中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (10): 1483-1487.doi: 10.3969/j.issn.2095-4344.0705

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

锌离子含量对微弧氧化涂层促成骨活性及抗菌性能的影响

张震祥
  

  1. 江苏省泰州市人民医院骨科,江苏省泰州市  225300
  • 收稿日期:2017-11-21 出版日期:2018-04-08 发布日期:2018-04-08
  • 作者简介:张震祥,江苏省泰州市人民医院骨科,江苏省泰州市 225300
  • 基金资助:
    国家自然科学基金(81401770):关节假体微弧氧化掺锌微孔纳米涂层的抗菌性能及其机制研究

Effects of zinc ion contents on pro-osteogenic and antibacterial activities of micro-arc oxidation coatings

Zhang Zhen-xiang
  

  1. Department of Orthopedics, Taizhou People’s Hospital, Taizhou 225300, Jiangsu Province, China
  • Received:2017-11-21 Online:2018-04-08 Published:2018-04-08
  • About author:Zhang Zhen-xiang, Department of Orthopedics, Taizhou People’s Hospital, Taizhou 225300, Jiangsu Province, China
  • Supported by:
    the National Natural Science Foundation of China, No. 81401770

摘要:

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文题释义:
微弧氧化:又称等离子体电解氧化、微等离子体氧化等,是通过电解液与相应电参数的组合,在铝、镁、钛等金属及其合金表面依靠弧光放电产生的瞬时高温高压作用,原位生长出以基体金属氧化物为主的陶瓷膜层。
锌离子的作用:作为人体必需的微量元素,在发挥抗菌性能中起重要作用。研究已证明,用化学改性的方法在钛表面注入锌离子,可显着降低金黄色葡萄球菌的生存及繁殖能力。此外,锌离子还具备促成骨活性,能加速骨形成。可见,锌离子不但可促进骨骼形成,而且还能抑制细菌黏附,这可满足临床需要。
 
摘要
背景:有研究发现锌离子具有促成骨活性,也有文献表明高含量的锌离子无促成骨活性,反而抑制成骨细胞的分化;还有研究报道,当锌离子被注入到钛表面后并没有有效的抑菌作用。由于存在许多争论,对锌离子的影响尚需进一步研究。
目的:观察锌离子含量对钛表面微弧氧化涂层促成骨活性及抗菌性能的影响。
方法:采用微弧氧化法在钛片表面制作涂层,使涂层中锌离子含量(原子百分数)分别为0.199%、0.574%及1.995%,分别设为低、中、高剂量组,以未经处理的钛片为对照。将MG63细胞分别接种于4组材料表面,检测细胞增殖、形态变化及碱性磷酸酶活性。将金黄色葡萄球菌分别接种于4组材料表面,接种48 h,检测抑菌率;接种24 h时,观察细菌黏附情况。
结果与结论:①培养7 d内,随着时间的推移,各组材料表面的MG63细胞数目逐渐增加,其中低剂量组细胞增殖活性最高,其次为中剂量组及对照组,高剂量组最低;②培养48 h后,对照组、低剂量组、中剂量组细胞展示正常形态及扩展的伪足,有些伪足渗透到细胞表面;高剂量组未发现正常或完整的细胞;    ③培养13 d内,低剂量细胞碱性磷酸酶活性最高,其次为中剂量组及对照组,高剂量组最低,组间两两比较差异有显著性意义(P < 0.05);④低剂量组、中剂量组、高剂量组的抗菌率分别为62.54%、69.84%、79.19%;⑤结果表明,随着锌离子含量的增加,微弧氧化涂层的促成骨活性降低,抗菌性能提高。

关键词: 钛片, 锌离子, 微弧氧化, 成骨活性, 抗菌性能, 生物材料

Abstract:

BACKGROUND: Some studies have found that zinc ions have pro-osteogenic activity, while zinc ions at high levels are also reported to inhibit the differentiation of osteoblasts instead of the pro-osteogenic activity. In some studies, zinc ions that are injected into the surface of titanium have ineffective antibacterial effects. Therefore, further investigations on zinc ion’s effects as a controversial topic are required.
OBJECTIVE: To observe the effects of zinc ion content on the pro-osteogenic and antibacterial activities of micro-arc oxidation coatings.
METHODS: The coating was made on the surface of titanium by micro-arc oxidation method. The zinc ion content (atomic percentage) in the coating was set to 0.199%, 0.574%, and 1.995%, respectively, as low, medium and high dose groups. Untreated titanium plates were used as controls. MG63 cells were seeded on the surface of four groups of materials and tested for cell proliferation, morphological changes, and alkaline phosphatase activity. Staphylococcus aureus was inoculated on the surface of four groups of materials and the antibacterial rate was detected at 48 hours after inoculation. Bacterial adhesion was observed at 24 hours after inoculation.
RESULTS AND CONCLUSION: Within 7 days of culture, the number of MG63 cells on the material surface gradually increased with time. The proliferative ability of cells was highest in the low-dose group, followed by the middle-dose and control group, and it was lowest in the high-dose group. After 48 hours of culture, the cells in the control, low-dose and middle-dose groups showed normal morphology and expanded pseudopodia, and some pseudopodia penetrated into the cell surface, while normal or intact cells were undetected in the high-dose group. Within 13 days of culture, the activity of alkaline phosphatase was the highest in the low-dose group, followed by the middle-dose and control groups, and the lowest in the high-dose group. There were significant differences in the activity of alkaline phoshatase between groups (P < 0.05). The antibacterial rate of the materials was 62.54% in the low-dose group, 69.84% in the middle-dose group, and 79.19% in the high-dose group, respectively. Findings from this study reveal that with the increase of zinc ion contents, the pro-osteogenic activity of micro-arc oxidation coatings decreased, while the antibacterial property improved.

Key words: Titanium, Zinc, Tissue Engineering

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