中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (2): 257-260.doi: 10.3969/j.issn.1673-8225.2012.02.015

• 骨组织构建 • 上一篇    下一篇

脊髓3/4横断伤动物模型的建立及其电生理评价

李景德1，卢培刚2
  

  1. 1辽宁医学院解放军济南军区总医院研究生培养基地，山东省济南市  250031； 2解放军济南军区总医院神经外科，山东省济南市  250031
  • 收稿日期:2011-08-25 修回日期:2011-10-26 出版日期:2012-01-08 发布日期:2012-01-08
  • 通讯作者: 卢培刚，博士，副教授，副主任医师，硕士生导师，解放军济南军区总医院神经外科，山东省济南市 250031。 pglu912@126.com
  • 作者简介:李景德★，男，1981年生，山东省成武县人，汉族，辽宁医学院在读硕士，医师，主要从事神经保护的基础与临床研究。 lijingde1981@163.com

Establishment and electrophysiology evaluation of an animal model of 3/4 crosscut spinal cordinjury

Li Jing-de1, Lu Pei-gang2   

  1. 1General Hospital ofJinan Military AreaCommand of ChinesePLA (PostgraduateBasis of LiaoningMedical University),Jinan 250031,Shandong Province,China;
    2Departmentof Neurosurgery,General Hospital ofJinan Military AreaCommand of ChinesePLA, Jinan 250031,Shandong Province,China
  • Received:2011-08-25 Revised:2011-10-26 Online:2012-01-08 Published:2012-01-08
  • Contact: Lu Pei-gang, Doctor,Associate professor,Associate chiefphysician, Master’ssupervisor,Department ofNeurosurgery,General Hospital ofJinan Military AreaCommand of ChinesePLA, Jinan 250031,Shandong Province,China pglu912@126.com
  • About author:Li Jing-de★,Studying for master’sdegree, Physician,General Hospital ofJinan Military AreaCommand of ChinesePLA (PostgraduateBasis of LiaoningMedical University),Jinan 250031,Shandong Province,China lijingde1981@163.com

PDF

436

被引次数

8

摘要：

背景：建立稳定、标准的脊髓损伤动物模型是研究脊髓损伤修复的前提。
目的：建立一种实用、标准、可靠的急性大鼠脊髓损伤模型。
方法：将60只成年雌性Wistar大鼠随机均分成两组。脊髓损伤组：打开T8胸椎对应脊髓，剪开硬膜，用特殊设计的眼科维纳斯剪横跨脊髓背侧中线剪断脊髓的后3/4，深度1.5 mm。1 h后行感觉诱发电位和运动诱发电位检查。对照组：只打开椎板，剪开硬膜，暴露脊髓进行假手术。
结果与结论：造模成功率为100%。对照组造模后1周功能恢复接近正常；脊髓损伤组造模后第2周开始恢复，到第4周基本停止，最终运动功能评分未超过10分，两组比较差异有显著性意义。脊髓损伤组可以明显看到感觉诱发电位与运动诱发电位的波幅值急剧降低，且潜伏期明显延长，差异有显著性意义(P < 0.01)。说明3/4横断伤急性大鼠脊髓损伤模型制作法操作简便、重复性好，是研究脊髓再生修复的理想模型。

关键词: 脊髓损伤, 动物模型, 电生理, BBB评分, 再生修复

Abstract:

BACKGROUND: Establishing a stable and standard animal model of spinal cord injury is a precondition to investigate the repairof spinal cord injury.
OBJECTIVE: To establish a practical, standard and dependable model of acute spinal cord injury in rats.
METHODS: A total of 60 female Wistar rats were randomly assigned into two groups, spinal cord injury group and control group.The spinal cord corresponding to T8 thoracic vertebrae of spinal cord injury rats were open. The dura mater was sheared opening.The posterior 3/4 of the spinal cord was cut across the spinal dorsal midline by a specially designed Venus eye scissors, 1.5 mmin depth. The sensory evoked potentials and motor evoked potentials were examined in an hour. As for the control rats, thevertebral lamina and dura mater were opened. The spinal cord was exposed for sham operation.
RESULTS AND CONCLUSION: The success rate of model construction was 100%. The function recovery of control group wasclose to normal in the 1st week after model establishment. The function recovery of spinal cord injury group started in the 2nd weekafter model establishment and virtually stopped in the 4th week. The final motor-function grades were no more than 10. There wassignificant difference between the two groups. The wave amplitude of sensory evoked potentials and motor evoked potentials inspinal cord injury group decreased sharply and significantly, the latency extended significantly (P < 0.01). These findings indicatethat the establishment of rat acute spinal cord injury model of 3/4 traumatic transection is easy and reproducible. It is an idealmodel for the investigation of spinal regenerative repair.

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