中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (12): 1738-1744.doi: 10.3969/j.issn.2095-4344.2016.12.010

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

丝素蛋白支架修复颊黏膜缺损

王忠朝1，2，范丽苑1，3，蔡 炜1，3，蒋俊强1，2   

  1. 1西南医科大学口颌面修复重建和再生实验室，四川省泸州市  646000；西南医科大学附属口腔医院，2牙周粘膜科，3修复科，四川省泸州市  646000
  • 出版日期:2016-03-18 发布日期:2016-03-18
  • 通讯作者: 范丽苑，讲师，西南医科大学附属口腔医院修复科，四川省泸州市 646000
  • 作者简介:王忠朝，男，1980年生，四川省峨眉山市人，汉族，2008年四川大学华西口腔医学院毕业，硕士，主治医师，主要从事牙周病的病因及防治研究。
  • 基金资助:
    四川医科大学口腔医学院省级大学生创新训练计划项目(201510632020)：下颌第一前磨牙单根管显微CT研究；四川医科大学口腔医学院校级大学生创新训练计划项目(2015131)：下颌第一前磨牙单根管显微CT研究；四川医科大学附属口腔医院院级课题：舌侧与唇侧固定矫治器戴入前后牙周可疑病原菌和牙周临床指数变化的比较研究；四川医科大学2015年国家级大学生创新创业训练计划项目(201510632020)：下颌第一前磨牙单根管显微CT研究

Silk fibroin scaffold for repair of buccal mucosa defects

Wang Zhong-chao1, 2, Fan Li-yuan1, 3, Cai Wei1, 3, Jiang Jun-qiang1, 2   

  1. 1Orofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, Sichuan Province, China; 2Department of Periodontics & Oral Medicine, 3Department of Prosthodontics, Hospital of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Online:2016-03-18 Published:2016-03-18
  • Contact: Fan Li-yuan, Lecturer, Orofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Department of Prosthodontics, Hospital of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • About author:Wang Zhong-chao, Master, Attending physician, Orofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Department of Periodontics & Oral Medicine, Hospital of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:

    the Provincial-Level Innovation Training Plan for Undergraduates in the Stomatological School of Southwest Medical University, No. 201510632020; the University-Level Innovation Training Plan for Undergraduates in the Stomatological School of Southwest Medical University, No. 2015131; the Hospital-Level Project of the Hospital of Stomatology, Southwest Medical University; the National-Level Innovation Training Plan for Undergraduates in Southwest Medical University in 2015, No. 201510632020

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被引次数

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摘要：

文章快速阅读：

文题释义：

丝素蛋白：是从蚕丝中提取的天然高分子纤维蛋白，含量占蚕丝的70%-80%，含有18种氨基酸，其中甘氨酸(Gly)、丙氨酸(Ala)和丝氨酸(Ser)约占总组成的80%以上。丝素本身具有良好的机械性能和理化性质，如良好的柔韧性和抗拉伸强度、透气透湿性、缓释性等，而且经过不同处理可以得到不同的形态，如纤维、溶液、粉、膜及凝胶等。

丝素蛋白支架修复损伤创面：丝素蛋白材料支架抗原性相对较低，并不会产生排异反应，同时该材料具有天然的三维立体结构，能够有效诱导细胞按照一定的规律生长；丝素蛋白材料支架具有良好的通气性，能够为大鼠创面愈合创造良好的生物条件，从而更加有利于创面基底部位组织液渗透到创面；丝素蛋白材料支架在运用时具有一定的可吸收性，能够避免了置入支架后二次手术。

 

背景：丝素蛋白属于天然的大分子材料，含有一种与细胞黏附关系密切的特殊三肽结构，即精-甘-天冬氨酸肽结构，能促进细胞在纤维材料表面迁移、黏附和增殖，影响细胞的形态和功能。
目的：比较不同丝素蛋白支架修复大鼠颊黏膜缺损的效果。
方法：取90只SD大鼠，制作单侧颊黏膜缺损模型，随机分为3组，每组30只，实验组于颊黏膜缺损处置入多孔丝素蛋白支架，对照组于颊黏膜缺损处置入复层物理交联丝素蛋白膜，空白对照组于颊黏膜缺损处覆盖凡士林纱布并缝合。术后15 d检测创面直径；术后30 d取缺损部位，进行苏木精-伊红染色。

结果与结论：①颊黏膜创面直径检测结果：术后15 d，实验组颊黏膜创面直径显著小于对照组、空白对照组(P < 0.05)，对照组小于空白对照组(P < 0.05)。②苏木精-伊红染色结果：术后30 d，实验组上皮细胞钉突相对较多，丝素蛋白纤维部分吸收降解，成纤维细胞数明显多于对照组、空白对照组(P < 0.05)，炎性细胞数少于对照组、空白对照组(P < 0.05)。③结果证明，多孔丝素蛋白支架在颊黏膜缺损修复中能够加速上皮化，更加有利于创面愈合。 

ORCID: 0000-0002-9460-0255(王忠朝)

关键词: 生物材料, 口腔生物材料, 丝素蛋白材料支架, 复层物理交联丝素蛋白膜, 颊黏膜缺损, 修复, 应用效果, 物理性状, 创面愈合

Abstract:

BACKGROUND: Fibroin is a natural macromolecular material with Arg-Gly-Asp peptide structure that is a special tripeptide structure closely related to cell adhesion, and it can promote cell migration, adhesion, and proliferation and influence cell morphology and function.
OBJECTIVE: To compare the effects of different silk fibroin scaffolds to repair buccal mucosa defects in rats.
METHODS: Ninety Sprague-Dawley rats were selected to make unilateral buccal mucosa defect models, and randomly divided into three groups, 30 rats in each group: porous silk fibroin scaffold was implanted into the buccal mucosa defect in experimental group, multi-layered crosslinked silk fibroin film was implanted into the buccal mucosa defect in control group, and vaseline gauze was used to cover the buccal mucosa defect followed by suturing in blank control group. After 15 days, wound diameter was detected; after 30 days, bone defect tissues were taken for hematoxylin-eosin staining.
RESULTS AND CONCLUSION: At postoperative 15 days, the wound diameter was significantly smaller in the experimental group than the control and blank control groups (P < 0.05), as well as smaller in the control group than the blank control group (P < 0.05). Hematoxylin-eosin staining showed that at 30 days after operation, there were more epithelial spikes and fibroblasts, but less inflammatory cells in the experimental group than the other two groups (P < 0.05), and fibroin fibers were partially absorbed and degraded in the experimental group. These findings indicate that porous silk fibroin scaffold for buccal mucosa defect repair can accelerate epithelialization and wound healing.