中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (28): 4550-4556.doi: 10.3969/j.issn.2095-4344.0330

• 组织构建综述 tissue construction review • 上一篇    下一篇

不同膝骨关节炎模型兔的构建方法：合理选择及特点

彭  旭，熊华章，杨继滨，尤  奇，金  瑛，张  骏，葛  振，刘  毅   

  1. 遵义医学院附属医院骨一科，贵州省遵义市   563000
  • 收稿日期:2018-04-28 出版日期:2018-10-08 发布日期:2018-10-08
  • 通讯作者: 刘毅，教授，硕士生导师，遵义医学院附属医院骨一科，贵州省遵义市 563000
  • 作者简介:彭旭，男，1985年生，四川省遂宁人，汉族，遵义医学院在读硕士，主要从事关节退变和运动医学的研究。
  • 基金资助:

    贵州省科学技术基金资助项目(黔科合LH字[2016]7477号)

Different methods for establishing rabbit models of knee osteoarthritis: rational option and characters

Peng Xu, Xiong Hua-zhang, Yang Ji-bin, You Qi, Jin Ying, Zhang Jun, Ge Zhen, Liu Yi   

  1. First Department of Orthopedics, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China
  • Received:2018-04-28 Online:2018-10-08 Published:2018-10-08
  • Contact: Liu Yi, Professor, Master’s supervisor, First Department of Orthopedics, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China
  • About author:Peng Xu, Master candidate, First Department of Orthopedics, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China
  • Supported by:

    the Science and Technology Foundation of Guizhou Province, No. LH [2016]7477

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摘要：

文章快速阅读：

文题释义：
Hulth法：为经典骨关节炎造模方式，手术需切除前、后交叉韧带、内侧副韧带及内侧半月板，造成关节的极度不稳定，从而诱发骨关节炎改变，该方法诱导率高，稳定性好，但创伤大，感染风险较高，产生的炎症因子对早期骨关节炎软骨及滑膜的生化变化影响较大。
改良Hulth法：是在传统Hulth法的基础上进行改良，是在切除前交叉韧带及基础上进一步破坏关节内的稳定结构，如再全部或部分切除内外侧半月板、再切断内侧副韧带等，具有创伤较小，稳定好，重复性好的特点。
摘要
背景：动物模型作为骨关节炎的研究现已广泛运用，其中兔是最常使用的模型，在众多的建模方法中，诱导模型因其稳定、高效、可重复的特点，成为运用最多的方法。
目的：对近年国内外兔膝骨关节炎诱导模型的研究现状、应用进行分析。
方法：应用计算机检索PubMed、Web of Science、CNKI、万方、维普数据库，英文检索词为“animal model， osteoarthritis knee，rabbit”；中文检索词为 “膝骨关节炎，动物模型，兔”，语言分别设为中文和英文。查找1983年8月至2017年12月期间有关兔骨关节炎动物模型的文章，包括综述、基础研究以及临床研究。通过阅读文题和摘要进行初步筛选，排除与文章主题不相关的文献，根据纳入标准和排除标准，最终纳入52 篇进行综述。
结果与结论：①骨关节炎动物模型主要分为自发型、诱导型、基因敲除型；②诱导型为现临床试验最常用的方法，诱导模型包括：手术及非手术方法，手术方法以前交叉韧带切断使用较多，非手术方法以石膏外固定使用较多；③诱导模型可快速、稳定的造成与人类膝关节退变相似的病理生理改变，模型主要用于药物，骨关节炎发病机制及信号通路等方面的研究；④各种造模方法均能成功建立的骨关节炎模型，但各有优缺点，需根据实验设计及实验条件合理选择。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0002-6664-8008(彭旭)

关键词: 骨关节炎, 动物模型, 自发型, 诱导, 基因敲除, 木瓜蛋白酶, 前交叉韧带切断, 研究进展, 综述, 组织构建

Abstract:

BACKGROUND: Animal models have been widely applied in the studies on osteoarthritis. Rabbits are the most commonly used animal models. In many modeling methods, inductive model is preferred because of good stability, high efficiency, and repeatability.
OBJECTIVE: To analyze the research status and application of rabbit models of knee osteoarthritis at home and abroad in recent years.
METHODS: A computer-based search of PubMed, Web of Science, CNKI, WanFang, and VIP databases was performed. The keywords were “animal model, osteoarthritis knee, rabbit” in English and Chinese, respectively. The articles concerning rabbit models of knee osteoarthritis published between August 1983 and December 2017 were retrieved, including reviews, basic researches, and clinical studies. The articles were screened preliminarily by reading the topic and abstract of the screening, and irrelative articles were excluded. Finally 52 articles were enrolled for review according to the inclusion and exclusion criteria.
RESULTS AND CONCLUSION: Animal models of osteoarthritis are mainly divided into spontaneous, inducible, gene knockout types. Inductive models are the most commonly used models in clinical trials, including surgical and non-surgical methods. The anterior cruciate ligament transaction is the commonly used surgical methed, and immobilization model is the most common non-surgical method. The induced model can quickly and steadily cause the pathophysiological changes similar to the degeneration of human knee joint, and the models are mainly used for the studies on the drugs, pathogenesis, and signaling pathways of osteoarthritis. A variety of modeling methods can successfully establish osteoarthritis model, but each has their own advantages and disadvantages, so we should make a reasonable choice in accordance with the experimental design and experimental conditions.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoarthritis, Knee Joint, Models, Animal, Tissue Engineering

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