中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (14): 2532-2537.doi: 10.3969/j.issn.2095-4344.2013.14.009

• 肿瘤干细胞 cancer stem cells • 上一篇    下一篇

信号通路对胃癌干细胞及胃癌发生的影响

陈  浩1,许  浪2   

  1. 1荆州市中心医院胃肠外科,湖北省荆州市  434100
    2武汉科技大学医学院病理教研室,湖北省武汉市  430064
  • 收稿日期:2012-07-24 修回日期:2012-08-29 出版日期:2013-04-02 发布日期:2013-04-02
  • 通讯作者: 许浪,博士,副教授,武汉科技大学医学院病理教研室,湖北省武汉市 430064 643025@qq.com
  • 作者简介:陈浩★,男,1980年生,湖北省仙桃市人,汉族,2008年华中科技大学毕业,硕士,主治医师,主要从事胃肠道肿瘤、腹腔镜方面的研究。 chenhaojingzhou@163.com

Signal pathway effects on gastric cancer stem cells and gastric cancer occurrence

Chen Hao1, Xu Lang2   

  1. 1 Department of Gastrointestinal Surgery, Jingzhou Central Hospital, Jingzhou  434100, Hubei Province, China
    2 Department of Pathology, Medical College, Wuhan University of Science and Technology, Wuhan  430064, Hubei Province, China
  • Received:2012-07-24 Revised:2012-08-29 Online:2013-04-02 Published:2013-04-02
  • Contact: Xu Lang, Doctor, Associate professor, Department of Pathology, Medical College, Wuhan University of Science and Technology, Wuhan 430064, Hubei Province, China 643025@qq.com
  • About author:Chen Hao★, Master, Attending physician, Department of Gastrointestinal Surgery, Jingzhou Central Hospital, Jingzhou 434100, Hubei Province, China chenhaojingzhou@163.com

摘要:

背景:Hedgehog与Wnt/β-catenin信号通路对胃癌干细胞的影响及在胃癌发生发展中的作用机制少见报道。
目的:探讨Hedgehog与Wnt/β-catenin信号通路在胃癌发生发展中的作用机制。
方法:采用肿瘤球悬浮分选法从胃癌组织标本中分选胃癌干细胞。采用免疫组化SP法检测Hedgehog 及Wnt/β-catenin信号通路主要分子SHH、GLI1、Wnt2 及β-catenin在胃癌干细胞中的表达。Spearman相关分析各细胞因子间的相关性分析。
结果与结论:SHH、GLI1、Wnt2及β-catenin 在胃癌干细胞中的阳性表达率分别为74.7%,78.3%,85.5%和83.3%,均显著高于癌旁组织的阳性表达率(P < 0.05)。各细胞因子间在胃癌干细胞中的表达均呈正相关(P < 0.05)。说明在胃癌干细胞中Hedgehog及Wnt/β-catenin信号通路均被激活,二者互相作用可能参与了胃癌的发生发展,为胃癌的干细胞治疗提供了新的研究方向。

关键词: 干细胞, 肿瘤干细胞, 胃癌, 胃癌干细胞, Hedgehog信号通路, Wnt/β-catenin信号通路, SHH, GLI1, Wnt2, β-catenin, 克隆球细胞, 免疫组化, 阳性细胞, 干细胞图片文章

Abstract:

BACKGROUND: The effect of Hedgehog and Wnt/β-Catenin signal pathway on gastric cancer stem cells and the occurrence and development of gastric cancer is rarely reported.
OBJECTIVE: To investigate the mechanism of Hedgehog and Wnt/β-Catenin signal pathway on occurrence of gastric cancer stem cells.
METHODS: Gastric cancer stem cells were selected from cell suspension in the culture system of  serum-free medium by magnetic activated cell sorting system. Immunohistochemical SP test was applied to detect the expressions of SHH, GLI1, Wnt2 and β-Catenin protein in gastric cancer stem cells. The relationship among these factors was analyzed by Spearman.  
RESULTS AND CONCLUSION: The expression positive rates of SHH, GLI1, Wnt2 and β-Catenin were 74.7%, 78.3%, 85.5% and 83.3% respectively. It was remarkably higher than that of tissues adjacent to cancer (P < 0.05). The expression of each factors had negative relationships (P < 0.05). Hedgehog and Wnt/β-Catenin signal pathway were stimulated abnormally in gastric cancer stem cells. These two pathways may have a co-relationship in gastric cancer occurrence. It provides a new research direction for the treatment of gastric cancer.

Key words: stem cells, tumor stem cells, gastric cancer, gastric cancer stem cells, Hedgehog signal pathway, Wnt/β-catenin signal pathway, SHH, GLI1, Wnt2, β-catenin, clone cells, immunohistochemistry, positive cells, stem cell photographs-containing paper

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