中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (20): 3690-3693.doi: 10.3969/j.issn.1673-8225.2012.20.018

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高脂饲料喂养加静脉注射小牛血清白蛋白建立兔动脉粥样硬化模型**★

李  琳，窦健霖，楚天舒，李婉秋，张  戈，孙  林   

  1. 昆明医学院第二附属医院心内科，云南省心血管病研究所冠心病基础研究室，云南省昆明市 650101
  • 收稿日期:2012-03-01 修回日期:2012-04-05 出版日期:2012-05-13 发布日期:2012-05-13
  • 通讯作者: 孙林，博士，副教授，硕士生导师，昆明医学院第二附属医院心内科，云南省心血管病研究所冠心病基础研究室，云南省昆明市 650101 sunlinkm@sina.com
  • 作者简介:李琳★，女，1985年生，山东省聊城市人，汉族，硕士，2011年昆明医学院毕业。751065905@qq.com
  • 基金资助:

    课题受云南省应用基础研究面上项目基金资助(2008CD122)及云南省教育厅科学研究基金重点项目资助(2010Z034)。

Establishment of an experimental atherosclerosis model by feeding high-fat diet plus injecting bovine serum albumin in rabbits

Li Lin, Dou Jian-lin, Chu Tian-shu, Li Wan-qiu, Zhang Ge, Sun Lin   

  1. Department of Cardiology, the Second Affiliated Hospital of Kunming Medical University, Kunming  650101, Yunnan Province, China
  • Received:2012-03-01 Revised:2012-04-05 Online:2012-05-13 Published:2012-05-13
  • Contact: Sun Lin, Doctor, Associate professor, Master’s supervisor, Department of Cardiology, the Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China sunlinkm@sina.com
  • About author:Li Lin★, Master, Department of Cardiology, the Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China 751065905@qq.com
  • Supported by:

     the General Foundation of Yunnan Provincial Applied Basic Research, No. 2008CD122*; the Science and Technology Research Foundation of Yunnan Provincial Education Bureau, No. 2010Z034*

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摘要：

背景：目前单纯饲喂高脂饲料建立动脉粥样硬化狭窄模型较常见。
目的：采用高脂饲料喂养加静脉注射小牛血清白蛋白建立兔动脉粥样硬化模型。
方法：分别单纯高脂饮食、高脂饲料+脂肪乳灌胃以及高脂饲料+小牛血清白蛋白3种不同的方法喂养兔构建动脉硬化模型，设立正常对照组，予普通饲料喂养。
结果与结论：各组兔血清胆固醇、三酰甘油、低密度脂蛋白和高密度脂蛋白水平较正常对照组显著升高(P < 0.01)。单纯高脂组可出现高脂血症，未发现典型的动脉粥样硬化病变；高脂+脂肪乳灌胃组可形成纤维斑块；高脂+小牛血清白蛋白组可形成较成熟的动脉粥样硬化斑块。结果证实，高脂饲料加静脉注射小牛血清白蛋白可形成较成熟的动脉粥样硬化斑块，可成功建立动脉粥样硬化兔模型。
关键词：动脉粥样硬化；小牛血清白蛋白；高脂饲料；兔；动物模型；组织工程
doi:10.3969/j.issn.1673-8225.2012.20.018

关键词: 动脉粥样硬化, 小牛血清白蛋白, 高脂饲料, 兔, 动物模型, 组织工程

Abstract:

BACKGROUND: It is commonly seen to establish a model of atherosclerotic stenosis by simply feeding high-fat diet.
OBJECTIVE: To establish an atherosclerosis model by feeding high-fat diet plus injecting bovine serum albumin in rabbits.
METHODS: Three different methods were used to establish rabbit models of atherosclerosis, including simple high-fat diet, high-fat diet+lipid milk, and high-fat diet+bovine serum albumin. Normal control group was fed with normal food.
RESULTS AND CONCLUSION: The levels of serum total cholesterol, triglyceride, low density lipoprotein and high density lipoprotein were significantly increased in the former three groups compared with normal control group (P < 0.01). The rabbits treated with simple high-fat diet possibly presented with hyperlipidemia rather than atherosclerotic lesions. The rabbits treated with high fatty feedstuff only showed hyperlipidemia without atherosclerosis lesion. The rabbits treated with high-fat diet and lipid milk showed fibrous plaque. The mature atherosclerotic plaques were presented in the rabbits treated with high-fat diet+bovine serum albumin. The mature atherosclerotic plaque may be created by high-fat diet plus bovine serum albumin that an ideal model of atherosclerosis can be established in rabbits.

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