中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (20): 3685-3689.doi: 10.3969/j.issn.1673-8225.2012.20.017

• 肌肉肌腱韧带组织构建 tissue construction of the muscle, tendon and ligament • 上一篇    下一篇

兔肩袖损伤模型的建立及初步组织学研究**☆

李奉龙1，姜春岩1，鲁  谊1，黎广平3，卢耀甲2   

  1. 1北京积水潭医院运动损伤科，北京市 100035；2江苏省苏北人民医院骨科，江苏省扬州市 225001；    3北京市创伤骨科研究所，北京市 100035
  • 收稿日期:2011-12-07 修回日期:2012-03-03 出版日期:2012-05-13 发布日期:2012-05-13
  • 通讯作者: 卢耀甲，博士，江苏省苏北人民医院骨科，江苏省扬州市 225001 yao10jia13lu@163.com
  • 作者简介:李奉龙☆，男，1982年生，辽宁省大连市人，汉族，2009年北京大学医学部毕业，博士，主要从事肩关节外科专业的相关基础与临床研究。long_bmu@163.com
  • 基金资助:

    首都医学发展科研基金项目(2009-2062)；北京市卫生系统高层次卫生技术人才培养计划项目(2009-3-16)。

Establishment of a rotator cuff tear model in rabbits and its preliminary histological study

Li Feng-long1, Jiang Chun-yan1, Lu Yi1, Li Guang-ping3, Lu Yao-jia2   

  1. 1Department of Sports Medicine, Beijing Jishuitan Hospital, Beijing  100035, China; 2Department of Orthopedics, Northern Jiangsu People’s Hospital, Yangzhou  225001, Jiangsu Province, China; 3Beijing Research Institute of Traumatology and Orthopedics, Beijing  100035, China
  • Received:2011-12-07 Revised:2012-03-03 Online:2012-05-13 Published:2012-05-13
  • Contact: Lu Yao-jia, Doctor, Department of Orthopedics, Northern Jiangsu People’s Hospital, Yangzhou 225001, Jiangsu Province, China yao10jia13lu@163.com
  • About author:Li Feng-long☆, Doctor, Department of Sports Medicine, Beijing Jishuitan Hospital, Beijing 100035, China long_bmu@163.com
  • Supported by:

    the Capital Medicine Research and Development Foundation, No. 2009-2062*; The Training Project of High-Level Health Personnel of Beijing Health System, No. 2009-3-16* 

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摘要：

背景：兔的冈上肌形态及结构与人类相仿，在组织学上，兔肩袖肌腱包括与人类相似的由肌腱到骨的移行区域，可用于腱-骨愈合的研究。
目的：建立兔的肩袖损伤模型，探讨此模型的可行性并进行肩袖愈合的初步组织学研究。 
方法：18只骨骼成熟雄性新西兰大耳白兔，建立肩袖损伤模型后随机数字表法均分为2组：修复组造模后1周行腱-骨缝合；对照组不予修复。分别于造模后2，4，8周对冈上肌标本进行切片及苏木精-伊红染色，观察冈上肌的脂肪浸润情况和腱-骨愈合情况。 
结果与结论：兔冈上肌的解剖结构和组织结构均与人类相似，肩袖损伤模型建立和手术修复的操作过程可重复性高。两组均未观察到明显的冈上肌脂肪浸润和萎缩变性。修复组在8周时形成了新的冈上肌止点，对照组则无新止点形成。 说明急性冈上肌损伤修复后8周可形成新止点，兔是建立肩袖损伤模型的合适动物并适用于肩袖疾病的研究。

关键词: 肩关节, 肩袖损伤, 动物模型, 兔, 组织学, 组织构建

Abstract:

BACKGROUND: The modal and structural characteristics of supraspinatus in rabbits are similar to human. In the rotator cuff of rabbits there are tendon-bone transitional zones similar to human, and it is appropriate to investigate the tendon-bone healing aspects with a rabbit model. 
OBJECTIVE: To establish an animal model of rotator cuff tear in rabbits and to investigate the feasibility of this model followed by the initial histological study. 
METHODS: Supraspinatus tenotomy was performed in the right shoulder of 18 skeletally matured male New Zealand white rabbits to establish the animal model of rotator cuff tear. The rabbits were randomly divided into two groups: repair group, repaired at 1 week after tenotomy; control group, without repair. The rabbits were sacrificed at 2, 4 and 8 weeks postoperatively in each group with three rabbits sacrificed at each time point. Fatty infiltration of supraspinatus and healing of tendon-bone interface were observed by hematoxylin-eosin staining. 
RESULTS AND CONCLUSION: The gross anatomy and histological characteristics of the supraspinatus in rabbits were similar to human. The animal model provided a stable surgical approach which ensured a highly replicable procedure in the establishment of rotator cuff tear/repair models. There was no obvious fatty infiltration or muscle atrophy in the supraspinatus in both groups. At 8 weeks after repair, we observed the formation of a new enthesis of supraspinatus in the repair group. There was no new enthesis of supraspinatus in the control group. The rabbit is suitable to establish an animal model of rotator cuff tears. The animal model designed in this study could be used in the research of rotator cuff tears. For the acute supraspinatus tear, formation of a new enthesis can be observed at 8 weeks after repair.

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