中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (12): 1732-1737.doi: 10.3969/j.issn.2095-4344.2016.12.009

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

左旋聚乳酸羟基磷灰石生物材料与牙周膜细胞的生物相容性

赵 宁,张现军,李 伟,董新新   

  1. 许昌市中心医院口腔科,河南省许昌市  461000
  • 收稿日期:2015-12-30 出版日期:2016-03-18 发布日期:2016-03-18
  • 作者简介:赵宁,女,1976年生,河南省临颍县人,副主任医师,主要从事口腔颌面外科研究。

Biocompatibility of poly-L-lactic acid/hydroxyapatite biomaterial with periodontal ligament cells

Zhao Ning, Zhang Xian-jun, Li Wei, Dong Xin-xin   

  1. Department of Stomatology, Xuchang Central Hospital, Xuchang 461000, Henan Province, China
  • Received:2015-12-30 Online:2016-03-18 Published:2016-03-18
  • About author:Zhao Ning, Associate chief physician, Department of Stomatology, Xuchang Central Hospital, Xuchang 461000, Henan Province, China

摘要:

文章快速阅读:

文题释义:

羟基磷灰石:又称羟磷灰石,碱式磷酸钙,是钙磷灰石的自然矿物化,是脊椎动物骨骼和牙齿的主要无机组成成分,人的牙釉质中羟基磷灰石的含量约96%,骨中约占69%。羟基磷灰石具有优良的生物相容性和生物活性,可作为一种骨骼或牙齿的诱导因子,在口腔保健领域中对牙齿具有较好的再矿化、脱敏美白作用。但羟基磷灰石材料质地较脆,机械性能较差,在塑型方面存在一定的限制且降解速度较慢。

聚乳酸:聚乳酸由乳酸单体聚合而成,有3种结构形式,左旋聚乳酸、右旋聚乳酸及两种结构的混合物。左旋聚乳酸是一种半结晶体,有一定的硬度,降解速度要快于右旋聚乳酸。但聚乳酸材料本身具有一定的缺陷,其机械强度较低且降解产物呈酸性,易引起体内炎症反应。

 

背景:将羟基磷灰石与左旋聚乳酸复合,可以很好地改善羟基磷灰石质地脆、机械性能差等不足。
目的:进一步观察左旋聚乳酸羟基磷灰石生物材料与牙周膜细胞的相容性。
方法:将细胞浓度为5×109 L-1的第4代人牙周膜细胞与左旋聚乳酸羟基磷灰石生物材料复合培养,设为实验组,设置单纯牙周膜细胞培养作为对照组。培养7 d内检测两组细胞增殖情况;培养24,48,72 h,检测细胞碱性磷酸活性;培养72 h,检测细胞Ⅰ型胶原表达强度。
结果与结论:①细胞增殖检测结果:两组培养1-7 d的吸光度值比较差异无显著性意义。②细胞碱性磷酸活性检测结果:两组组内培养48,72 h的碱性磷酸酶活性均高于培养24 h(P < 0.05),但两组间不同时间点碱性磷酸酶活性比较差异均无显著性意义。③细胞Ⅰ型胶原表达检测结果:两组Ⅰ型胶原表达强度比较差异无显著性意义。④结果表明,左旋聚乳酸羟基磷灰石生物材料具有良好的细胞相容性。 

ORCID: 0000-0003-1432-3081(赵宁)

关键词: 生物材料, 材料相容性, 左旋聚乳酸, 羟基磷灰石, 支架材料, 牙周组织, 种子细胞, 人牙周膜细胞, 生物相容性

Abstract:

BACKGROUND: Hydroxyapatite/poly-L-lactic acid composite can well improve the brittleness and mechanical properties of the hydroxyapatite.
OBJECTIVE: To study the biocompatibility of hydroxyapatite/poly-L-lactic acid composite biomaterials with periodontal ligament cells.
METHODS: Passage 4 human periodontal ligament cells at a density of 5×109/L were cultured with hydroxyapatite/poly-L-lactic acid composite as experimental group, and human periodontal ligament cells cultured alone as control group. Cell proliferation was detected within 7 days of culture; alkaline phosphatase activity was detected at 24, 48 and 72 hours of culture; and type I collagen expression was detected at 72 hours of culture.
RESULTS AND CONCLUSION: There was no significant difference in the absorbance value of cells between the two groups at 1-7 days of culture. The alkaline phosphatase activity of cells at 48 and 72 hours was significantly higher than that at 24 hours (P < 0.05) in the two groups, but there was no difference between the two groups. The expression of type I collagen had no difference between the two groups. These findings indicate that the hydroxyapatite/poly-L-lactic acid composite biomaterial has good cell compatibility.