰ型胶原、Ⅲ型胶原的表达有所限制，可能有助于控制瘢痕形成及促进创伤愈合。 

ORCID: 0000-0001-5344-7090 (徐海龙)

关键词: 干细胞, 骨髓干细胞, 碱性成纤维细胞生长因子, 胰岛素样生长因子1, 骨髓间充质干细胞, 细胞增殖, Ⅰ型胶原, Ⅲ型胶原

Abstract:

BACKGROUND: How to control the orderly formation of collage in skin repair and scarring process is worthy of attention.
OBJECTIVE: To investigate the effect of basic fibroblast growth factor (bFGF) combined with insulin-like growth factor 1 (IGF-1) on the proliferation and collagen synthesis of rat bone marrow mesenchymal stem cells in vitro.
METHODS: Rat bone marrow mesenchymal stem cells were isolated and cultured to induce adipogenic differentiation assessed by oil red O staining and osteogenic differentiation identified by alizarin red staining in vitro. Passage 3 cells were cultured in the medium containing bFGF, IGF-1, combination of them or the control fluid, respectively. MTT assay was used to detect cell proliferation at 12, 24, 48, 72 and 96 hours of culture. The expression of type I collagen and type III collagen were detected by RT-PCR and western blot after 10 days of incubation.
RESULTS AND CONCLUSION: Compared with the control group, bFGF or IGF-1 alone significantly promoted the proliferation of bone marrow mesenchymal stem cells, and inhibited the expression of type I collagen and type III collagen. After combined use of bFGF and IGF-1, the proliferation of bone marrow mesenchymal stem cells was improved more significantly, and the expression of type I collagen and type III collagen returned to normal levels. These findings indicate that the combination of IGF-1 and bFGF can promote proliferation of bone marrow mesenchymal stem cells and restrain the expression of type I collagen and type III collagen, which may be helpful for control and repair of scar formation during wound healing.